trans-10,cis-12-Conjugated Linoleic Acid Affects Expression of Lipogenic Genes in Mammary Glands of Lactating Dairy Goats

The objective of this experiment was to investigate the effects of forage species and feeding systems on ruminal fermentation, microbiota (Butyrivibrio fibrisolvens, Fibrobacter succinogenes and total bacteria) and conjugated linoleic acid (CLA) concentration of milk in dairy goats. Twenty female crossbred Saanen lactating goats (~35 ± 3.0 kg bodyweight) in early to mid-lactation stage were assigned to a 45-day completely randomised-design feeding experiment, with the following four forage (roughage) treatments: cut-and-carry grass (CG), grazing of grass (GG), cut-and-carry leucaena (CL) and grazing of leucaena (GL; n = 5). All animals were given concentrate equivalent to 1.5% of their bodyweight. The grass used in the study was napier Pak Chong 1 grass (Pennisetum purpureum × Pennisetum americanum hybrid). The results showed that irrespective of the type of forage, grazing goats (GG and GL) had a higher (P B. fibrisolvens, F. succinogenes and total bacteria were significantly (P P B. fibrisolvens (involved in the synthesis of milk CLA), resulting in a higher milk yield, and enhances c9, t11 and t10, c12 CLA in goat milk.

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Trans-10, cis-12 conjugated linoleic acid reduces milk fat content and lipogenic gene expression in the mammary gland of sows without altering litter performance

British Journal Of Nutrition ◽

10.1017/s0007114519003325 ◽

2019 ◽

Vol 123 (6) ◽

pp. 610-618 ◽

Cited By ~ 2

Author(s):

E. C. Sandri ◽

K. J. Harvatine ◽

D. E. Oliveira

Keyword(s):

Gene Expression ◽

Mammary Gland ◽

Fatty Acid ◽

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Milk Fat ◽

Fat Content ◽

Lipogenic Gene ◽

Lipogenic Genes ◽

Lipogenic Gene Expression

AbstractTrans-10, cis-12 conjugated linoleic acid (CLA) decreases milk fat synthesis in lactating sows and involves, at least in part, the down-regulation of lipogenic genes. The objective was to evaluate the effect of CLA on milk composition and lipogenic gene expression. Twenty multiparous sows were randomly assigned to one of the two treatments for 18 d (from day 7 to day 25 of lactation): (1) control (no CLA added) and (2) 1 % of CLA mixed into the ration. CLA treatment decreased milk fat and protein content by 20 % (P = 0·004) and 11 % (P = 0·0001), respectively. However, piglet weight did not differ between treatments (P = 0·60). Dietary CLA increased the concentration of SFA in milk fat by 16 % (P < 0·0001) and decreased MUFA by 17·6 % (P < 0·0001). In the mammary gland, CLA reduced gene expression of acetyl-CoA carboxylase-α by 37 % (P = 0·003), fatty acid synthase by 64 % (P = 0·002), stearoyl-CoA desaturase 1 by 52 % (P = 0·003), lipoprotein lipase by 26 % (P = 0·03), acyl glycerol phosphate acyltransferase 6 by 15 % (P = 0·02) and diacylglycerol acyltransferase 1 by 27 % (P = 0·02), whereas the expression of fatty acid binding protein 3 was not altered by CLA treatment (P = 0·09). Mammary expression of casein-β and α-lactalbumin was reduced by CLA by 68 % (P = 0·0004) and 62 % (P = 0·005), respectively. Additionally, CLA had no effect on the expression of lipogenic genes evaluated in adipose tissue. In summary, CLA reduced milk fat content without negatively affecting litter performance and it affected mammary expression of genes involved in all lipogenic pathways studied.

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JNK inhibitor SP600125 attenuates trans‐10, cis‐12 (10,12) conjugated linoleic acid (CLA)‐ mediated inflammation and suppression of adipogenic and lipogenic genes in human (pre)adipocytes

The FASEB Journal ◽

10.1096/fasebj.23.1_supplement.717.17 ◽

2009 ◽

Vol 23 (S1) ◽

Author(s):

Arion Kennedy ◽

K. Martinez ◽

T. West ◽

M. McIntosh

Keyword(s):

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Lipogenic Genes

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Effects of forage species and feeding systems on rumen fermentation, microbiota and conjugated linoleic acid content in dairy goats

Animal Production Science ◽

10.1071/an18232 ◽

2019 ◽

Vol 59 (12) ◽

pp. 2147 ◽

Cited By ~ 1

Author(s):

Saranpong Thongruang ◽

Pramote Paengkoum ◽

Wisitiporn Suksombat ◽

Smerjai Bureenok

Keyword(s):

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Milk Yield ◽

Rumen Fermentation ◽

Pennisetum Purpureum ◽

Dairy Goats ◽

Forage Species ◽

Forage Intake ◽

Total Bacteria ◽

Feeding Systems

The objective of this experiment was to investigate the effects of forage species and feeding systems on ruminal fermentation, microbiota (Butyrivibrio fibrisolvens, Fibrobacter succinogenes and total bacteria) and conjugated linoleic acid (CLA) concentration of milk in dairy goats. Twenty female crossbred Saanen lactating goats (~35 ± 3.0 kg bodyweight) in early to mid-lactation stage were assigned to a 45-day completely randomised-design feeding experiment, with the following four forage (roughage) treatments: cut-and-carry grass (CG), grazing of grass (GG), cut-and-carry leucaena (CL) and grazing of leucaena (GL; n = 5). All animals were given concentrate equivalent to 1.5% of their bodyweight. The grass used in the study was napier Pak Chong 1 grass (Pennisetum purpureum × Pennisetum americanum hybrid). The results showed that irrespective of the type of forage, grazing goats (GG and GL) had a higher (P < 0.05) forage intake and, thus, total dry-matter and crude-protein intakes than did those fed indoors (CG and CL). However, the intake of C18:2n6 and particularly of C18:3n3 was generally higher for grass-fed goats than for leucaena-fed goats. Treatments did not exert significant differences on rumen fermentation characteristics. However, the populations of B. fibrisolvens, F. succinogenes and total bacteria were significantly (P < 0.05) higher in grazing goats (GG and GL) than in their counterparts fed with the cut-and-carry system (CG and CL). Goats in the grazing system also had a higher (P < 0.05) milk yield, and milk fat, c9, t11 CLA and omega-3 fatty acid concentrations than did those in the cut-and-carry system and only grazing goats produced detectable levels of t10, c12 CLA in milk. Putting the above together, it can be concluded that allowing dairy goats to outdoor grazing stimulates a higher forage intake, including that of C18:2n6 and C18:3n3, as well as enhancing population of B. fibrisolvens (involved in the synthesis of milk CLA), resulting in a higher milk yield, and enhances c9, t11 and t10, c12 CLA in goat milk.

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Feeding Soybean Oil to Dairy Goats Increases Conjugated Linoleic Acid in Milk

Journal of Dairy Science ◽

10.3168/jds.2007-0753 ◽

2008 ◽

Vol 91 (6) ◽

pp. 2399-2407 ◽

Cited By ~ 52

Author(s):

M.A. Bouattour ◽

R. Casals ◽

E. Albanell ◽

X. Such ◽

G. Caja

Keyword(s):

Linoleic Acid ◽

Soybean Oil ◽

Conjugated Linoleic Acid ◽

Dairy Goats

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In Utero Exposure to trans-10, cis-12 Conjugated Linoleic Acid Modifies Postnatal Development of the Mammary Gland and its Hormone Responsiveness

Journal of Mammary Gland Biology and Neoplasia ◽

10.1007/s10911-021-09499-y ◽

2021 ◽

Cited By ~ 1

Author(s):

Grace E. Berryhill ◽

Julia M. Gloviczki ◽

Josephine F. Trott ◽

Jana Kraft ◽

Adam L. Lock ◽

...

Keyword(s):

Mammary Gland ◽

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Postnatal Development ◽

Maternal Nutrition ◽

Mammary Glands ◽

In Utero ◽

Dietary Fatty Acids ◽

Postnatal Exposure ◽

Ovariectomized Mice

AbstractWe previously showed that dietary trans-10, cis-12 conjugated linoleic acid (10,12 CLA) stimulates estrogen-independent mammary growth in young ovariectomized mice. Here we investigated the effects of in utero or postnatal exposure to cis-9, trans-11 (9,11 CLA) and 10,12 CLA on postnatal development of the mammary gland and its responsiveness to ovarian steroids. In the first experiment we fed dams different CLA prior to and during gestation, then cross fostered female pups onto control fed dams prior to assessing the histomorphology of their mammary glands. Pregnant dams in the second experiment were similarly exposed to CLA, after which their female pups were ovariectomized then treated with 17β-estradiol (E), progesterone (P) or E + P for 5 days. In a third experiment, mature female mice were fed different CLA for 28 days prior to ovariectomy, then treated with E, P or E + P. Our data indicate that 10,12 CLA modifies the responsiveness of the mammary glands to E or E + P when exposure occurs either in utero, or postnatally. These findings underline the sensitivity of the mammary glands to dietary fatty acids and reinforce the potential for maternal nutrition to impact postnatal development of the mammary glands and their risk for developing cancer.

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