Advanced Design of Microfluidic Chip Based on SPP-LSP Plasmonic Coupling for SERS Detection with High Sensitivity and Reliability

A low-cost b̲a̲ttery-powered s̲pectrophotometric s̲ystem (BASS) was developed for high-sensitivity point-of-care analysis in low-resource settings on a microfluidic chip without relying on external power supplies.

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Plasmonic Coupling Interference: a new approach for cancer diagnostics using SERS detection

10.1117/12.917630 ◽

2012 ◽

Author(s):

Hsin-Neng Wang ◽

Tuan Vo-Dinh

Keyword(s):

Cancer Diagnostics ◽

New Approach ◽

Plasmonic Coupling ◽

Sers Detection

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Surface-enhanced Raman scattering on a zigzag microfluidic chip: towards high-sensitivity detection of As(iii) ions

Analytical Methods ◽

10.1039/c3ay42283f ◽

2014 ◽

Vol 6 (12) ◽

pp. 4077-4082 ◽

Cited By ~ 20

Author(s):

Nan Qi ◽

Bowei Li ◽

Huiyan You ◽

Wei Zhang ◽

Longwen Fu ◽

...

Keyword(s):

Sensitivity Analysis ◽

Raman Scattering ◽

Microfluidic Chip ◽

High Sensitivity ◽

Surface Enhanced Raman Scattering ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Enhanced Raman Scattering ◽

High Sensitivity Detection ◽

Sensitivity Detection

A quantitative sensitivity analysis for trace As(iii) ions was proposed based on the SERS technique on a zigzag microfluidic chip.

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Quantitative SERS Detection of Trace Glutathione with Internal Reference Embedded Au-core/Ag-shell Nanoparticles

Nano LIFE ◽

10.1142/s1793984416420034 ◽

2016 ◽

Vol 06 (03n04) ◽

pp. 1642003 ◽

Cited By ~ 2

Author(s):

Yan Zhou ◽

Rui Ding

Keyword(s):

Reference Method ◽

High Sensitivity ◽

Internal Reference ◽

Shell Nanoparticles ◽

New Strategy ◽

Surface Enhanced Raman ◽

Sers Detection ◽

Quantitative Sers ◽

Shell Particles ◽

Improved Accuracy

Surface-enhanced Raman scattering (SERS) has been widely studied and applied for over three decades. However, reliable SERS detection of molecules with low polarizability is still suffering from poor sensitivity and reproducibility. In this paper, we have reported a new strategy for performing quantitative SERS detection of Raman insensitive Glutathione (GSH), based on GSH-induced replacement of a highly Raman sensitive four-mercaptopyridine (MP) adsorbed on the surface of four-aminothiophenol (ATP) embedded Au-core/Ag-shell particles. This replacement led to a strong decrease of the MP SERS signal, which was used to determine the concentration of GSH. The adoption of GSH-induced Raman probe replacement leads to high sensitivity, while the use of internal reference method provides an improved accuracy of the GSH quantification.

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Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

10.21203/rs.2.21078/v3 ◽

2020 ◽

Author(s):

Yan Jiang ◽

Shan Jiang ◽

Yue Wu ◽

Bin Zhou ◽

Kaiming Wang ◽

...

Keyword(s):

High Throughput ◽

Microfluidic Chip ◽

Detection System ◽

Sampling Site ◽

High Sensitivity ◽

Pcr Detection ◽

Clinical Samples ◽

Pathogenic Microorganisms ◽

Real Time Quantitative Pcr ◽

Inspection And Quarantine

Abstract Background： At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process had the risks of degradation of biological samples and generation of pathogenic microorganisms and did not meet the rapid on-site detection demand because it took a rather long time. Therefore, it is urgently needed to develop a rapid and high-throughput detection assay of pathogenic microorganisms at the customs port. The aim of this study was to develop a microfluidic chip to rapidly detect swine pathogenic microorganisms with high-throughput and higher accuracy. Moreover, this chip will decrease the risk of spreading infection during transportation.Results: A series of experiments were performed to establish a microfluidic chip. The resulting data showed that the positive nucleic acid of four swine viruses were detected by using a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Furthermore, the detection results of eight clinical samples were obtained within an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results showed that the high sensitivity and specificity of this chip system in disease detection played an important role in customs inspection and quarantine during customs clearance.Conclusion: The microfluidic PCR detection system established in this study could meet the requirement for rapid detection of samples at the customs port. This chip could avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reduce the inspection cycle. Moreover, it would enable parallel inspections on one chip, which greatly raised the efficiency of inspection.

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Epitaxial Graphene Sensors Combined with 3D Printed Microfluidic Chip for Heavy Metals Detection

Proceedings ◽

10.3390/proceedings2130982 ◽

2018 ◽

Vol 2 (13) ◽

pp. 982 ◽

Cited By ~ 2

Author(s):

Maria Francesca Santangelo ◽

Ivan Shtepliuk ◽

Donatella Puglisi ◽

Daniel Filippini ◽

Rositsa Yakimova ◽

...

Keyword(s):

Heavy Metals ◽

Microfluidic Chip ◽

Chemical Interaction ◽

High Sensitivity ◽

Epitaxial Graphene ◽

Phase Detection ◽

Lab On Chip ◽

Sensing Platform ◽

3D Printed ◽

On Chip

Two-dimensional materials may constitute key elements in the development of a sensing platform where extremely high sensitivity is required, since even minimal chemical interaction can generate appreciable changes in the electronic state of the material. In this work, we investigate the sensing performance of epitaxial graphene on Si-face 4H-SiC (EG/SiC) for liquid-phase detection of heavy metals (e.g., Pb). The integration of preparatory steps needed for sample conditioning is included in the sensing platform, exploiting fast prototyping using a 3D printer, which allows direct fabrication of a microfluidic chip incorporating all the features required to connect and execute the Lab-on-chip (LOC) functions. It is demonstrated that interaction of Pb2+ ions in water-based solutions with the EG enhances its conductivity exhibiting a Langmuir correlation between signal and Pb2+ concentration. Several concentrations of Pb2+ solutions ranging from 125 nM to 500 µM were analyzed showing good stability and reproducibility over time.

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Culturing and Bonding of Diatom on a Microfluidic Chip for Biosensing Application

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.461.809 ◽

2013 ◽

Vol 461 ◽

pp. 809-813 ◽

Cited By ~ 1

Author(s):

Jun Cai ◽

Jun Feng Pan ◽

Ming Li Chen ◽

Yu Wang ◽

De Yuan Zhang

Keyword(s):

Organic Matter ◽

Fluorescence Intensity ◽

Microfluidic Chip ◽

Ultraviolet Irradiation ◽

High Sensitivity ◽

Bonding Mechanism

In order to fabricate biosensors with high sensitivity, the diatom Coscinodiscus excentricus was cultured on a microfluidic chip made with polydimethylsiloxane (PDMS), then the organic matter of diatom cells was removed and only diatom frustules were left, after which the cleaned frustules were bonded onto the bottom of the PDMS micro chamber by ultraviolet irradiation. The overview and the detailed morphology of the frustules in the chamber were analyzed by SEM, and the ultraviolet irradiation bonding mechanism of diatom with PDMS was discussed. Finally, the diatom based microfluidic chip was tested with the fluorescein-labeled protein; and the result showed that the fluorescence intensity of the diatom is 4 times stronger compared with that of the blank regions.

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