Cationic Amphiphiles with Specificity against Gram-Positive and Gram-Negative Bacteria: Chemical Composition and Architecture Combat Bacterial Membranes

The genera Cistus present groups of about twenty species of shrubs belon- ging to the Cistaceae family are very common throughout the Mediterranean region. Cistus salviifolius, known as Melliya is a species of this genus that occurs in the forest region of Mount Ouarsenis (northwest Algeria), exactly in Thniet El Had Na onal Park in the Wilaya of Tissemsilt. This study was un- dertaken to evaluate the an bacterial effects of C. salviifolius and its chemical composition in addition the effect of shade drying me on essen al oil yield in order to op mize this parameter which is most o en not controlled for producers. The op mal yield recorded is about 0.07± 0.02%. Its characte- riza on by gas chromatography coupled with mass spectrometry shows a dominance of hydrocarbon compounds. The minimum inhibitory concentration (MIC) is established using 6 strains of Gram-positive and negative bacteria. The antibiotic susceptibility test is performed using the giliose- environment diffusion method, a bacterial suspension is prepared and subsequently well homogenized. Opacity must be adjusted to 0.5 McF (McFarland), from a young exponen al culture (18 to 24 hours). The an mi- crobial ac vity of C. salviifolius EO was tested against three Gram-posi ve and three Gram-nega ve bacteria. In fact, a strong inhibitory and bactericidal effect has been demonstrated against all Gram-positive and Gram- negative bacteria tested. It appears from this study that the EO of C. salviifo- lius has a very high antimicrobial activity, it shows a wide spectrum covering Gram positive and Gram negative bacteria. Taking into account the results of this study, new an bacterial agents could be developed and the use of C.salviifolius should be encouraged in the pharmaceutical and perfumery industries.

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Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

Natural Product Communications ◽

10.1177/1934578x1200700530 ◽

2012 ◽

Vol 7 (5) ◽

pp. 1934578X1200700 ◽

Cited By ~ 2

Author(s):

Alexis Peña ◽

Luis Rojas ◽

Rosa Aparicio ◽

Libia Alarcón ◽

José Gregorio Baptista ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

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Chemical Composition, Olfactory Evaluation and Antimicrobial Activities of Jasminum grandiflorum L. Absolute from India

Natural Product Communications ◽

10.1177/1934578x0700200411 ◽

2007 ◽

Vol 2 (4) ◽

pp. 1934578X0700200 ◽

Cited By ~ 7

Author(s):

Leopold Jirovetz ◽

Gerhard Buchbauer ◽

Thomas Schweiger ◽

Zapriana Denkova ◽

Alexander Slavchev ◽

...

Keyword(s):

Candida Albicans ◽

Chemical Composition ◽

Antimicrobial Activities ◽

Biological Data ◽

Gram Negative Bacteria ◽

Positive Bacterium ◽

Gram Positive ◽

Gram Negative ◽

Agar Dilution ◽

Minor Compounds

The chemical composition of a sample of Jasminum grandiflorum L. absolute from India was analyzed by GC and GC/MS. The major compounds identified were benzyl acetate (23.7%), benzyl benzoate (20.7%), phytol (10.9%), linalool (8.2%), isophytol (5.5%), geranyl linalool (3.0%), methyl linoleate (2.8%) and eugenol (2.5%). The odor of this absolute sample was olfactorically evaluated as intense and heavy floral-flowery, with penetrant-animalic and fruity side-notes. Some main and minor compounds were found to be responsible for the aroma impression of this sample. The antimicrobial activities of the J. grandiflorum sample and of some of its main and minor compounds were tested against Gram-positive and Gram-negative bacteria, as well as against the yeast Candida albicans, using agar dilution and agar diffusion methods. The jasmine absolute showed medium to high activity (reference compounds: eugenol and three synthetic antibiotics) against the Gram-positive bacterium Enterococcus faecalis, against the Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella sp., as well as against the yeast Candida albicans. A comparison of these biological data with those of some constituents of the jasmine absolute is also given.

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Mechanisms of resistance to membrane-disrupting antibiotics in Gram-positive and Gram-negative bacteria

MedChemComm ◽

10.1039/c5md00389j ◽

2016 ◽

Vol 7 (1) ◽

pp. 86-102 ◽

Cited By ~ 27

Author(s):

Kfir B. Steinbuch ◽

Micha Fridman

Keyword(s):

Bacterial Membrane ◽

Gram Negative Bacteria ◽

Mechanisms Of Resistance ◽

Gram Positive ◽

Gram Negative ◽

Cationic Amphiphiles

A diverse repertoire of mechanisms has evolved to confer resistance to bacterial membrane disrupting antimicrobial cationic amphiphiles.

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Distinct antibacterial activity of a vertically aligned graphene coating against Gram-positive and Gram-negative bacteria

Journal of Materials Chemistry B ◽

10.1039/d0tb00417k ◽

2020 ◽

Vol 8 (28) ◽

pp. 6069-6079

Author(s):

Wei Wei ◽

Jiurong Li ◽

Zeyang Liu ◽

Yuan Deng ◽

Da Chen ◽

...

Keyword(s):

Antibacterial Activity ◽

Gram Negative Bacteria ◽

Antibacterial Mechanism ◽

Si Substrate ◽

Gram Positive ◽

Gram Negative ◽

Bacterial Membranes ◽

Gram Positive Bacteria ◽

Graphene Coating ◽

Vertically Aligned

The distinct antibacterial mechanism of vertical graphene Si toward bacteria. Vertical graphene kills Gram-positive bacteria through physical disruption and Si substrate kills Gram-negative bacteria by extracting electrons from bacterial membranes.

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Chemical Composition and Antibacterial Activity of Selected Essential Oils and Some of Their Main compounds

Natural Product Communications ◽

10.1177/1934578x1000500905 ◽

2010 ◽

Vol 5 (9) ◽

pp. 1934578X1000500 ◽

Cited By ~ 2

Author(s):

Juergen Wanner ◽

Erich Schmidt ◽

Stefanie Bail ◽

Leopold Jirovetz ◽

Gerhard Buchbauer ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Plant Pathogens ◽

Food Poisoning ◽

Test Methods ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Spoilage Bacteria ◽

Different Strains

The chemical composition of essential oils of cabreuva ( Myrocarpus Fastigiatus Allemao, Fabaceae) from Brazil, cedarwood ( Juniperus ashei, Cupressaceae) from Texas, Juniper berries ( Juniperus communis L., Cupressaceae) and myrrh ( Commiphora myrrha (Nees) Engl., Burseraceae) were analyzed using GC/FID and GC/MS. The antimicrobial activity of these essential oils and some of their main compounds were tested against eleven different strains of Gram-positive and Gram-negative bacteria by using agar diffusion and agar serial dilution methods. Animal and plant pathogens, food poisoning and spoilage bacteria were selected. The volatile oils exhibited considerable inhibitory effects against all tested organisms, except Pseudomonas, using both test methods. Higher activity was observed against Gram-positive strains in comparison with Gram-negative bacteria. Cabreuva oil from Brazil showed similar results, but in comparison with the other oils tested, only when higher concentrations of oil were used.

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PAMAM-dendrimer Enhanced Antibacterial Effect of Vancomycin Hydrochloride Against Gram-Negative Bacteria

Journal of Pharmacy & Pharmaceutical Sciences ◽

10.18433/jpps29659 ◽

2018 ◽

Vol 22 ◽

pp. 10-21 ◽

Cited By ~ 8

Author(s):

Azadeh Serri ◽

Arash Mahboubi ◽

Afshin Zarghi ◽

Hamid Reza Moghimi

Keyword(s):

Antibacterial Activity ◽

Inhibitory Concentration ◽

In Vitro Release ◽

Pamam Dendrimers ◽

Dilution Method ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Bacterial Membranes ◽

Gram Positive Bacteria

Purpose: The antibacterial activity of some antibiotics is specific to either Gram-positive or Gram-negative bacteria. There are different mechanisms behind such insensitivities like inability of antibiotics to permeate through some bacterial membranes, as is the case for vancomycin in Gram-negative bacteria. The present investigation tries to overcome this problem by dendrimers, in order to make Gram-negative bacteria responsive to vancomycin. Methods: The effects of generations 3 (G3) and 5 (G5) polyamidoamine amine-terminated dendrimers (NH2-PAMAM), on the antibacterial activity of vancomycin, were evaluated. Vancomycin-PAMAM dendrimers complexes were prepared and their antibacterial activities were evaluated by determination of their “minimum inhibitory concentration (MIC)”, “minimum bactericidal concentration” and “fractional inhibitory concentration index” values against two Gram-positive and four Gram-negative bacteria, using broth micro-dilution method. The complexation of vancomycin and dendrimers was also assessed by in vitro release studies across dialysis tubing using a developed HPLC method. Results: Results showed that vancomycin solution was effective against Gram-positive bacteria, but, was not effective in Gram-negative ones. Vancomycin-PAMAM dendrimers exhibited significant antibacterial efficacy against Gram-negative bacteria resulting in a decline of vancomycin MIC values by about 2, 2, 4 and 64 times in E. coli, K. pneumonia, S. typhimurium and P. aeruginosa, respectively. Results also showed that enhanced effect by G5 is more than G3. Dendrimers did not affect antibacterial activity of vancomycin in Gram-positive bacteria, as no permeation problem exists here. Conclusions: The present study revealed that both G3 and G5 cationic PAMAM dendrimers are able to make Gram-negative bacteria sensitive to vancomycin, resulting in decline of MIC values up to 64 times, possibly by increasing its permeation through bacterial membrane. These results look promising for broadening the antibacterial spectrum of vancomycin and such a strategy might be used for increasing the overall life of antibiotics.

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Chemical composition, antimicrobial and antioxidant potential of the essential oil of Guarea kunthiana A. Juss

Brazilian Journal of Biology ◽

10.1590/1519-6984.04116 ◽

2017 ◽

Vol 78 (1) ◽

pp. 53-60 ◽

Cited By ~ 4

Author(s):

J. A. Pandini ◽

F. G. S. Pinto ◽

M. C. Scur ◽

C. B. Santana ◽

W. F. Costa ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Antioxidant Properties ◽

Antioxidant Potential ◽

General Point ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Broth Microdilution Method

Abstract The essential oils are extracted from plant compounds and can present activities antimicrobial and antioxidant properties. The goals of the present study were: (a) to determine the chemical composition of the essential oil of Guarea kunthiana A. Juss using the method of gas chromatography coupled to mass spectrometry (GC-MS); (b) to evaluate the antimicrobial potential of this oil using the broth microdilution method against different microorganisms: five Gram-negative bacteria, four Gram-positive bacteria and a yeast and (c) to determine the antioxidant activity of the oil using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay. The GC-MS analyses allowed identifying 13 constituents, representing 96.52% of the essencial oil composition. The main compounds identified were α-zingiberene (34.48%), β-sesquiphellandrene (22.90%), and α-curcumene (16.17%). With respect to the antimicrobial activity, the essential oil was effective against all the microorganisms tested, except for the bacteria E. coli and K. pneumoniae, which were resistant to the action of the oil. From a general point of view, Gram-positive bacteria were more susceptible to the action of the essential oil than Gram-negative bacteria. The essential oil exhibited antioxidant potential.

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Synthesis and evaluation of membrane permeabilizing properties of cationic amphiphiles derived from the disaccharide trehalose

Organic & Biomolecular Chemistry ◽

10.1039/c6ob00031b ◽

2016 ◽

Vol 14 (11) ◽

pp. 3012-3015 ◽

Cited By ~ 8

Author(s):

P. Shaul ◽

R. I. Benhamou ◽

I. M. Herzog ◽

S. Louzoun Zada ◽

Y. Ebenstein ◽

...

Keyword(s):

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Cationic Amphiphiles ◽

Bacteria And Fungi

Cationic amphiphiles derived from trehalose have been synthesized; trehalose analogues substituted withn-pentyl orn-hexyl ethers exhibited membrane disrupting activities against clinically important Gram positive and Gram negative bacteria and fungi.

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Chemical Composition and Antibacterial Activity of the Essential Oil of Ruilopezia bracteosa

Natural Product Communications ◽

10.1177/1934578x1501000432 ◽

2015 ◽

Vol 10 (4) ◽

pp. 1934578X1501000 ◽

Cited By ~ 1

Author(s):

Libia Alarcón ◽

Alexis Peña ◽

Judith Velasco ◽

José Gregorio Baptista ◽

Luis Rojas ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Gram Negative Bacteria ◽

Broth Microdilution ◽

Gram Positive ◽

Gram Negative ◽

Agar Diffusion

The essential oil from the leaves of Ruilopezia bracteosa was obtained by hydrodistillation, and analyzed by GC-MS. Eighteen components, which made up 99.6% of the oil were identified, the most abundant being β-myrcene (34.2%), α-pinene (24.3%), 7-epi-α-selinene (9.1%), β-pinene (8.5%) and 6,9-guaiadiene (4.4%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using broth microdilution and disk agar diffusion methods. MIC values found presented significant differences between both methods, which may be due to diffusion factors.

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