Prevention of Bacterial Colonization on Catheters by a One-Step Coating Process Involving an Antibiofouling Polymer in Water

2017 ◽  
Vol 9 (23) ◽  
pp. 19736-19745 ◽  
Author(s):  
Hyeongseop Keum ◽  
Jin Yong Kim ◽  
Byeongjun Yu ◽  
Seung Jung Yu ◽  
Jinjoo Kim ◽  
...  
RSC Advances ◽  
2015 ◽  
Vol 5 (66) ◽  
pp. 53802-53808 ◽  
Author(s):  
Jian Li ◽  
Long Yan ◽  
Haoyu Li ◽  
Jianping Li ◽  
Fei Zha ◽  
...  

Superhydrophobic attapulgite coated mesh was used to separate oil/water mixtures efficiently. Besides, the separation mechanism was elaborated by interpreting the different states of water droplet on the surface before and during separation.


2020 ◽  
Vol MA2020-02 (27) ◽  
pp. 1899-1899
Author(s):  
Mijoung Kim ◽  
Moonhoe Kim ◽  
Gisung Kim ◽  
Jaekwon Shin ◽  
Juyoung Oh ◽  
...  

Langmuir ◽  
2015 ◽  
Vol 31 (39) ◽  
pp. 10702-10707 ◽  
Author(s):  
Jian Li ◽  
Runni Wu ◽  
Zhijiao Jing ◽  
Long Yan ◽  
Fei Zha ◽  
...  

Pharmaceutics ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 662 ◽  
Author(s):  
Shwe Phyu Hlaing ◽  
Jihyun Kim ◽  
Juho Lee ◽  
Dongmin Kwak ◽  
Hyunwoo Kim ◽  
...  

Due to their low acid tolerance, a majority of probiotics face diculties with regard tosurviving in the gastric environment long enough to reach the intestinal surfaces where they colonizeand provide health benefits. We prepared a probiotic delivery system that can enhance their viabilityin acidic conditions by developing a one-step poly-L-lysine (PLL) coating process. We determinedwhether the coating process was successful by measuring the zeta potential and observing it withconfocal scanning microscopy. PLL-coated L. plantarum (PLL-LP), incubated in a solution of pH 2 for2 h, exhibited a higher viability (6.86 0.12 log CFU/mL of viable cells) than non-coated L. plantarum(non-coated LP), which exhibited only 2.7 1.23 log CFU/mL of viable cells. In addition, a higheramount of L. plantarum was detected in the feces of mice orally administered PLL-LP (6.2 0.4 logCFU/g of feces) than in the feces of the control groups. In addition to enhancing probiotic viability inpH 2 solution, the PLL coating showed no eect on the probiotic growth pattern and the viability ofeither freeze-dried L. plantarum or L. plantarum, stored at ?20 C and 4 C, respectively. Overall, theseresults indicated that the PLL coating is a promising potential probiotic delivery system.


2021 ◽  
Vol 22 (21) ◽  
pp. 11915
Author(s):  
Young Eun Hwang ◽  
Seonghun Im ◽  
Hyun Kim ◽  
Jung-Hoon Sohn ◽  
Byung-Kwan Cho ◽  
...  

Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent synthetic antimicrobial peptide, and the adhesiveness and antibacterial properties of the resulting peptides are evaluated. The peptide is successfully immobilized on polystyrene, titanium, and polydimethylsiloxane surfaces within 10 min in a one-step coating process with no prior surface functionalization. The antibacterial effectiveness of the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces is confirmed by complete inhibition of the growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus within 2 h. The stability of the peptide coated on the substrate surface is maintained for 84 days, as confirmed by its bactericidal activity. Additionally, the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces show no cytotoxicity toward the human keratinocyte cell line HaCaT. The antimicrobial properties of the peptide-coated surfaces are confirmed in a subcutaneous implantation animal model. The adhesive antimicrobial peptide developed in this study exhibits potential as an antimicrobial surface-coating agent for efficiently killing a broad spectrum of bacteria on contact.


Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.


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