Visible Light Photoinitiation of Cell-Adhesive Gelatin Methacryloyl Hydrogels for Stereolithography 3D Bioprinting

2018 ◽  
Vol 10 (32) ◽  
pp. 26859-26869 ◽  
Author(s):  
Zongjie Wang ◽  
Hitendra Kumar ◽  
Zhenlin Tian ◽  
Xian Jin ◽  
Jonathan F. Holzman ◽  
...  
Keyword(s):  
Visible Light ◽  
3D Bioprinting ◽  
Cell Adhesive

3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking

2018 ◽  
Vol 10 (4) ◽  
pp. 044104 ◽  
Author(s):  
D Petta ◽  
A R Armiento ◽  
D Grijpma ◽  
M Alini ◽  
D Eglin ◽  
...  
Keyword(s):  
Visible Light ◽  
3D Bioprinting

scholarly journals Visible Light-Induced 3D Bioprinting Technologies and Corresponding Bioink Materials for Tissue Engineering: A Review

Engineering ◽  
2020 ◽  
Author(s):  
Zizhuo Zheng ◽  
David Eglin ◽  
Mauro Alini ◽  
Geoff R. Richards ◽  
Ling Qin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Visible Light ◽  
3D Bioprinting

A simple and high-resolution stereolithography-based 3D bioprinting system using visible light crosslinkable bioinks

2015 ◽  
Vol 7 (4) ◽  
pp. 045009 ◽  
Author(s):  
Zongjie Wang ◽  
Raafa Abdulla ◽  
Benjamin Parker ◽  
Roya Samanipour ◽  
Sanjoy Ghosh ◽  
...  
Keyword(s):  
High Resolution ◽  
Visible Light ◽  
3D Bioprinting

scholarly journals Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Enkhtuul Gantumur ◽  
Masaki Nakahata ◽  
Masaru Kojima ◽  
Shinji Sakai
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Culture Medium ◽  
Living Cells ◽  
Cross Linking ◽  
3D Bioprinting ◽  
Cell Culture Medium ◽  
Adhesive Surface ◽  
Cell Adhesive ◽  
Human Nose

We report an extrusion-based bioprinting approach, in which stabilization of extruded bioink is achieved through horseradish peroxidase (HRP)-catalyzed cross-linking consuming hydrogen peroxide (H2O2) supplied from HRP and glucose. The bioinks containing living cells, HRP, glucose, alginate possessing phenolic hydroxyl (Ph) groups, and cellulose nanofiber were extruded to fabricate 3D hydrogel constructs. Lattice- and human nose-shaped 3D constructs were successfully printed and showed good stability in cell culture medium for over a week. Mouse 10T1/2 fibroblasts enclosed in the printed constructs remained viable after 7 days of culture. It was also able to switch a non-cell-adhesive surface of the printed construct to cell-adhesive surface for culturing cells on it through a subsequent cross-linking of gelatin possessing Ph moieties. These results demonstrate the possibility of utilizing the presented cross-linking method for 3D bioprinting.

scholarly journals Advanced Strategies for 3D Bioprinting of Tissue and Organs Analogs Using Alginate Hydrogel Bioinks

Marine Drugs ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. 708
Author(s):  
Qiqi Gao ◽  
Byoung-Soo Kim ◽  
Ge Gao
Keyword(s):  
Low Cost ◽  
Ionic Gelation ◽  
3D Bioprinting ◽  
Alginate Hydrogel ◽  
Advanced Material ◽  
Natural Polysaccharide ◽  
Cell Adhesive ◽  
Good Biocompatibility ◽  
Tissue Models

Alginate is a natural polysaccharide that typically originates from various species of algae. Due to its low cost, good biocompatibility, and rapid ionic gelation, the alginate hydrogel has become a good option of bioink source for 3D bioprinting. However, the lack of cell adhesive moieties, erratic biodegradability, and poor printability are the critical limitations of alginate hydrogel bioink. This review discusses the pivotal properties of alginate hydrogel as a bioink for 3D bioprinting technologies. Afterward, a variety of advanced material formulations and biofabrication strategies that have recently been developed to overcome the drawbacks of alginate hydrogel bioink will be focused on. In addition, the applications of these advanced solutions for 3D bioprinting of tissue/organ mimicries such as regenerative implants and in vitro tissue models using alginate-based bioink will be systematically summarized.

Studies of metaphase chromosomes in the scanning transmission x-ray microscope: Image fidelity, radiation damage and specimen preparation

1992 ◽  
Vol 50 (2) ◽  
pp. 1038-1039
Author(s):  
Shawn Williams ◽  
Xiaodong Zhang ◽  
Susan Lamm ◽  
Jack Van’t Hof
Keyword(s):  
Visible Light ◽  
Radiation Damage ◽  
Cross Section ◽  
Imaging Techniques ◽  
Dose Range ◽  
Specimen Preparation ◽  
X Rays ◽  
Metaphase Chromosomes ◽  
X Ray ◽  
Scanning Transmission

The Scanning Transmission X-ray Microscope (STXM) is well suited for investigating metaphase chromosome structure. The absorption cross-section of soft x-rays having energies between the carbon and oxygen K edges (284 - 531 eV) is 6 - 9.5 times greater for organic specimens than for water, which permits one to examine unstained, wet biological specimens with resolution superior to that attainable using visible light. The attenuation length of the x-rays is suitable for imaging micron thick specimens without sectioning. This large difference in cross-section yields good specimen contrast, so that fewer soft x-rays than electrons are required to image wet biological specimens at a given resolution. But most imaging techniques delivering better resolution than visible light produce radiation damage. Soft x-rays are known to be very effective in damaging biological specimens. The STXM is constructed to minimize specimen dose, but it is important to measure the actual damage induced as a function of dose in order to determine the dose range within which radiation damage does not compromise image quality.

Scanning luminescence x-ray microscopy: Progress toward selective staining using microspheres

1994 ◽  
Vol 52 ◽  
pp. 74-75
Author(s):  
C. Jacobsen ◽  
J. Fu ◽  
S. Mayer ◽  
Y. Wang ◽  
S. Williams
Keyword(s):  
Visible Light ◽  
Active Sites ◽  
Light Emission ◽  
Chinese Hamster ◽  
Polystyrene Spheres ◽  
Good Resistance ◽  
X Ray ◽  
Selective Staining ◽  
Visible Light Emission ◽  
Specific Labelling

In scanning luminescence x-ray microscopy (SLXM), a high resolution x-ray probe is used to excite visible light emission (see Figs. 1 and 2). The technique has been developed with a goal of localizing dye-tagged biochemically active sites and structures at 50 nm resolution in thick, hydrated biological specimens. Following our initial efforts, Moronne et al. have begun to develop probes based on biotinylated terbium; we report here our progress towards using microspheres for tagging.Our initial experiments with microspheres were based on commercially-available carboxyl latex spheres which emitted ~ 5 visible light photons per x-ray absorbed, and which showed good resistance to bleaching under x-ray irradiation. Other work (such as that by Guo et al.) has shown that such spheres can be used for a variety of specific labelling applications. Our first efforts have been aimed at labelling ƒ actin in Chinese hamster ovarian (CHO) cells. By using a detergent/fixative protocol to load spheres into cells with permeabilized membranes and preserved morphology, we have succeeded in using commercial dye-loaded, spreptavidin-coated 0.03μm polystyrene spheres linked to biotin phalloidon to label f actin (see Fig. 3).

Photocatalyst-free decarboxylative aminoalkylation of imidazo[1,2-a]pyridines with N-aryl glycines enabled by visible light

2019 ◽  
Vol 6 (21) ◽  
pp. 3693-3697 ◽  
Author(s):  
Jiu-Jian Ji ◽  
Zhi-Qiang Zhu ◽  
Li-Jin Xiao ◽  
Dong Guo ◽  
Xiao Zhu ◽  
...  
Keyword(s):  
Visible Light

A novel, green and efficient visible-light-promoted decarboxylative aminoalkylation reaction of imidazo[1,2-a]pyridines with N-aryl glycines has been described.

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