Sequential Release of Small Extracellular Vesicles from Bilayered Thiolated Alginate/Polyethylene Glycol Diacrylate Hydrogels for Scarless Wound Healing

The interaction of water within synthetic and natural hydrogel systems is of fundamental importance in biomaterial science. A systematic study is presented on the swelling behavior and states of water for a polyethylene glycol-diacrylate (PEGDA)-based model neutral hydrogel system that goes beyond previous studies reported in the literature. Hydrogels with different network structures are crosslinked and swollen in different combinations of water and phosphate-buffered saline (PBS). Network variables, polyethylene glycol (PEG) molecular weight (MW), and weight fraction are positively correlated with swelling ratio, while “non-freezable bound water” content decreases with PEG MW. The presence of ions has the greatest influence on equilibrium water and “freezable” and “non-freezable” water, with all hydrogel formulations showing a decreased swelling ratio and increased bound water as ionic strength increases. Similarly, the number of “non-freezable bound water” molecules, calculated from DSC data, is greatest—up to six molecules per PEG repeat unit—for gels swollen in PBS. Fundamentally, the balance of osmotic pressure and non-covalent bonding is a major factor within the molecular structure of the hydrogel system. The proposed model explains the dynamic interaction of water within hydrogels in an osmotic environment. This study will point toward a better understanding of the molecular nature of the water interface in hydrogels.

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More natural more better: triple natural anti-oxidant puerarin/ferulic acid/polydopamine incorporated hydrogel for wound healing

Journal of Nanobiotechnology ◽

10.1186/s12951-021-00973-7 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Qianmin Ou ◽

Shaohan Zhang ◽

Chuanqiang Fu ◽

Le Yu ◽

Peikun Xin ◽

...

Keyword(s):

Wound Healing ◽

Ferulic Acid ◽

Natural Antioxidants ◽

Wound Dressings ◽

Polyethylene Glycol Diacrylate ◽

Glycol Diacrylate ◽

Antioxidant Function ◽

Healing Tissue ◽

Anti Oxidant ◽

Gel Network

Abstract Background During wound healing, the overproduction of reactive oxygen species (ROS) can break the cellular oxidant/antioxidant balance, which prolongs healing. The wound dressings targeting the mitigation of ROS will be of great advantages for the wound healing. puerarin (PUE) and ferulic acid (FA) are natural compounds derived from herbs that exhibit multiple pharmacological activities, such as antioxidant and anti-inflammatory effects. Polydopamine (PDA) is made from natural dopamine and shows excellent antioxidant function. Therefore, the combination of natural antioxidants into hydrogel dressing is a promising therapy for wound healing. Results Hydrogel wound dressings have been developed by incorporating PUE or FA via PDA nanoparticles (NPs) into polyethylene glycol diacrylate (PEG-DA) hydrogel. This hydrogel can load natural antioxidant drugs and retain the drug in the gel network for a long period due to the presence of PDA NPs. Under oxidative stress, this hydrogel can improve the activity of superoxide dismutase and glutathione peroxidase and reduce the levels of ROS and malondialdehyde, thus preventing oxidative damage to cells, and then promoting wound healing, tissue regeneration, and collagen accumulation. Conclusion Overall, this triple antioxidant hydrogel accelerates wound healing by alleviating oxidative injury. Our study thus provides a new way about co-delivery of multiple antioxidant natural molecules from herbs via antioxidant nanoparticles for wound healing and skin regeneration. Graphic Abstract

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3D bioprinting of photo‐crosslinkable silk methacrylate ( SilMA )‐polyethylene glycol diacrylate ( PEGDA ) bioink for cartilage tissue engineering

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.37336 ◽

2021 ◽

Author(s):

Ashutosh Bandyopadhyay ◽

Biman B. Mandal ◽

Nandana Bhardwaj

Keyword(s):

Tissue Engineering ◽

Polyethylene Glycol ◽

Cartilage Tissue Engineering ◽

Cartilage Tissue ◽

3D Bioprinting ◽

Polyethylene Glycol Diacrylate ◽

Glycol Diacrylate

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Synthesis and Characterization of Poly[N-Vinyl-2-Pyrrolidone-Polyethylene Glycol Diacrylate] Copolymeric Hydrogels for Drug Delivery

Journal of Macromolecular Science Part A ◽

10.1080/10601329708010060 ◽

1997 ◽

Vol 34 (12) ◽

pp. 2461-2470 ◽

Cited By ~ 2

Author(s):

C. Yamini ◽

K. L. Shantha ◽

K. Panduranga Rao

Keyword(s):

Drug Delivery ◽

Polyethylene Glycol ◽

Synthesis And Characterization ◽

Polyethylene Glycol Diacrylate ◽

Glycol Diacrylate

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Polyacryloyl hydrazide based injectable & stimuli responsive hydrogels with tunable properties

Journal of Materials Chemistry B ◽

10.1039/c4tb01257g ◽

2014 ◽

Vol 2 (42) ◽

pp. 7429-7439 ◽

Cited By ~ 4

Author(s):

Anuj Kumar ◽

Sabindra K. Samal ◽

Rupesh Dash ◽

Umaprasana Ojha

Keyword(s):

Polyethylene Glycol ◽

Acrylic Acid ◽

Diethyl Malonate ◽

Synthesis And Characterization ◽

Cross Linking ◽

Stimuli Responsive ◽

Polyethylene Glycol Diacrylate ◽

Glycol Diacrylate ◽

Responsive Hydrogels

The synthesis and characterization of a series of injectable and stimuli responsive hydrogels based on polyacryloyl hydrazide have been accomplished using dimethyl 2,2′-thiodiacetate, acrylic acid, diethyl malonate and polyethylene glycol diacrylate as cross-linkers through a chemical or dual cross-linking pathway.

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Fabrication of PEG Hydrogel and PDMS Microstructures by a Simple UV Curing Process for Nanobio-Chip Applications

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.941-944.404 ◽

2014 ◽

Vol 941-944 ◽

pp. 404-410 ◽

Cited By ~ 1

Author(s):

Young Ho Kim ◽

Jeong Woo Sohn ◽

Youngjae Woo ◽

Joo Hyun Hong ◽

Juyoung Park

Keyword(s):

Polyethylene Glycol ◽

Uv Irradiation ◽

Large Scale ◽

Uv Light ◽

Single Step ◽

Polydimethyl Siloxane ◽

Polyethylene Glycol Diacrylate ◽

Glycol Diacrylate ◽

Metal Mask ◽

Glass Chip

Polyethylene glycol (PEG) hydrogel microstructures with various shapes and sizes on a glass chip were prepared by a simple and rapid ultraviolet (UV) irradiation method using a metal mask. Photocurable PEG solution prepared by mixing 95 wt.% polyethylene glycol diacrylate and 5 wt.% 2-hydroxy-2-methylpropiophenone as a photo-initiator was injected to the gap between bottom and upper glasses in a simply assembled glass chip. After a metal mask with line-and-space or complex patterns was placed on the glass chip, UV light from a spot UV irradiation device was exposed to the glass chip through the metal mask for 7 seconds at UV intensity of 26 mW/cm2. Then the PEG hydrogel micropatterns on the glass chip were obtained after removing unreacted PEG solution by air blowing. To prepare more rigid microstructure, the prepared PEG micropatterned chip was exposed under UV light for 20 seconds. Then the PEG hydrogel micropattern chip was fabricated by a simple and rapid procedure. Micropattern transferring was performed from the PEG hydrogel chip to polydimethyl siloxane (PDMS) replica by a solution casting. The prepared micropatterned PDMS replicas showed similar shape and size of microstructures compared to that of the corresponded PEG hydrogel chip. Thus the PEG hydrogel microstructures on a glass chip could be used as a mold to fabricate micropattern PDMS chips for nanobio-chip applications. Furthermore, the present method provides large scale chip fabrication, more than 4 cm-length and 4 cm-width in a single step, not only PEG hydrogel chips but also PDMS chips.

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