Distance-Based Paper Device for a Naked-Eye Albumin-to-Alkaline Phosphatase Ratio Assay

ACS Sensors ◽  
2021 ◽  
Author(s):  
Kamonchanok Phoonsawat ◽  
Kawin Khachornsakkul ◽  
Nalin Ratnarathorn ◽  
Charles S. Henry ◽  
Wijitar Dungchai
Keyword(s):  
Alkaline Phosphatase ◽  
Naked Eye ◽  
Paper Device

Naked-eye sensitive detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) based on a horseradish peroxidase catalytic colorimetric system with Cu(ii)

The Analyst ◽  
2016 ◽  
Vol 141 (19) ◽  
pp. 5549-5554 ◽  
Author(s):  
Dongmin Shi ◽  
Yue Sun ◽  
Lin Lin ◽  
Chunjun Shi ◽  
Guangfeng Wang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Horseradish Peroxidase ◽  
Colorimetric Method ◽  
Sensitive Detection ◽  
Naked Eye ◽  
Colorimetric System ◽  
System P

In this paper, a novel colorimetric method for the detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) was designed based on a Cu2+–horseradish peroxidase (HRP)–3,3′,5,5′-tetra-methylbenzidine (TMB)–H2O2 system.

Naked-eye quantitative aptamer-based assay on paper device

2016 ◽  
Vol 78 ◽  
pp. 538-546 ◽  
Author(s):  
Yun Zhang ◽  
Dong Gao ◽  
Jinlong Fan ◽  
Jinfang Nie ◽  
Shangwang Le ◽  
...  
Keyword(s):  
Naked Eye ◽  
Paper Device

scholarly journals Distance-Based Paper Device Combined with Headspace Extraction for Determination of Cyanide

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2340 ◽  
Author(s):  
Papichaya Khatha ◽  
Thanyarat Phutthaphongloet ◽  
Phenphitcha Timpa ◽  
Benjawan Ninwong ◽  
Kamolwich Income ◽  
...  
Keyword(s):  
Drinking Water ◽  
Tap Water ◽  
World Health ◽  
Ag Nps ◽  
Imaging Device ◽  
Naked Eye ◽  
Headspace Extraction ◽  
Gold Silver ◽  
Paper Device

We report for the first time a distance-based paper device based on gold/silver core shell nanoparticles (Au@Ag NPs) for a simple, inexpensive, instrument-free, and portable determination of cyanide by the naked eye. Au@Ag NPs immobilized on a paper channel were etched by cyanide ions so that a yellow color band length of Au@Ag NPs is proportional to a decrease in the cyanide concentration. Quantification is achieved by measuring color length, thus eliminating the need to differentiate hues and intensities by the user, and the processing data of each imaging device. Moreover, the paper-based headspace extraction was combined with the distance-based paper device to improve the sensitivity. The enrichment factor was found to be 30-fold and the linearity was found in the range 0.05–1 mg L−1. The naked eye detection limit was 10 μg L−1 where the World Health Organization (WHO) have regulated the maximum level of cyanide in drinking water as 70 μg L−1. Our proposed device also showed no interference from common cations and anions presenting in seawater and waste water including thiocyanate, chloride. Finally, our device has been successfully applied to determine cyanide ions in seawater, drinking water, tap water and wastewater providing satisfactory precision and accuracy.

scholarly journals DNA–Gold Nanozyme-Modified Paper Device for Enhanced Colorimetric Detection of Mercury Ions

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 211
Author(s):  
Min-Xin Mao ◽  
Rong Zheng ◽  
Chi-Fang Peng ◽  
Xin-Lin Wei
Keyword(s):  
Low Cost ◽  
Colorimetric Detection ◽  
Environmental Water ◽  
Mercury Ions ◽  
Naked Eye ◽  
Quantitative Measurements ◽  
Color Response ◽  
Paper Chip ◽  
Paper Device ◽  
Optimized Conditions

In this work, a paper device consisted of a patterned paper chip, wicking pads, and a base was fabricated. On the paper chip, DNA–gold nanoparticles (DNA–AuNPs) were deposited and Hg2+ ions could be adsorbed by the DNA–AuNPs. The formed DNA–AuNP/Hg2+ nanozyme could catalyze the tetramethylbenzidine (TMB)–H2O2 chromogenic reaction. Due to the wicking pads, a larger volume of Hg2+ sample could be applied to the paper device for Hg2+ detection and therefore the color response could be enhanced. The paper device achieved a cut-off value of 50 nM by the naked eye for Hg2+ under optimized conditions. Moreover, quantitative measurements could be implemented by using a desktop scanner and extracting grayscale values. A linear range of 50–2000 nM Hg2+ was obtained with a detection limit of 10 nM. In addition, the paper device could be applied in the detection of environmental water samples with high recoveries ranging from 85.7% to 105.6%. The paper-device-based colorimetric detection was low-cost, simple, and demonstrated high potential in real-sample applications.

Naked-eye quantitative assay on paper device for date rape drug sensing via smart phone APP

Vacuum ◽  
2018 ◽  
Vol 153 ◽  
pp. 300-305 ◽  
Author(s):  
Jagriti Narang ◽  
Chaitali Singhal ◽  
Ashish Mathur ◽  
Ashwani Kumar Dubey ◽  
Anoop Krishna PN ◽  
...  
Keyword(s):  
Date Rape ◽  
Smart Phone ◽  
Quantitative Assay ◽  
Naked Eye ◽  
Paper Device ◽  
Date Rape Drug

Rapid naked eye detection of alkaline phosphatase using α-MoO3-x nano-flakes

2018 ◽  
Vol 254 ◽  
pp. 514-518 ◽  
Author(s):  
Sunil Pandey ◽  
Amit Kumar Sharma ◽  
Krishna Hari Sharma ◽  
Yowan Nerthigan ◽  
M. Shahnawaz Khan ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Eye Detection ◽  
Naked Eye ◽  
Naked Eye Detection

Interrelationship of the cellular distribution and secretion of regular structure (RS) protein in Bacillus licheniformis nm 105

1992 ◽  
Vol 50 (1) ◽  
pp. 712-713
Author(s):  
Xiaorong Zhu ◽  
Richard McVeigh ◽  
Bijan K. Ghosh
Keyword(s):  
Alkaline Phosphatase ◽  
Bacillus Licheniformis ◽  
Self Assembly ◽  
Gel Electrophoresis ◽  
Culture Supernatant ◽  
Regular Structure ◽  
The Self ◽  
Cellular Distribution ◽  
Structural Protein ◽  
Laboratory Cultures

A mutant of Bacillus licheniformis 749/C, NM 105 exhibits some notable properties, e.g., arrest of alkaline phosphatase secretion and overexpression and hypersecretion of RS protein. Although RS is known to be widely distributed in many microbes, it is rarely found, with a few exceptions, in laboratory cultures of microorganisms. RS protein is a structural protein and has the unusual properties to form aggregate. This characteristic may have been responsible for the self assembly of RS into regular tetragonal structures. Another uncommon characteristic of RS is that enhanced synthesis and secretion which occurs when the cells cease to grow. Assembled RS protein with a tetragonal structure is not seen inside cells at any stage of cell growth including cells in the stationary phase of growth. Gel electrophoresis of the culture supernatant shows a very large amount of RS protein in the stationary culture of the B. licheniformis. It seems, Therefore, that the RS protein is cotranslationally secreted and self assembled on the envelope surface.

In Situ Localization of PPAR Gamma and Uncoupling Protein in Mouse Embryo Sections Using Digoxigenin-Labeled Riboprobes

1996 ◽  
Vol 54 ◽  
pp. 32-33
Author(s):  
C. Jennermann ◽  
S. A. Kliewer ◽  
D. C. Morris
Keyword(s):  
Alkaline Phosphatase ◽  
Room Temperature ◽  
Uncoupling Protein ◽  
Ppar Gamma ◽  
Nuclear Hormone Receptor ◽  
Full Length ◽  
Northern Analysis ◽  
Peroxisome Proliferator

Peroxisome proliferator-activated receptor gamma (PPARg) is a member of the nuclear hormone receptor superfamily and has been shown in vitro to regulate genes involved in lipid metabolism and adipocyte differentiation. By Northern analysis, we and other researchers have shown that expression of this receptor predominates in adipose tissue in adult mice, and appears first in whole-embryo mRNA at 13.5 days postconception. In situ hybridization was used to find out in which developing tissues PPARg is specifically expressed.Digoxigenin-labeled riboprobes were generated using the Genius™ 4 RNA Labeling Kit from Boehringer Mannheim. Full length PPAR gamma, obtained by PCR from mouse liver cDNA, was inserted into pBluescript SK and used as template for the transcription reaction. Probes of average size 200 base pairs were made by partial alkaline hydrolysis of the full length transcripts. The in situ hybridization assays were performed as described previously with some modifications. Frozen sections (10 μm thick) of day 18 mouse embryos were cut, fixed with 4% paraformaldehyde and acetylated with 0.25% acetic anhydride in 1.0M triethanolamine buffer. The sections were incubated for 2 hours at room temperature in pre-hybridization buffer, and were then hybridized with a probe concentration of 200μg per ml at 70° C, overnight in a humidified chamber. Following stringent washes in SSC buffers, the immunological detection steps were performed at room temperature. The alkaline phosphatase labeled, anti-digoxigenin antibody and detection buffers were purchased from Boehringer Mannheim. The sections were treated with a blocking buffer for one hour and incubated with antibody solution at a 1:5000 dilution for 2 hours, both at room temperature. Colored precipitate was formed by exposure to the alkaline phosphatase substrate nitrobluetetrazoliumchloride/ bromo-chloroindlylphosphate.

Electron microscopic study of the membrane glycoproteins in the rat kidney after cisplatin treatment

1989 ◽  
Vol 47 ◽  
pp. 912-913
Author(s):  
S.K. Aggarwal
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Kidney ◽  
Light And Electron Microscopy ◽  
Kidney Tissue ◽  
Membrane Glycoproteins ◽  
Electron Microscopic ◽  
Before And After ◽  
Interstrand Cross Links ◽  
Cross Links

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

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