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Talanta ◽  
2022 ◽  
Vol 236 ◽  
pp. 122858
Author(s):  
Pavar Sai Kumar ◽  
Sunil Bhand ◽  
Ashis Kumar Das ◽  
Sanket Goel
Keyword(s):  

Author(s):  
Sursak Kasetsirikul ◽  
Kimberley Clack ◽  
Muhammad J.A. Shiddiky ◽  
Nam-Trung Nguyen

Paper-based analytical devices have been substantially developed in recent decades. Many fabrication techniques for paper-based analytical devices have been demonstrated and reported. Herein we report a relatively rapid, simple, and inexpensive method for fabricating paper-based analytical devices using parafilm hot pressing. We studied and optimized the effect of the key fabrication parameters, namely pressure, temperature, and pressing time. We discerned the optimal conditions, including pressure of 3.8 MPa (3 tons), temperature of 80oC, and 3 minutes of pressing time, with the smallest hydrophobic barrier size (821 µm) being governed by laminate mask and parafilm dispersal from pressure and heat. Physical and biochemical properties were evaluated to substantiate the paper functionality for analytical devices. Wicking speed in the fabricated paper strips was slightly slower than that of non-processed paper, resulting from reducing paper pore size. A colorimetric immunological assay was performed to demonstrate the protein binding capacity of the paper-based device after exposure to pressure and heat from the fabrication. Moreover, mixing in two-dimensional paper-based device and flowing in a three-dimensional counterpart were thoroughly investigated, demonstrating that the paper device from this fabrication process is potentially applicable as analytical devices for biomolecule detection. Fast, easy, and inexpensive parafilm hot press fabrication presents an opportunity for researchers to develop paper-based analytical devices in resource-limited environments.


2021 ◽  
Vol 208 ◽  
pp. 112056
Author(s):  
Jaligam Murali Mohan ◽  
Khairunnisa Amreen ◽  
Madhusudan B. Kulkarni ◽  
Arshad Javed ◽  
Satish Kumar Dubey ◽  
...  
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2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Emily C. Kight ◽  
Iftak Hussain ◽  
Audrey K. Bowden ◽  
Frederick R. Haselton

AbstractOvarian cancer has a poor cure rate and rates of relapse are high. Current recurrence detection is limited by non-specific methods such as blood testing and ultrasound. Based on reports that human epididymis four (HE4) / creatinine (CRE) ratios found in urine are elevated in ovarian cancers, we have developed a paper-based device that combines lateral flow technology and cell phone analysis to quantitatively measure HE4/CRE. Surrogate samples were used to test the performance over clinically expected HE4/CRE ratios. For HE4/CRE ratios of 2 to 47, the percent error was found to be 16.0% on average whether measured by a flatbed scanner or cell phone. There was not a significant difference between the results from the cell phone or scanner. Based on published studies, error in this method was less than the difference required to detect recurrence. This promising new tool, with further development, could be used at home or in low-resource settings to provide timely detection of ovarian cancer recurrence.


2021 ◽  
Author(s):  
E. Coz ◽  
P. Garneret ◽  
E. Martin ◽  
D. F. do Nascimento ◽  
A. Vilquin ◽  
...  

ABSTRACTObjectivesDetermine the sensitivity and specificity of a Point-Of-Care test (‘COVIDISC’) for SARS-COV2. The novelty of the test is to integrate, on the same (low-cost) compact plastic/paper device, solid phase RNA extraction and RT-LAMP amplification, all reagents being freeze-dried on it.MethodRetrospective study with a cohort of 99 patients characterized by real-time RT-PCR. The 37 positive naso-pharyngeal samples cover a broad range of viral loads (from 5 gc /µL to 2 106 gc/ µL of sample).ResultsThe COVIDISC found 36 positives (out of 37 by IP4 RT-PCR protocols) and 63 negatives (out of 62 by RT-PCR).ConclusionThe sensitivity of the COVIDISC, found in this 99-patient retrospective study, is 97% and the specificity 100%.


Crystals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1053
Author(s):  
Vladimir G. Chigrinov ◽  
Aleksey A. Kudreyko ◽  
Fedor V. Podgornov

Recent achievements in the photoalignment technique for fabrication of optically rewritable electronic paper with high performance characteristics are surveyed with emphasis on temporal constraints on the exposure process. The possibility of creating electrode-free electronic paper has very important practical aspects. However, many existing studies do not include sufficient analysis on how to achieve acceptable reflective characteristics within short exposure time. In order to achieve this goal, we have applied the rotational diffusion model. We find that the parameters of the diffusion model can be adjusted to get acceptable light-reflecting characteristics within 10 s of exposure. In comparison with the long-time exposure, the reflectance coefficient reduces by 24%. The route to material improvements for optimized e-paper device is discussed.


ACS Sensors ◽  
2021 ◽  
Author(s):  
Kamonchanok Phoonsawat ◽  
Kawin Khachornsakkul ◽  
Nalin Ratnarathorn ◽  
Charles S. Henry ◽  
Wijitar Dungchai

Author(s):  
Mathews de Oliveira Krambeck Franco ◽  
Willian Toito Suarez ◽  
Vagner Bezerra dos Santos ◽  
Ian Santana Resque ◽  
Marcelo Henrique dos Santos ◽  
...  
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