scholarly journals Cell-Free Gene Expression Dynamics in Synthetic Cell Populations

2022 ◽  
Author(s):  
David T. Gonzales ◽  
Naresh Yandrapalli ◽  
Tom Robinson ◽  
Christoph Zechner ◽  
T-Y. Dora Tang
Keyword(s):  
Gene Expression ◽  
Cell Populations ◽  
Synthetic Cell ◽  
Gene Expression Dynamics ◽  
Free Gene

scholarly journals Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jasmine M. Hershewe ◽  
Katherine F. Warfel ◽  
Shaelyn M. Iyer ◽  
Justin A. Peruzzi ◽  
Claretta J. Sullivan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Synthetic Biology ◽  
Membrane Protein ◽  
Membrane Vesicles ◽  
Processing Methods ◽  
Glycoprotein Synthesis ◽  
On Demand ◽  
Free Gene ◽  
Membrane Bound

AbstractCell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been limited. Here, we address this limitation by characterizing native membrane vesicles in Escherichia coli-based CFE extracts and describing methods to enrich vesicles with heterologous, membrane-bound machinery. As a model, we focus on bacterial glycoengineering. We first use multiple, orthogonal techniques to characterize vesicles and show how extract processing methods can be used to increase concentrations of membrane vesicles in CFE systems. Then, we show that extracts enriched in vesicle number also display enhanced concentrations of heterologous membrane protein cargo. Finally, we apply our methods to enrich membrane-bound oligosaccharyltransferases and lipid-linked oligosaccharides for improving cell-free N-linked and O-linked glycoprotein synthesis. We anticipate that these methods will facilitate on-demand glycoprotein production and enable new CFE systems with membrane-associated activities.

scholarly journals Gene expression dynamics underlying cell fate emergence in 2D micropatterned human embryonic stem cell gastruloids

2021 ◽  
Author(s):  
Kyaw Thu Minn ◽  
Sabine Dietmann ◽  
Sarah E. Waye ◽  
Samantha A. Morris ◽  
Lilianna Solnica-Krezel
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Fate ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Gene Expression Dynamics

Aleukemic granulocytic sarcoma with AML1/ETO fusion gene expression and clonal T cell populations

2001 ◽  
Vol 25 (12) ◽  
pp. 1137-1142 ◽  
Author(s):  
Jiřı́ Schwarz ◽  
Zuzana Trnková ◽  
Renáta Bedrlı́ková ◽  
Adam Jirásek ◽  
Dana Žáková ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
Fusion Gene ◽  
Granulocytic Sarcoma ◽  
Cell Populations

scholarly journals Characterization and Regulation of Type A Endothelin Receptor Gene Expression in Bovine Luteal Cell Types

Endocrinology ◽  
1999 ◽  
Vol 140 (5) ◽  
pp. 2110-2116 ◽  
Author(s):  
Roni Mamluk ◽  
Nitzan Levy ◽  
Bo Rueda ◽  
John S. Davis ◽  
Rina Meidan
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Cell Types ◽  
Cell Populations ◽  
Mrna Levels ◽  
Factor I ◽  
Receptor Mrna ◽  
Progesterone Production ◽  
Eta Receptor ◽  
Receptor Gene Expression

Abstract Our previous studies demonstrated that endothelin-1 (ET-1), a 21-amino acid vasoconstrictor peptide, has a paracrine regulatory role in bovine corpus luteum (CL). The peptide is produced within the gland where it inhibits progesterone production by acting via the selective type A endothelin (ETA) receptors. The present study was designed to characterize ETA receptor gene expression in different ovarian cell types and its hormonal regulation. ETA receptor messenger RNA (mRNA) levels were high in follicular cells as well as in CL during luteal regression. At this latter stage, high ETA receptor expression concurred with low prostaglandin F2α receptor mRNA. The ETA receptor gene was expressed by all three major cell populations of the bovine CL; i.e. small and large luteal cells, as well as in luteal endothelial cells. Among these various cell populations, the highest ETA receptor mRNA levels were found in endothelial cells. cAMP elevating agents, forskolin and LH, suppressed ETA receptor mRNA expression in luteinized theca cells (LTC). This inhibition was dose dependent and was evident already after 24 h of incubation. In luteinized granulosa cells (LGC), 10 and 100 ng/ml of insulin-like growth factor I and insulin (only at a concentration of 2000 ng/ml) markedly decreased ETA receptor mRNA levels. In both LGC and LTC there was an inverse relationship between ETA receptor gene expression and progesterone production; insulin (in LGC) and forskolin (in LTC) enhanced progesterone production while inhibiting ETA receptor mRNA levels. Our findings may therefore suggest that, during early stages of luteinization when peak levels of both LH and insulin-like growth factor I exist, the expression of ETA receptors in the gland are suppressed. This study demonstrates physiologically relevant regulatory mechanisms controlling ETA receptor gene expression and further supports the inhibitory role of ET-1 in CL function.

scholarly journals Genome‐wide gene expression dynamics of the fungal pathogen D othistroma septosporum throughout its infection cycle of the gymnosperm host P inus radiata

2015 ◽  
Vol 17 (2) ◽  
pp. 210-224 ◽  
Author(s):  
Rosie E. Bradshaw ◽  
Yanan Guo ◽  
Andre D. Sim ◽  
M. Shahjahan Kabir ◽  
Pranav Chettri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fungal Pathogen ◽  
Infection Cycle ◽  
Genome Wide ◽  
Gene Expression Dynamics ◽  
Genome Wide Gene Expression
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