Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei

2021 ◽  
Author(s):  
Shunxing Chai ◽  
Zhihua Zhu ◽  
Ernuo Tian ◽  
Meili Xiao ◽  
Yan Wang ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Protein Production ◽  
Production Platform

scholarly journals Metabolic engineering of CHO cells for the development of a robust protein production platform

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0181455 ◽  
Author(s):  
Sanjeev Kumar Gupta ◽  
Santosh K. Srivastava ◽  
Ankit Sharma ◽  
Vaibhav H. H. Nalage ◽  
Darshita Salvi ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Cho Cells ◽  
Protein Production ◽  
Production Platform

scholarly journals Erratum: Ntana, F., et al. Aspergillus: A Powerful Protein Production Platform. Catalysts 2020, 10, 1064

Catalysts ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1400
Author(s):  
Fani Ntana ◽  
Uffe Hasbro Mortensen ◽  
Catherine Sarazin ◽  
Rainer Figge
Keyword(s):  
Protein Production ◽  
Production Platform

The author wishes to make the following erratum to this paper [...]

The effect of specific growth rate on protein synthesis and secretion in the filamentous fungus Trichoderma reesei

Microbiology ◽  
2005 ◽  
Vol 151 (1) ◽  
pp. 135-143 ◽  
Author(s):  
Tiina M. Pakula ◽  
Katri Salonen ◽  
Jaana Uusitalo ◽  
Merja Penttilä
Keyword(s):  
Growth Rate ◽  
Protein Synthesis ◽  
Specific Growth Rate ◽  
Trichoderma Reesei ◽  
Growth Rates ◽  
Protein Production ◽  
Specific Growth ◽  
Synthesis Rate ◽  
Specific Rate ◽  
Specific Growth Rates

Trichoderma reesei was cultivated in chemostat cultures on lactose-containing medium. The cultures were characterized for growth, consumption of the carbon source and protein production. Secreted proteins were produced most efficiently at low specific growth rates, 0·022–0·033 h−1, the highest specific rate of total protein production being 4·1 mg g−1 h−1 at the specific growth rate 0·031 h−1. At low specific growth rates, up to 29 % of the proteins produced were extracellular, in comparison to only 6–8 % at high specific growth rates, 0·045–0·066 h−1. To analyse protein synthesis and secretion in more detail, metabolic labelling of proteins was applied to analyse production of the major secreted protein, cellobiohydrolase I (CBHI, Cel7A). Intracellular and extracellular labelled CBHI was quantified and analysed for pI isoforms in two-dimensional gels, and the synthesis and secretion rates of the molecule were determined. Both the specific rates of CBHI synthesis and secretion were highest at low specific growth rates, the optimum being at 0·031 h−1. However, at low specific growth rates the secretion rate/synthesis rate ratio was significantly lower than that at high specific growth rates, indicating that at low growth rates the capacity of cells to transport the protein becomes limiting. In accordance with the high level of protein production and limitation in the secretory capacity, the transcript levels of the unfolded protein response (UPR) target genes pdi1 and bip1 as well as the gene encoding the UPR transcription factor hac1 were induced.

107 Bioactive chicken IL-12: Plant-derived protein production platform compatible with veterinary vaccine applications

Cytokine ◽  
2008 ◽  
Vol 43 (3) ◽  
pp. 260-261
Author(s):  
Giuliana Medrano ◽  
Nathan Stephans ◽  
David Radin ◽  
Maureen C. Dolan ◽  
Carole L. Cramer
Keyword(s):  
Protein Production ◽  
Veterinary Vaccine ◽  
Production Platform

scholarly journals Novel genetic tools that enable highly pure protein production in Trichoderma reesei

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anssi Rantasalo ◽  
Marika Vitikainen ◽  
Toni Paasikallio ◽  
Jussi Jäntti ◽  
Christopher P. Landowski ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Protein Production ◽  
Genetic Tools ◽  
Pure Protein

Influence of sorbitol on protein production and glycosylation and cell wall formation in Trichoderma reesei

2010 ◽  
Vol 114 (10) ◽  
pp. 855-862 ◽  
Author(s):  
Wioletta Górka-Nieć ◽  
Urszula Perlińska-Lenart ◽  
Patrycja Zembek ◽  
Grażyna Palamarczyk ◽  
Joanna S. Kruszewska
Keyword(s):  
Cell Wall ◽  
Trichoderma Reesei ◽  
Protein Production ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Modification of transcriptional factor ACE3 enhances protein production in Trichoderma reesei in the absence of cellulase gene inducer

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yun Luo ◽  
Mari Valkonen ◽  
Raymond E. Jackson ◽  
Jonathan M. Palmer ◽  
Aditya Bhalla ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Protein Production ◽  
Transcriptional Factor ◽  
Cellulase Gene
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