Improvement of Stability and Cell Adhesion Properties of Polyelectrolyte Multilayer Films by Chemical Cross-Linking

AbstractThe seeding of endothelial cells on biomaterial surfaces has become a major challenge to achieve better haemocompatibility of these surfaces. Multilayers of polyelectrolytes formed by the layerby-layer method are promising in this respect. In this study, the interactions of endothelial cells with multilayered polyelectrolytes films were investigated. The build-ups were prepared by selfassembled alternatively adsorbed polyanions and polycations functionalised with fibronectin and collagen. Anionic poly(sodium 4-styrenesulfonate) and cationic poly(allylamine hydrochloride) polyelectrolytes were chosen as a model system. Elaborated surfaces were characterised by electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode showed good reversible electrochemical properties and high stability in an electrolyte solution. The film ohmic resistance was highest when the film was coated with fibronectin; the parameters so determined were correlated with atomic force microscopy images. Cell colorimetric assay (WST-1) and immunofluorescence were used to quantify the cell viability and evaluate the adhesion properties. When cultured on a surface where proteins were deposited, cells adhered and proliferated better with fibronectin than with collagen. In addition, a high surface free energy was favourable to adhesion and proliferation (48.8 mJ m−2 for fibronectin and 39.7 mJ m−2 for collagen, respectively). Endothelial cells seeded on functionalised-polyelectrolyte multilayer films showed a good morphology and adhesion necessary for the development of a new endothelium.

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pH dependent growth of poly(L-lysine)/poly(L-glutamic) acid multilayer films and their cell adhesion properties

Surface Science ◽

10.1016/j.susc.2004.06.178 ◽

2004 ◽

Vol 570 (1-2) ◽

pp. 13-29 ◽

Cited By ~ 114

Author(s):

Ludovic Richert ◽

Youri Arntz ◽

Pierre Schaaf ◽

Jean-Claude Voegel ◽

Catherine Picart

Keyword(s):

Cell Adhesion ◽

Glutamic Acid ◽

Multilayer Films ◽

Adhesion Properties ◽

Ph Dependent ◽

Dependent Growth

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Facile fabrication of robust multilayer films: visible light-triggered chemical cross-linking by the catalysis of a ruthenium(ii) complex

Chemical Communications ◽

10.1039/c0cc03713c ◽

2011 ◽

Vol 47 (3) ◽

pp. 929-931 ◽

Cited By ~ 16

Author(s):

You Yu ◽

Hui Zhang ◽

Caihong Zhang ◽

Shuxun Cui

Keyword(s):

Visible Light ◽

Multilayer Films ◽

Cross Linking ◽

Facile Fabrication ◽

Chemical Cross Linking

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Cross-linking multilayers of poly-l-lysine and hyaluronic acid: Effect on mesenchymal stem cell behavior

The International Journal of Artificial Organs ◽

10.1177/0391398817752598 ◽

2018 ◽

Vol 41 (4) ◽

pp. 223-235 ◽

Cited By ~ 11

Author(s):

Marcus S Niepel ◽

Fadi Almouhanna ◽

Bhavya K Ekambaram ◽

Matthias Menzel ◽

Andreas Heilmann ◽

...

Keyword(s):

Stem Cell ◽

Hyaluronic Acid ◽

Adipogenic Differentiation ◽

Focal Adhesions ◽

Stem Cell Differentiation ◽

Multilayer System ◽

Polyelectrolyte Multilayers ◽

Cross Linking ◽

Polyelectrolyte Multilayer ◽

Chemical Cross Linking

Background: Cells possess a specialized machinery through which they can sense physical as well as chemical alterations in their surrounding microenvironment that affect their cellular behavior. Aim: In this study, we aim to establish a polyelectrolyte multilayer system of 24 layers of poly-l-lysine and hyaluronic acid to control stem cell response after chemical cross-linking. Methods and results: The multilayer build-up process is monitored using different methods, which show that the studied polyelectrolyte multilayer system grows exponentially following the islands and islets theory. Successful chemical cross-linking is monitored by an increased zeta potential toward negative magnitude and an extraordinary growth in thickness. Human adipose–derived stem cells are used here and a relationship between cross-linking degree and cell spreading is shown as cells seeded on higher cross-linked polyelectrolyte multilayer show enhanced spreading. Furthermore, cells that fail to establish focal adhesions on native and low cross-linked polyelectrolyte multilayer films do not proliferate to a high extent in comparison to cells seeded on highly cross-linked polyelectrolyte multilayer, which also show an increased metabolic activity. Moreover, this study shows the relation between cross-linking degree and human adipose–derived stem cell lineage commitment. Histological staining reveals that highly cross-linked polyelectrolyte multilayers support osteogenic differentiation, whereas less cross-linked and native polyelectrolyte multilayers support adipogenic differentiation in the absence of any specific inducers. Conclusion: Owing to the precise control of polyelectrolyte multilayer properties such as potential, wettability, and viscoelasticity, the system presented here offers great potential for guided stem cell differentiation in regenerative medicine, especially in combination with materials exhibiting a defined surface topography.

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Polyelectrolyte Multilayer Films Modification with Ag and rGO Influences Platelets Activation and Aggregate Formation under In Vitro Blood Flow

Nanomaterials ◽

10.3390/nano10050859 ◽

2020 ◽

Vol 10 (5) ◽

pp. 859 ◽

Cited By ~ 1

Author(s):

Gabriela Imbir ◽

Aldona Mzyk ◽

Klaudia Trembecka-Wójciga ◽

Ewa Jasek-Gajda ◽

Hanna Plutecka ◽

...

Keyword(s):

Plasma Proteins ◽

Multilayer Films ◽

Chemical Parameters ◽

Polyelectrolyte Multilayer ◽

Physico Chemical ◽

Substrate Properties ◽

Promising Solution ◽

Blood Plasma Proteins ◽

Chemical Cross Linking

Surface functionalization of materials to improve their hemocompatibility is a challenging problem in the field of blood-contacting devices and implants. Polyelectrolyte multilayer films (PEMs), which can mimic functions and structure of an extracellular matrix (ECM), are a promising solution to the urgent need for functional blood-contacting coatings. The properties of PEMs can be easily tuned in order to provide a scaffold with desired physico-chemical parameters. In this study chitosan/chondroitin sulfate (Chi/CS) polyelectrolyte multilayers were deposited on medical polyurethane. Afterwards PEMs were modified by chemical cross-linking and nanoparticles introduction. Coatings with variable properties were tested for their hemocompatibility in the cone-plate tester under dynamic conditions. The obtained results enable the understanding of how substrate properties modulate PEMs interaction with blood plasma proteins and the morphotic elements.

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