Cross-linking multilayers of poly-l-lysine and hyaluronic acid: Effect on mesenchymal stem cell behavior

2018 ◽  
Vol 41 (4) ◽  
pp. 223-235 ◽  
Author(s):  
Marcus S Niepel ◽  
Fadi Almouhanna ◽  
Bhavya K Ekambaram ◽  
Matthias Menzel ◽  
Andreas Heilmann ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Adipogenic Differentiation ◽  
Focal Adhesions ◽  
Stem Cell Differentiation ◽  
Multilayer System ◽  
Polyelectrolyte Multilayers ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer ◽  
Chemical Cross Linking

Background: Cells possess a specialized machinery through which they can sense physical as well as chemical alterations in their surrounding microenvironment that affect their cellular behavior. Aim: In this study, we aim to establish a polyelectrolyte multilayer system of 24 layers of poly-l-lysine and hyaluronic acid to control stem cell response after chemical cross-linking. Methods and results: The multilayer build-up process is monitored using different methods, which show that the studied polyelectrolyte multilayer system grows exponentially following the islands and islets theory. Successful chemical cross-linking is monitored by an increased zeta potential toward negative magnitude and an extraordinary growth in thickness. Human adipose–derived stem cells are used here and a relationship between cross-linking degree and cell spreading is shown as cells seeded on higher cross-linked polyelectrolyte multilayer show enhanced spreading. Furthermore, cells that fail to establish focal adhesions on native and low cross-linked polyelectrolyte multilayer films do not proliferate to a high extent in comparison to cells seeded on highly cross-linked polyelectrolyte multilayer, which also show an increased metabolic activity. Moreover, this study shows the relation between cross-linking degree and human adipose–derived stem cell lineage commitment. Histological staining reveals that highly cross-linked polyelectrolyte multilayers support osteogenic differentiation, whereas less cross-linked and native polyelectrolyte multilayers support adipogenic differentiation in the absence of any specific inducers. Conclusion: Owing to the precise control of polyelectrolyte multilayer properties such as potential, wettability, and viscoelasticity, the system presented here offers great potential for guided stem cell differentiation in regenerative medicine, especially in combination with materials exhibiting a defined surface topography.

Effect of metal ions on physical properties of multilayers from hyaluronan and chitosan and adhesion, growth and adipogenic differentiation of multipotent mouse fi-broblasts

Soft Matter ◽  
2021 ◽  
Author(s):  
Husnia Muftah Kindi ◽  
Matthias Menzel ◽  
Andreas Heilmann ◽  
Christian EH Schmelzer ◽  
Martin Herzberg ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Physical Properties ◽  
Metal Ions ◽  
Adipogenic Differentiation ◽  
Polyelectrolyte Multilayers ◽  
Layer By Layer ◽  
Layer Technique ◽  
Layer By Layer Technique

Polyelectrolyte multilayers (PEM) consisting of the polysaccharides hyaluronic acid (HA) as polyanion and chitosan (Chi) as polycation are prepared by the layer-by-layer technique (LbL). The [Chi/HA]5 multilayers are exposed to...

scholarly journals Cholesterol-Dependent Modulation of Stem Cell Biomechanics: Application to Adipogenesis

2019 ◽  
Vol 141 (8) ◽  
Author(s):  
Shan Sun ◽  
Djanybek Adyshev ◽  
Steven Dudek ◽  
Amit Paul ◽  
Andrew McColloch ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Young’S Modulus ◽  
Cell Mechanics ◽  
Young's Modulus ◽  
Adipogenic Differentiation ◽  
Stem Cell Differentiation ◽  
Cell Stiffness ◽  
Cholesterol Depletion

Cell mechanics has been shown to regulate stem cell differentiation. We have previously reported that altered cell stiffness of mesenchymal stem cells can delay or facilitate biochemically directed differentiation. One of the factors that can affect the cell stiffness is cholesterol. However, the effect of cholesterol on differentiation of human mesenchymal stem cells remains elusive. In this paper, we demonstrate that cholesterol is involved in the modulation of the cell stiffness and subsequent adipogenic differentiation. Rapid cytoskeletal actin reorganization was evident and correlated with the cell's Young's modulus measured using atomic force microscopy. In addition, the level of membrane-bound cholesterol was found to increase during adipogenic differentiation and inversely varied with the cell stiffness. Furthermore, cholesterol played a key role in the regulation of the cell morphology and biomechanics, suggesting its crucial involvement in mechanotransduction. To better understand the underlying mechanisms, we investigated the effect of cholesterol on the membrane–cytoskeleton linker proteins (ezrin and moesin). Cholesterol depletion was found to upregulate the ezrin expression which promoted cell spreading, increased Young's modulus, and hindered adipogenesis. In contrast, cholesterol enrichment increased the moesin expression, decreased Young's modulus, and induced cell rounding and facilitated adipogenesis. Taken together, cholesterol appears to regulate the stem cell mechanics and adipogenesis through the membrane-associated linker proteins.

Effect of Chemical Cross-linking on Properties of Gelatin/Hyaluronic Acid Composite Hydrogels

2013 ◽  
Vol 52 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Zhihua Zhou ◽  
Zhongmin Yang ◽  
Tianlong Huang ◽  
Lihua Liu ◽  
Qingquan Liu ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cross Linking ◽  
Composite Hydrogels ◽  
Chemical Cross Linking

scholarly journals The Nanoscale Geometrical Maturation of Focal Adhesions Controls Stem Cell Differentiation and Mechanotransduction

Nano Letters ◽  
2014 ◽  
Vol 14 (7) ◽  
pp. 3945-3952 ◽  
Author(s):  
Julien E. Gautrot ◽  
Jenny Malmström ◽  
Maria Sundh ◽  
Coert Margadant ◽  
Arnoud Sonnenberg ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Focal Adhesions ◽  
Stem Cell Differentiation

Internal Composition versus the Mechanical Properties of Polyelectrolyte Multilayer Films: The Influence of Chemical Cross-Linking†

Langmuir ◽  
2009 ◽  
Vol 25 (24) ◽  
pp. 13809-13819 ◽  
Author(s):  
Thomas Boudou ◽  
Thomas Crouzier ◽  
Rachel Auzély-Velty ◽  
Karine Glinel ◽  
Catherine Picart
Keyword(s):  
Mechanical Properties ◽  
Multilayer Films ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer ◽  
Chemical Cross Linking

scholarly journals Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Gokhan Duruksu ◽  
Aysegul Aciksari
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Matrix Protein ◽  
Adipogenic Differentiation ◽  
Stem Cell Differentiation ◽  
Natural Polymers ◽  
The Matrix ◽  
Coated Surfaces ◽  
Glycation Process

The microenvironment is an important factor of stem cells regulating their maintenance, survival, and differentiation. The glycation of proteins with reducing sugars through nonenzymatic reactions induces the collagen cross-linking, which causes tissue stiffening, which is enhanced during aging and diabetes. In this study, we aimed to analyze the effect of glycated collagen on the stem cell culture and differentiation. The collagen type 1 was modified by glycation with mannose, rhamnose, arabinose, and glucose. After the culture of mesenchymal stem cells on the coated surfaces with glycated collagen, the differences in cell adhesion, proliferation, and differentiation were compared. The results showed that the modifications did not induce apoptosis or cause cell death. However, the culture of cells on modified collagens improved the proliferation. It was found that the mannose-modified collagen stimulated the adipogenic differentiation of stem cells, and rhamnose-modified collagen supports the differentiation into both osteogenic and insulin-producing cells. The low concentration of monosaccharides during glycation process improved the characteristics of the matrix protein in favor of stem cell differentiation. Modification of the collagen by glycation might be used as a tool to improve natural polymers for material-induced stem cell differentiation in the future.

scholarly journals Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

2020 ◽  
Author(s):  
Nishir Mehta ◽  
Shahensha Shaik ◽  
Alisha Prasad ◽  
Ardalan Chaichi ◽  
Sushant P. Sahu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Adipogenic Differentiation ◽  
High Sensitivity ◽  
Stem Cell Differentiation ◽  
Metabolic Imaging ◽  
Spectral Signature ◽  
Ionization Mass ◽  
Label Free

ABSTRACTStem cell-based therapies carry significant promise for treating human diseases. However, clinical translation of stem cell transplants for effective therapy requires precise non-destructive evaluation of the purity of stem cells with high sensitivity (< 0.001% of the number of cells). Here, we report a novel methodology using hyperspectral imaging (HSI) combined with spectral angle mapping (SAM)-based machine learning analysis to distinguish differentiating human adipose derived stem cells (hASCs) from control stem cells. The spectral signature of adipogenesis generated by the HSI method enabled identification of differentiated cells at single cell resolution. The label-free HSI method was compared with the standard methods such as Oil Red O staining, fluorescence microscopy, and qPCR that are routinely used to evaluate adipogenic differentiation of hASCs. Further, we performed Raman microscopy and multiphoton-based metabolic imaging to provide complimentary information for the functional imaging of the hASCs. Finally, the HSI method was validated using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging of the stem cells. The study presented here demonstrates that multimodal imaging methods enable label-free identification of stem cell differentiation with high spatial and chemical resolution. This could provide a powerful tool to assess the safety and efficacy of stem cell-based regenerative therapies.

scholarly journals Emerging Potential of Exosomes on Adipogenic Differentiation of Mesenchymal Stem Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Yuxuan Zhong ◽  
Xiang Li ◽  
Fanglin Wang ◽  
Shoushuai Wang ◽  
Xiaohong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Adipogenic Differentiation ◽  
Stem Cell Differentiation ◽  
Cartilage Tissue ◽  
Differentiation Potential ◽  
Engineering Research ◽  
Adipose Tissue Engineering

The mesenchymal stem cells have multidirectional differentiation potential and can differentiate into adipocytes, osteoblasts, cartilage tissue, muscle cells and so on. The adipogenic differentiation of mesenchymal stem cells is of great significance for the construction of tissue-engineered fat and the treatment of soft tissue defects. Exosomes are nanoscale vesicles secreted by cells and widely exist in body fluids. They are mainly involved in cell communication processes and transferring cargo contents to recipient cells. In addition, exosomes can also promote tissue and organ regeneration. Recent studies have shown that various exosomes can influence the adipogenic differentiation of stem cells. In this review, the effects of exosomes on stem cell differentiation, especially on adipogenic differentiation, will be discussed, and the mechanisms and conclusions will be drawn. The main purpose of studying the role of these exosomes is to understand more comprehensively the influencing factors existing in the process of stem cell differentiation into adipocytes and provide a new idea in adipose tissue engineering research.

scholarly journals Emilin-2 is a component of bone marrow extracellular matrix regulating mesenchymal stem cell differentiation and hematopoietic progenitors

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Francesco Da Ros ◽  
Luca Persano ◽  
Dario Bizzotto ◽  
Mariagrazia Michieli ◽  
Paola Braghetta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Adipogenic Differentiation ◽  
Stem Cell Differentiation ◽  
Dependent Manner ◽  
Hematopoietic Stem

Abstract Background Dissection of mechanisms involved in the regulation of bone marrow microenvironment through cell–cell and cell–matrix contacts is essential for the detailed understanding of processes underlying bone marrow activities both under physiological conditions and in hematologic malignancies. Here we describe Emilin-2 as an abundant extracellular matrix component of bone marrow stroma. Methods Immunodetection of Emilin-2 was performed in bone marrow sections of mice from 30 days to 6 months of age. Emilin-2 expression was monitored in vitro in primary and mesenchymal stem cell lines under undifferentiated and adipogenic conditions. Hematopoietic stem cells and progenitors in bone marrow of 3- to 10-month-old wild-type and Emilin-2 null mice were analyzed by flow cytometry. Results Emilin-2 is deposited in bone marrow extracellular matrix in an age-dependent manner, forming a meshwork that extends from compact bone boundaries to the central trabecular regions. Emilin-2 is expressed and secreted by both primary and immortalized bone marrow mesenchymal stem cells, exerting an inhibitory action in adipogenic differentiation. In vivo Emilin-2 deficiency impairs the frequency of hematopoietic stem/progenitor cells in bone marrow during aging. Conclusion Our data provide new insights in the contribution of bone marrow extracellular matrix microenvironment in the regulation of stem cell niches and hematopoietic progenitor differentiation.

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