scholarly journals Polyelectrolyte Multilayer Films Modification with Ag and rGO Influences Platelets Activation and Aggregate Formation under In Vitro Blood Flow

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 859 ◽  
Author(s):  
Gabriela Imbir ◽  
Aldona Mzyk ◽  
Klaudia Trembecka-Wójciga ◽  
Ewa Jasek-Gajda ◽  
Hanna Plutecka ◽  
...  
Keyword(s):  
Plasma Proteins ◽  
Multilayer Films ◽  
Chemical Parameters ◽  
Polyelectrolyte Multilayer ◽  
Physico Chemical ◽  
Substrate Properties ◽  
Promising Solution ◽  
Blood Plasma Proteins ◽  
Chemical Cross Linking

Surface functionalization of materials to improve their hemocompatibility is a challenging problem in the field of blood-contacting devices and implants. Polyelectrolyte multilayer films (PEMs), which can mimic functions and structure of an extracellular matrix (ECM), are a promising solution to the urgent need for functional blood-contacting coatings. The properties of PEMs can be easily tuned in order to provide a scaffold with desired physico-chemical parameters. In this study chitosan/chondroitin sulfate (Chi/CS) polyelectrolyte multilayers were deposited on medical polyurethane. Afterwards PEMs were modified by chemical cross-linking and nanoparticles introduction. Coatings with variable properties were tested for their hemocompatibility in the cone-plate tester under dynamic conditions. The obtained results enable the understanding of how substrate properties modulate PEMs interaction with blood plasma proteins and the morphotic elements.

scholarly journals Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes

2019 ◽  
Vol 47 (12) ◽  
pp. 6195-6207 ◽  
Author(s):  
Marius Socol ◽  
Renjie Wang ◽  
Daniel Jost ◽  
Pascal Carrivain ◽  
Cédric Vaillant ◽  
...  
Keyword(s):  
Structural Properties ◽  
Single Particle Tracking ◽  
Chemical Parameters ◽  
Chromosome Conformation ◽  
Formation Reaction ◽  
Rouse Model ◽  
Physico Chemical ◽  
Capture Techniques

Abstract DNA folding and dynamics along with major nuclear functions are determined by chromosome structural properties, which remain, thus far, elusive in vivo. Here, we combine polymer modeling and single particle tracking experiments to determine the physico-chemical parameters of chromatin in vitro and in living yeast. We find that the motion of reconstituted chromatin fibers can be recapitulated by the Rouse model using mechanical parameters of nucleosome arrays deduced from structural simulations. Conversely, we report that the Rouse model shows some inconsistencies to analyze the motion and structural properties inferred from yeast chromosomes determined with chromosome conformation capture techniques (specifically, Hi-C). We hence introduce the Rouse model with Transient Internal Contacts (RouseTIC), in which random association and dissociation occurs along the chromosome contour. The parametrization of this model by fitting motion and Hi-C data allows us to measure the kinetic parameters of the contact formation reaction. Chromosome contacts appear to be transient; associated to a lifetime of seconds and characterized by an attractive energy of –0.3 to –0.5 kBT. We suggest attributing this energy to the occurrence of histone tail-DNA contacts and notice that its amplitude sets chromosomes in ‘theta’ conditions, in which they are poised for compartmentalization and phase separation.

Internal Composition versus the Mechanical Properties of Polyelectrolyte Multilayer Films: The Influence of Chemical Cross-Linking†

Langmuir ◽  
2009 ◽  
Vol 25 (24) ◽  
pp. 13809-13819 ◽  
Author(s):  
Thomas Boudou ◽  
Thomas Crouzier ◽  
Rachel Auzély-Velty ◽  
Karine Glinel ◽  
Catherine Picart
Keyword(s):  
Mechanical Properties ◽  
Multilayer Films ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer ◽  
Chemical Cross Linking

Chiral Forms of 4-(2',4'-Difluorobiphenyl-4-yl)-2-methyl-4-oxobutanoic Acid (Flobufen) and Its Metabolite.Synthesis and Basic Biological Properties

1997 ◽  
Vol 62 (3) ◽  
pp. 498-509 ◽  
Author(s):  
Miroslav Kuchař ◽  
Marie Poppová ◽  
Antonín Jandera ◽  
Vladimíra Panajotovová ◽  
Hana Zůnová ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Biological Activities ◽  
Biological Properties ◽  
Optical Isomers ◽  
Clinical Testing ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Oxobutanoic Acid

4-(2',4'-Difluorobiphenyl-4-yl)-2-methyl-4-oxobutanoic acid (1, flobufen) is subjected to clinical testing in the treatment of rheumatoid arthritis. Owing to the occurrence of a centre of chirality, the compound exists in two enantiomers, and its major human metabolite, viz. 4-(2',4'-difluorobiphenyl-4-yl)-4-hydroxy-2-methylbutanoic acid isolated in the lactone form (2), possesses two chiral centres, making possible the existence of four stereoisomers. All of the optical isomers of the substances 1 and 2 were prepared. For flobufen (1), the racemate was separated into the stereoisomers by using the salts 3 with R-(+)- or S-(-)-1-phenylethylamine. The pairs of stereoisomers of 2, obtained by reduction of R-(+)-flobufen or the S-(-)-enantiomer, were separated by column chromatography. The physico-chemical parameters of the optical isomers were determined and some biological activities were evaluated in both in vitro and in vivo models.

scholarly journals Improvement of Stability and Cell Adhesion Properties of Polyelectrolyte Multilayer Films by Chemical Cross-Linking

2004 ◽  
Vol 5 (2) ◽  
pp. 284-294 ◽  
Author(s):  
Ludovic Richert ◽  
Fouzia Boulmedais ◽  
Philippe Lavalle ◽  
Jérôme Mutterer ◽  
Emmanuelle Ferreux ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Multilayer Films ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer ◽  
Adhesion Properties ◽  
Chemical Cross Linking

scholarly journals A REVIEW ON PHYSICO-CHEMICAL PARAMETERS OF LIPOSOMAL DOXORUBICIN

2019 ◽  
pp. 1-5
Author(s):  
DEEPANMOL SINGH ◽  
SHILPA PAHWA
Keyword(s):  
Liposomal Doxorubicin ◽  
Bioequivalence Study ◽  
Formulation Development ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Characteristic Features ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Multiple Conditions

The development of a generic liposomal doxorubicin product requires the study of critical physicochemical properties of the formulation. Food and Drug Administration (FDA) draft guideline has suggested few parameters to be tested for in vitro bioequivalence study which include liposomal composition, state of encapsulated drug, internal environment, liposomal morphology and number of lamellae, lipid bilayer phase transition, liposomal size distribution, grafted Polyethylene Glycol (PEG) at liposomal surface, electric surface potential or charge and in vitro leakage under multiple conditions. Characteristic features of components of liposomal doxorubicin formulation and detail of parameters to be studied have been discussed. This review compile specific, current and historical research outcomes on in vitro analysis of liposomal doxorubicin and highlights the important features that have a critical impact on properties of liposomal doxorubicin formulation. It will provide a better insight to the generic manufacturers and will help them to identify the critical quality attributes during the formulation development phase.

scholarly journals The impact of the contact of venous human blood with sorbents on some of blood physicochemical parameters in vitro

2018 ◽  
Vol 17 (1) ◽  
pp. 50-60 ◽  
Author(s):  
S. I. Kuznetsov ◽  
O. P. Kirichuk ◽  
N. V. Burkova ◽  
A. A. Topko ◽  
V. A. Davankov ◽  
...  
Keyword(s):  
Venous Blood ◽  
Silica Gels ◽  
Coarse Grained ◽  
Chemical Parameters ◽  
Rotational Mode ◽  
Physico Chemical ◽  
Crosslinked Polystyrene ◽  
Acid Base Status ◽  
The Impact

Purpose of the study. To assess the effect of contact between hyper-crosslinked polystyrene (HCP) MN-202 and two silica gels (coarse-grained silica gel large-pore (CSL-2) and Silochrom S-120) with human venous blood in vitro conditions to change its physico-chemical parameters. Material and methods. In bench conditions the contact interaction of heparinized blood and sorbents in the rotational mode is carried out for 120 minutes Oxygen status, acid-base status (ABS), electrolytes and some metabolites were evaluated. The analyzer ABL 800 FLEX / 835 Version 6.13 Build 372 was used. Conclusion. HCP MN-202 and Silochrom s-120 can be used as neutral matrices to further modify and design specific hemocontact preparations.

scholarly journals Protective action of carnosine and organic lithium salts in case of ethanol-induced oxidative damage of proteins and lipids of blood plasma in healthy persons and alcoholic patients

2019 ◽  
Vol 65 (1) ◽  
pp. 28-32 ◽  
Author(s):  
V.D. Prokopieva ◽  
E.V. Plotnikov ◽  
E.G. Yarygina ◽  
N.A. Bokhan
Keyword(s):  
Blood Plasma ◽  
Plasma Proteins ◽  
Protective Action ◽  
Lithium Carbonate ◽  
Lithium Salts ◽  
Oxidized Proteins ◽  
Blood Plasma Proteins ◽  
Healthy Persons ◽  
In Blood Plasma

Organic lithium salts containing anionic components (succinate, fumarate, pyruvate and antioxidant ascorbate) were tested for protection of blood plasma proteins and lipids against ethanol-induced oxidation in vitro. We used normothymic lithium carbonate and well-known antioxidant dipeptide carnosine (b-alanyl-L-histidine) as the reference drugs. The oxidized proteins and lipids were determined by the level of carbonylated proteins (CP) and TBA-reactive products (TBA-RP), respectively. In alcoholic patients the level of oxidized proteins and lipids was higher than in healthy persons. Incubation of blood with ethanol resulted in an increase in oxidized proteins and lipids in blood plasma of healthy persons but had no influence on the level of CP and TBA-RP in blood plasma of alcoholic patients. Lithium carbonate, lithium ascorbate, and lithium succinate exhibited protective action against ethanol-induced oxidation of biomolecules of blood plasma of healthy people. These effects were comparable with carnosine action. The studied compounds had no effect on the level of CP and TBA-RP of blood plasma of alcoholic patients.

scholarly journals Cartwright's method as a physico-chemical marker of antimonium crudum biological effect

2021 ◽  
Vol 17 (2) ◽  
pp. 14-14
Author(s):  
Hannah MG Mota ◽  
Ana Carla Aparicio ◽  
Larissa Helen Silva Oliveira ◽  
Renata Rossettini Palombo Pedro ◽  
Sandra AG Pinto ◽  
...  
Keyword(s):  
Biological Effects ◽  
Electric Signal ◽  
Chemical Marker ◽  
Chemical Parameters ◽  
Monocyte Migration ◽  
Physico Chemical ◽  
Methylene Violet ◽  
One Way Anova

Mice bearing Leishmania (L) amazonensis infection and treated with Antimonium crudum (AC) 30cH presented significant reduction of the monocyte migration to the site of infection with clinical improvement. In vitro, the treatment of infected macrophages with AC 30cH produced inhibition of the parasite-induced peaks of CCL2 (a chemokine for monocytes migration) and inhibition of lysosome activity, explaining the results obtained previously in vivo. In the following studies, physical-chemical parameters of the remedy and respective controls were evaluated, to search for a correlation with the former described biological effects. The study of polarity changes in different water-based dilutions of AC using Cartwright´s method, revealed dilution-dependent variations in the absorbance of three solvatochromic dyes ET 33, BDN and methylene Violet (MV), used as “probes” to evaluate the dipole features of the medicine. The electrical activity of the homeopathic preparations appears to be dilution-dependent and related to their biological effects. Further experiments were performed using samples of the supernatant of infected macrophages after 96 hours of incubation with AC in different dilutions. These samples were processed using the same procedures as used for the original medicines and were analyzed by MV method. All tested potencies presented a sharp increase of absorbance at 580 nm, in relation to all controls (supernatant from untreated cells and cells treated with succussed water), as performed by one-way ANOVA, being F = 176.208; p = 0.001 and ?2 = 0.988. This results strongly suggest that biological systems could amplify the electric signal and the following changes in the medium polarity.

scholarly journals Assessment of Physico-chemicaal Properties, Antifungal and Anti-sprouting Efficacy of Essential oils (Moringa oleifera and Sesame indicum Oil)

2021 ◽  
Vol 4 (3) ◽  
pp. 59-71
Author(s):  
Abel Y.K. ◽  
Olaleye O.O. ◽  
Ayanda I.S. ◽  
Olasope T.D.
Keyword(s):  
Moringa Oleifera ◽  
Seed Oil ◽  
Chemical Properties ◽  
Seed Oils ◽  
Chemical Parameters ◽  
Ph Values ◽  
Peroxide Values ◽  
Physico Chemical ◽  
Antifungal Potential

This study was aimed at evaluating the physico-chemical properties, antifungal and anti-sprouting efficacy of Moringa oleifera and Sesame indicum seed oil extracts. Physico-chemical parameters were determined and compared for both oils. There was significant (p < 0.05) difference observed between the refractive values (1.4570 and 1.4633), free fatty acid (FFA) (2.04 and 3.21 mg KOHg-1), acid values (6.08 and 6.43 mg KOHg-1), pH values (4.55 and 5.33), saponification values (210.75 and 215.57 mg of KOHg-1), peroxide values (5.40 and 5.80 meqO2/kg), specific gravity (0.8897 to 0.9161 g/cm3) and yield (8.25% and 32.02%) for cold press extracts of Sesame and Moringa seed oils respectively. In vitro antifungal efficacy of both oils (M. oleifera and S. indicum) against Aspergillus niger shows a range of 43.63% to 58.18% inhibition. No anti-sprouting effect was recorded across all concentrations tested for the two oils. However, the antifungal potential of both seed oil extracts (Moringa and Sesame) exhibited moderate inhibitory properties with the highest percentage inhibition at 48 hrs.

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