Investigating the Ruthenium Metalation of Proteins: X-ray Structure and Raman Microspectroscopy of the Complex between RNase A and AziRu

2013 ◽  
Vol 52 (19) ◽  
pp. 10714-10716 ◽  
Author(s):  
Alessandro Vergara ◽  
Irene Russo Krauss ◽  
Daniela Montesarchio ◽  
Luigi Paduano ◽  
Antonello Merlino
Keyword(s):  
Raman Microspectroscopy ◽  
Rnase A ◽  
X Ray

Mapping the protein-binding sites for iridium(iii)-based CO-releasing molecules

2016 ◽  
Vol 45 (30) ◽  
pp. 12206-12214 ◽  
Author(s):  
Marco Caterino ◽  
Ariel A. Petruk ◽  
Alessandro Vergara ◽  
Giarita Ferraro ◽  
Daniela Marasco ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Binding ◽  
Binding Sites ◽  
Raman Microspectroscopy ◽  
Rnase A ◽  
Protein Binding Sites ◽  
X Ray ◽  
X Ray Crystallography ◽  
Model Protein ◽  
Circular Dichroïsm

Mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography have been used to investigate the reaction of CO-releasing molecule Cs2IrCl5CO with the model protein RNase A.

scholarly journals Reaction with Proteins of a Five-Coordinate Platinum(II) Compound

2019 ◽  
Vol 20 (3) ◽  
pp. 520 ◽  
Author(s):  
Giarita Ferraro ◽  
Tiziano Marzo ◽  
Maria Cucciolito ◽  
Francesco Ruffo ◽  
Luigi Messori ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Platinum Complexes ◽  
Rnase A ◽  
Side Chain ◽  
Planar Geometry ◽  
Ionization Mass ◽  
X Ray ◽  
X Ray Crystallography ◽  
Egg White Lysozyme ◽  
Square Planar

Stable five-coordinate Pt(II) complexes have been highlighted as a promising and original platform for the development of new cytotoxic drugs. Their interaction with proteins has been scarcely studied. Here, the reactivity of the five-coordinate Pt(II) compound [Pt(I)(Me) (dmphen)(olefin)] (Me = methyl, dmphen = 2,9-dimethyl-1,10-phenanthroline, olefin = dimethylfumarate) with the model proteins hen egg white lysozyme (HEWL) and bovine pancreatic ribonuclease (RNase A) has been investigated by X-ray crystallography and electrospray ionization mass spectrometry. The X-ray structures of the adducts of RNase A and HEWL with [Pt(I)(Me)(dmphen)(olefin)] are not of very high quality, but overall data indicate that, upon reaction with RNase A, the compound coordinates the side chain of His105 upon releasing the iodide ligand, but retains the pentacoordination. On the contrary, upon reaction with HEWL, the trigonal bi-pyramidal Pt geometry is lost, the iodide and the olefin ligands are released, and the metal center coordinates the side chain of His15 probably adopting a nearly square-planar geometry. This work underlines the importance of the combined use of crystallographic and mass spectrometry techniques to characterize, in detail, the protein–metallodrug recognition process. Our findings also suggest that five-coordinate Pt(II) complexes can act either retaining their uncommon structure or functioning as prodrugs, i.e., releasing square-planar platinum complexes as bioactive species.

Investigation of TiO2 Thin Coatings for Medical Applications by X-Ray Diffraction and Raman Microspectroscopy

2013 ◽  
Vol 203-204 ◽  
pp. 165-168
Author(s):  
Jozef Jasinski ◽  
Bogusław Rajchel ◽  
Malgorzata Lubas ◽  
Jarosław Jasiński ◽  
Barbara Kucharska ◽  
...  
Keyword(s):  
Titanium Surface ◽  
Single Layer ◽  
Fluidized Bed Reactor ◽  
Raman Microspectroscopy ◽  
X Ray Diffraction ◽  
Thin Coatings ◽  
X Ray ◽  
Microstructure Parameters ◽  
Formation Method ◽  
Confocal Raman Microspectroscopy

The titanium and titanium alloys endoprostheses surface may be improved by forming TiOx single layer or TiOx – hydroxyapatite multi – layer on their surface. Biocompatibility of TiOx layers depends highly on their microstructure, parameters of formation method and properties of substrate. Paper presents microstructure of the TiOx layer formed on titanium surface in fluidized bed reactor. In order to evaluate thickness, microstructure and Ti:O concentration ratio X-ray diffraction, Confocal Raman microspectroscopy methods are realized. Methods enables to define every type of TiOx structure simultaneously including rutile which dominates in investigated layers.

EXPRESS: Systematic Raman Spectroscopic Study of the Isomorphy Between the Arsenate Minerals Roselite, Wendwilsonite, Zincroselite, Brandtite, and Rruffite.

2020 ◽  
pp. 000370282098425
Author(s):  
J. Theo Kloprogge
Keyword(s):  
Crystal Structure ◽  
Photoelectron Spectroscopy ◽  
Symmetry Reduction ◽  
Raman Microspectroscopy ◽  
Tetrahedral Symmetry ◽  
Crystal Water ◽  
X Ray ◽  
Bending Modes ◽  
Analytical Tools ◽  
Non Destructive

In nature a wide variety of minerals are known with the general formula X2M(TO4)2·2(H2O) and an important group is formed by minerals with T = As. Most of these occur as minor or trace minerals in environments such as hydrothermal alterations of primary sulfides and arsenides. X-ray Photoelectron Spectroscopy (XPS) and Raman microspectroscopy have been utilized to study the chemistry and crystal structure of the roselite subgroup minerals, Ca2M(AsO4)2·2H2O (with M = Co, Mg, Mn, Zn, and Cu). The arsenate AsO4 stretching region exhibited minor differences between the roselite subgroup minerals, which can be explained by the ionic radius of the cation substituting on the M position in the structure. Multiple AsO4 antisymmetric stretching vibrations were found, pointing to a tetrahedral symmetry reduction. Similar observations were made for the corresponding bending modes. Bands around 450 cm-1 were attributed to ν4 bending modes. Several bands in the 300–350cm-1 region attributed to ν2 bending modes also provide evidence of symmetry reduction of the AsO4 anion. Two broad bands for roselite were found around 3330 and 3120 cm-1 and were attributed to the OH stretching bands of crystal water. These bands are accompanied by two bands around 1700 and 1610 cm-1 attributed to the corresponding OH-bending modes. In conclusion, both XPS and Raman spectroscopy have been shown here to be valuable non-destructive analytical tools to characterize these secondary arsenate minerals. X-ray Photoelectron Spectroscopy and Raman microspectroscopy allow the chemistry and molecular structure of the roselite subgroup minerals to be studied in a non-destructive way. The minerals in the roselite subgroup are easily distinguished based on their chemical composition as determined by XPS. As expected for minerals with the same crystal structure, similarities exist in the Raman spectra, sufficient differences exist to be able to identify these minerals.

Characterization of Wallpaintings from the Caliphal Baths of Cordoba (Spain) by X-Ray Diffraction and Raman Microspectroscopy

2018 ◽  
Vol 52 (3) ◽  
pp. 411-422 ◽  
Author(s):  
Daniel Cosano ◽  
Dolores Esquivel ◽  
Azahara Pérez ◽  
César Jiménez-Sanchidrián ◽  
Carlos M. Costa ◽  
...  
Keyword(s):  
Raman Microspectroscopy ◽  
X Ray Diffraction ◽  
X Ray

Unusual mode of protein binding by a cytotoxic π-arene ruthenium(ii) piano-stool compound containing an O,S-chelating ligand

2016 ◽  
Vol 45 (31) ◽  
pp. 12283-12287 ◽  
Author(s):  
Jana Hildebrandt ◽  
Helmar Görls ◽  
Norman Häfner ◽  
Giarita Ferraro ◽  
Matthias Dürst ◽  
...  
Keyword(s):  
Protein Binding ◽  
Cytotoxic Activity ◽  
Rnase A ◽  
X Ray ◽  
Chelating Ligand

A new π-arene Ru(ii) piano-stool compound, showing significant cytotoxic activityin vitro, was synthesized. The X-ray structure of this compound and that of its complex with RNase A were determined.

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