Correlation of Antioxidant Capacities to Oxygen Radical Scavenging Enzyme Activities in Blackberry

2000 ◽  
Vol 48 (11) ◽  
pp. 5672-5676 ◽  
Author(s):  
Hongjun Jiao ◽  
Shiow Y. Wang
Keyword(s):  
Enzyme Activities ◽  
Radical Scavenging ◽  
Oxygen Radical ◽  
Antioxidant Capacities

scholarly journals Free-radical-scavenging and antioxidant capacities with special emphasis on enzyme activities and in vitro studies in Caralluma flava N. E. Br.

2017 ◽  
Vol 32 (1) ◽  
pp. 156-162 ◽  
Author(s):  
Kandhan Karthishwaran ◽  
Salem Obaid Saeed Obaid Al Shamisi ◽  
Shyam Sreedhara Kurup ◽  
Sabitha Sakkir ◽  
Abdul Jaleel Cheruth
Keyword(s):  
Free Radical ◽  
Enzyme Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vitro Studies ◽  
Antioxidant Capacities

scholarly journals Comparison of the Phytochemical Properties, Antioxidant Activity and Cytotoxic Effect on HepG2 Cells in Mongolian and Taiwanese Rhubarb Species

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1217
Author(s):  
Ganbolor Jargalsaikhan ◽  
Jin-Yi Wu ◽  
Yen-Chou Chen ◽  
Ling-Ling Yang ◽  
Ming-Shun Wu
Keyword(s):  
Liver Cancer ◽  
Bioactive Compounds ◽  
Cytotoxic Effect ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Parp Cleavage ◽  
Total Phenolic ◽  
Antioxidant Capacities ◽  
Phytochemical Contents

The Mongolian rhubarb—Rheum undulatum L. (RU)—and Rumex crispus L. (RC)—a Taiwanese local rhubarb belonging to the family of Polygonaceae—are principal therapeutic materials in integrative medicine due to their rich quantities of bioactive compounds; however, their phytochemical and antioxidant properties, and anti-cancer activity is poorly investigated. Furthermore, the phytochemical characteristics of both species may be affected by their different geographical distribution and climatic variance. The current study aimed to compare RU with RC extracts in different polarity solvents (n-hexane, ethyl acetate, acetone, ethanol, and water) for their phytochemical contents including the total phenolic content (TPC), total anthraquinone content (TAC), total flavonoid content (TFC), antioxidant and free radical scavenging capacities, and anticancer ability on the HepG2 cell. Except for the n-hexane extract, all of the RU extracts had considerably higher TPCs than RC extracts, ranging from 8.39 to 11.16 mg gallic acid equivalent (GAE) per gram of dry weight, and the TPCs of each extract were also significantly correlated with their antioxidant capacities by ABTS, DPPH, and FRAP assays (p < 0.05). Moreover, there was no remarkable association between the antioxidant capacities and either TACs or TFCs in both the RU and RC extracts. Besides, high-performance liquid chromatography (HPLC) analysis revealed that both the RU and RC extracts contained chrysophanol, emodin, and physcion, and those bioactive compounds were relatively higher in the n-hexane solvent extracts. Additionally, we observed different levels of dose-dependent cytotoxic effects in all the extracts by cell viability assay. Notably, the ethanol extract of RU had a compelling cytotoxic effect with the lowest half-maximum inhibition concentration (IC50-171.94 ± 6.56 µg/mL at 48 h) among the RU extracts than the ethanol extract of RC. Interestingly, the ethanol extract of RU but not RC significantly induced apoptosis in the human liver cancer cell line, HepG2, with a distinct pattern in caspase-3 activation, resulting in increased PARP cleavage and DNA damage. In summary, Mongolian Rhubarb, RU, showed more phytochemical contents, as well as a higher antioxidant capacity and apoptotic effect to HepG2 than RC; thus, it can be exploited for the proper source of natural antioxidants and liver cancer treatment in further investigation.

Investigation of Free Radical Scavenging Enzyme Activities and Lipid Peroxidation in Human Placental Tissues With Miscarriage

2006 ◽  
Vol 13 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Aydan Biri ◽  
Mustafa Kavutcu ◽  
Nuray Bozkurt ◽  
Erdinç Devrim ◽  
Nilhan Nurlu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Enzyme Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging

Wet and dry extraction of coconut oil: impact on lipid metabolic and antioxidant status in cholesterol coadministered rats

2009 ◽  
Vol 87 (8) ◽  
pp. 610-616 ◽  
Author(s):  
K. Govindan Nevin ◽  
Thankappan Rajamohan
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Antioxidant Status ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Lipid Peroxide ◽  
Coconut Oil ◽  
Biologically Active ◽  
Antioxidant Enzyme Activities ◽  
Dry Process

Because coconut oil extracted by wet process (virgin coconut oil, VCO) is gaining popularity among consumers, this study was conducted to evaluate VCO compared with coconut oil extracted by dry process (copra oil, CO) for their influence on lipid parameters, lipid peroxidation, and antioxidant status in rats coadministered with cholesterol. VCO, CO, and cholesterol were fed in a semi-synthetic diet to 24 male Sprague–Dawley rats for 45 days. After the experimental period, lipid and lipid peroxide levels and antioxidant enzyme activities were observed. Chemical composition and antioxidant properties of the polyphenolic fraction from VCO and CO were also analyzed. The results showed that lipid and lipid peroxide levels were lower in VCO-fed animals than in animals fed either CO or cholesterol alone. Antioxidant enzyme activities in VCO-fed animals were comparable with those in control animals. Although the fatty acid profiles of both oils were similar, a significantly higher level of unsaponifiable components was observed in VCO. Polyphenols from VCO also showed significant radical-scavenging activity compared with those from CO. This study clearly indicates the potential benefits of VCO over CO in maintaining lipid metabolism and antioxidant status. These effects may be attributed in part to the presence of biologically active minor unsaponifiable components.

scholarly journals Analysis of Total Antioxidant Capacities of Various Seaweeds Grown off the Korean Coast (P06-110-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Chai-Hyeon Lee ◽  
Ye-Na Park ◽  
Hyeung-Rak Kim ◽  
Sang Gil Lee ◽  
Bohkyung Kim
Keyword(s):  
Functional Food ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Antioxidant Capacities ◽  
Scavenging Capacity ◽  
Total Antioxidant ◽  
Radical Generation ◽  
Dpph Radical Scavenging

Abstract Objectives It has been a long time since seaweeds have been used for food ingredients in Asian countries. Recently, a body of research has revealed the health benefits of bioactive compounds in seaweeds, especially their antioxidant capacities. Although numerous seaweeds inhabit in the ocean, only a small percentage has been explored for functional food. Therefore, the purpose of this study was to analyze the antioxidant capacities of various seaweeds grown off the Korean coast to screen for the potential functional food sources. Methods Ten ethanol extracts of Korean seaweeds were provided by the National Marine Biodiversity Institute of Korea, which included Scytosiphon gracilis, Scytosiphon lomentaria, Sargassum muticum, Sargassum confusum, Petrospongium rugosum, Sargassum fusiforme, Petalonia fascia, Sargassum nigrifolium, Ishige foliacea, and Myelophycus simplex. Hundred mg/L of samples in 80% methanol was used to measure their ABTS, DPPH, and superoxide radical scavenging activities. Mg vitamin C equivalent antioxidant capacity (VCEAC)/100 mg was used to express the ABTS and DPPH radical scavenging capacities. For the superoxide radical scavenging capacity, inhibition rate of superoxide radical generation (%) was calculated. Results Among the ten seaweeds, S. nigrifolium and I. foliacea exhibited the most significant radical scavenging capacities. DPPH radical scavenging capacities of I. foliacea and S. nigrifolium were 122.4 mg VCEAC/100 mg and 95.8 mg VCEAC/100 mg, respectively. For ABTS radical scavenging capacity, I. foliacea exhibited 178.5 mg VCEAC/100 mg, followed by S. nigrifolium as 80.9 mg VCEAC/100 mg. I. foliacea inhibited about 68% of superoxide radical generation followed by S. nigrifolium (40.6%) while vitamin C as positive control inhibited about 28.9% of superoxide radical generation. The antioxidant capacities measured by the three assays were positively correlated with each other. Conclusions The current study explored total antioxidant capacities of various Korean seaweeds and found I. foliacea and S. nigrifolium as the most potential antioxidant-rich food resources. Further research would be warranted to investigate bioactive compounds from S. nigrifolium and I. foliacea. Funding Sources This work was supported by the Pukyong National University Research Fund in 2018.

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