Biosynthesis of14C-Phytoene from Tomato Cell Suspension Cultures (Lycopersicon esculentum) for Utilization in Prostate Cancer Cell Culture Studies

2006 ◽  
Vol 54 (3) ◽  
pp. 747-755 ◽  
Author(s):  
Jessica K. Campbell ◽  
Randy B. Rogers ◽  
Mary Ann Lila ◽  
John W. Erdman
Keyword(s):  
Prostate Cancer ◽  
Cell Culture ◽  
Lycopersicon Esculentum ◽  
Cell Suspension ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Culture Studies

scholarly journals The Effect of Lycopene on Cancer Cell Apoptosis by Caspase-9 Concentration Measurement in Indonesian Human Prostate Cancer Cell Culture

2020 ◽  
Vol 8 (B) ◽  
pp. 952-956
Author(s):  
Tjahjodjati Tjahjodjati ◽  
Suwandi Sugandi ◽  
Rainy Umbas ◽  
Mieke Satari
Keyword(s):  
Prostate Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Caspase 9 ◽  
Human Prostate Cancer Cell ◽  
Treatment Groups ◽  
Significant Difference

BACKGROUND: Lycopene is an antioxidant that mostly found in daily ingredients such as tomatoes. Several studies have shown the lycopene potential in preventing prostate cancer. Nevertheless, the clinical use of lycopene as adjunctive therapy for prostate cancer is still under debate. AIM: The objective of the study was to determine the effect of lycopene on human prostate cancer cell culture growth by measuring caspase-9 concentration as a marker of the intrinsic pathway of apoptosis in cells. METHODS: This study was conducted on Indonesian prostate cancer cell culture from a patient with Gleason score 6, divided into 5 subgroups: 2 control groups and 3 treatment groups that were given 1 μM, 2 μM, and 4 μM of lycopene. Measurement of caspase-9 level was performed using enhanced chemiluminescence at 24, 28, and 72 h after lycopene addition in treatment groups. A comparative analysis was performed by two-way ANOVA. RESULTS: The result showed that there was a significant difference of mean caspase-9 levels in the provision of various concentrations of lycopene and time of observation (p < 0.05). Increased of mean caspase-9 levels started at 2 μM dose of lycopene at 48 h and 4 μM at 24 h (p < 0.05) and continue to rise at 72 h, but caspase-9 was not detected at 1 μM dose in every observation. CONCLUSION: There was a significant difference of mean caspase-9 levels in the provision of various concentrations of lycopene and time of observation.

Indole Alkaloids from Cell Suspension Cultures of Stemmadenia tomentosa and Voacanga africana

1982 ◽  
Vol 37 (10) ◽  
pp. 857-860 ◽  
Author(s):  
Joachim Stöckigt ◽  
Karl-Heinz Pawelka ◽  
Ana Rother ◽  
Brigitte Deus
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Growth Condition ◽  
Indole Alkaloids ◽  
Normal Growth ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Normal Growth Condition ◽  
Different Types

Abstract Cell suspension cultures of Stemmadenia tomentosa synthesized under normal growth condition the eight major indole alkaloids: (-)-tabersonine, (-)-minovincinine, (+)-conoflorine (voaphyl-line), condylocarpine, (+)-tubotaiwine (dihydrocondylocarpine), (-)-norfluorocurarine (vin-canine), (-)-vinervine, and (-)-coronaridine. These alkaloids consist of the three different types, Aspidosperma, Strychnos and Iboga. In contrast, cultures o f Voacanga africana produced mainly one alkaloid group (Aspidosperma-type) represented by (-)-tabersonine, lochnericine and (-)-minovincinine. Therefore this cell culture seems to be qualified for investigation concerning the biosynthesis of Aspidosperma alkaloids.

Elicitor-induced Changes of Enzyme Activities Related to Isoflavone and Pterocarpan Accumulation in Chickpea (Cicer arietinum L.) Cell Suspension Cultures

1988 ◽  
Vol 43 (7-8) ◽  
pp. 536-544 ◽  
Author(s):  
Susanne Daniel ◽  
Walter Hinderer ◽  
Wolfgang Barz
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Enzyme Activities ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Biochanin A ◽  
Primary Metabolism ◽  
Cicer Arietinum L ◽  
L Cell

The extractable activities of thirteen enzymes of primary and secondary metabolism have been measured in chickpea (Cicer arietinum L.) cell suspension cultures after treatment with an elicitor from the fungus Ascochyta rabiei (Pass.) Lab. The cell culture, derived from the A. rabiei resistant cultivar ILC 3279, constitutively accumulated the isoflavones biochanin A and formononetin together with their 7-O-glucosides and the 7-O-glucoside-6″-malonates. After elicitor application the cells rapidly form the pterocarpan phytoalexins medicarpin and maackiain. Among the enzymes of primary metabolism only the glucose 6-phosphate dehydrogenase exhibited a significant increase in activity with a maximum four hours after application of the elicitor. In phenylpropane metabolism the activities of phenylalanine ammonia lyase and chalcone synthase were enhanced by the elicitor and exhibited highest levels after four hours. In contrast the chalcone isomerase activity was not influenced by the elicitor. A substantial enhancement occurred with the isoflavone 7-O-glucosyltransferase activity eight hours after elicitor application. The results suggest that in this cell culture the elicitor-induced biosynthesis of pterocarpan phytoalexins was accompanied with a rapid and transient increase of those enzyme activities which are located at branching points of related pathways, i.e. pentose phosphate cycle, general phenylpropane metabolism, flavonoid formation and isoflavone conjugation.

Elicitor Induction of Cytochrome P-450 Monooxygenases in Cell Suspension Cultures of Chickpea (Cicer arietinum L.) and Their Involvement in Pterocarpan Phytoalexin Biosynthesis

1991 ◽  
Vol 46 (1-2) ◽  
pp. 58-66 ◽  
Author(s):  
Werner Gunia ◽  
Walter Hinderer ◽  
Uta Wittkampf ◽  
Wolfgang Barz
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Resistant Cultivar ◽  
Susceptible Cultivar ◽  
A Cell ◽  
Cell Culture Line ◽  
Cytochrome P 450

Abstract A yeast glucan elicitor causes the accumulation of the pterocarpan phytoalexins medicarpin and maackiain in chickpea (Cicer arietinum) cell suspension cultures established from seeds. A cell culture line from a chickpea cultivar resistant against its main fungal pathogen Ascochyta rabiei accumulates large amounts (944 nm ol/g fr. wt.) whereas a cell culture line from a susceptible cultivar accumulates only low amounts (38 nm ol/g fr. wt.) of the phytoalexins. This is consistent with differential accumulation of pterocarpan phytoalexins in intact plants [1], The first reactions in the pterocarpan-specific branch of biosynthesis are hydroxylation of the isoflavone intermediate form ononetin in position 2′ or 3′, catalyzed by microsomal cytochrome P-450 monooxygenases. Upon elicitation form ononetin 2′-hydroxylase undergoes a strong transient induction in the cell suspension culture of the resistant cultivar, whereas in the cell culture from the susceptible cultivar it is only slightly induced. In both cell suspension cul­tures the induction of cinnamic acid 4-hydroxylase and of form ononetin 3′-hydroxylase does not show a clear correlation with phytoalexin accumulation. Experiments with different elici­tor concentrations confirm that formononetin 2′-hydroxylase is much more induced in cell cul­tures from the resistant cultivar than from the susceptible one. It is concluded that the massive difference in phytoalexin accumulation between cell suspension cultures from the resistant and susceptible cultivar is determined mainly by the differential induction of form ononetin 2′-hydroxylase activity.

NON-DIFFERENTIAL SENSITIVITY TO THE HERBICIDE METRIBUZIN IN TOMATO CELL SUSPENSION CULTURES

1978 ◽  
Vol 58 (3) ◽  
pp. 775-778 ◽  
Author(s):  
B. E. ELLIS
Keyword(s):  
Lycopersicon Esculentum ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Differential Sensitivity ◽  
Lycopersicon Esculentum Mill ◽  
Differential Ability

Non-photosynthetic cell suspension cultures were established of four tomato (Lycopersicon esculentum Mill) cultivars with different degrees of seedling sensitivity to metribuzin. Growth in these cultures was not affected by levels of herbicide which are lethal to photosynthetic seedlings, but was inhibited by a concentration of 150 ppm. Although these cultivars display a differential ability to tolerate applications of metribuzin as seedlings, the cultures displayed no differential tolerance to the herbicide in the medium.

Regulation of immunophenotype modulation of monocytes-macrophages from M1 into M2 by prostate cancer cell-culture supernatant via transcription factor STAT3

2018 ◽  
Vol 196 ◽  
pp. 140-148 ◽  
Author(s):  
R. Solís-Martínez ◽  
M. Cancino-Marentes ◽  
G. Hernández-Flores ◽  
P. Ortiz-Lazareno ◽  
G. Mandujano-Álvarez ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Culture ◽  
Transcription Factor ◽  
Cancer Cell ◽  
Culture Supernatant ◽  
Prostate Cancer Cell ◽  
Cell Culture Supernatant

Elicitation of ß-l,3-Glucanase and Chitinase Activities in Cell Suspension Cultures of Ascochyta rabiei Resistant and Susceptible Cultivars of Chickpea ( Cicer avietinum)

1990 ◽  
Vol 45 (3-4) ◽  
pp. 233-239 ◽  
Author(s):  
Ralph Vogelsang ◽  
Wolfgang Barz
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Fungal Pathogen ◽  
Defense Mechanisms ◽  
Culture Medium ◽  
Cell Suspension Cultures ◽  
Chitinase Activity ◽  
Suspension Cultures ◽  
Ascochyta Rabiei ◽  
Incompatible Interaction

Abstract β-1,3-Glucanase and chitinase activities have been analyzed in cell suspension cultures of an A scochyta rabiei resistant ILC 3279 and a susceptible ILC 1929 cultivar of chickpea (Cicer arietinum) following treatment with an elicitor derived from this fungal pathogen. A facile method to determine both hydrolase activities in the cell culture medium was established. Significantly higher constitutive and elicitor-induced levels of both hydrolase activities were found in the medium of the ILC 3279 cell culture. The release of these enzyme activities is not due to cell lysis, but rather the consequence of a secretion mechanism. The cells of the resistant line contained a 5 times higher level of chitinase activity in comparison to the ILC 1929 cell culture, whereas the latter cells possessed a 3 times higher β -1,3-glucanase activity. The results are interpreted that accumulation of extracellular hydrolase activities may play an important role among the various plant defense mechanisms previously determined for the incompatible interaction between the resistant cultivar and its fungal pathogen.

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