Wilt Resistance in Tomatoes, Pectic Enzyme Formation by Fusarium oxysporum f. lycopersici on Susceptible and Resistant Tomato Stems

1962 ◽  
Vol 10 (2) ◽  
pp. 145-150 ◽  
Author(s):  
D. C. Deese ◽  
M. A. Stahmann
Keyword(s):  
Fusarium Oxysporum ◽  
Wilt Resistance ◽  
Pectic Enzyme ◽  
Enzyme Formation

scholarly journals Natural Variation in Portuguese Common Bean Germplasm Reveals New Sources of Resistance Against Fusarium oxysporum f. sp. phaseoli and Resistance-Associated Candidate Genes

2020 ◽  
Vol 110 (3) ◽  
pp. 633-647 ◽  
Author(s):  
Susana T. Leitão ◽  
Marcos Malosetti ◽  
Qijan Song ◽  
Fred van Eeuwijk ◽  
Diego Rubiales ◽  
...  
Keyword(s):  
Common Bean ◽  
Fusarium Oxysporum ◽  
Candidate Genes ◽  
Fusarium Wilt ◽  
Functional Markers ◽  
Gene Pools ◽  
Primary Metabolism ◽  
Sources Of Resistance ◽  
Wilt Resistance ◽  
Fusarium Wilt Resistance

Common bean (Phaseolus vulgaris) is one of the most consumed legume crops in the world, and Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli, is one of the major diseases affecting its production. Portugal holds a very promising common bean germplasm with an admixed genetic background that may reveal novel genetic resistance combinations between the original Andean and Mesoamerican gene pools. To identify new sources of Fusarium wilt resistance and detect resistance-associated single-nucleotide polymorphisms (SNPs), we explored, for the first time, a diverse collection of the underused Portuguese common bean germplasm by using genome-wide association analyses. The collection was evaluated for Fusarium wilt resistance under growth chamber conditions, with the highly virulent F. oxysporum f. sp. phaseoli strain FOP-SP1 race 6. Fourteen of the 162 Portuguese accessions evaluated were highly resistant and 71 intermediate. The same collection was genotyped with DNA sequencing arrays, and SNP–resistance associations were tested via a mixed linear model accounting for the genetic relatedness between accessions. The results from the association mapping revealed nine SNPs associated with resistance on chromosomes Pv04, Pv05, Pv07, and Pv08, indicating that Fusarium wilt resistance is under oligogenic control. Putative candidate genes related to phytoalexin biosynthesis, hypersensitive response, and plant primary metabolism were identified. The results reported here highlight the importance of exploring underused germplasm for new sources of resistance and provide new genomic targets for the development of functional markers to support selection in future disease resistance breeding programs.

PECTIC ENZYME SYNTHESIS IN RELATION TO VIRULENCE IN FUSARIUM OXYSPORUM F. LYCOPERSICI (SACC.) SNYDER AND HANSEN

1962 ◽  
Vol 40 (4) ◽  
pp. 533-541 ◽  
Author(s):  
R. Paquin ◽  
L. J. Coulombe
Keyword(s):  
Fusarium Oxysporum ◽  
Virulent Strain ◽  
Pectin Methylesterase ◽  
Enzyme Synthesis ◽  
Pectic Enzyme ◽  
Pectic Enzymes ◽  
Cultural Conditions ◽  
Pectin Content

The pectic enzymes produced by Fusarium oxysporum f. lycopersici appear to be causally related to the virulence of the pathogen. The effects of various cultural conditions on the synthesis of pectin methylesterase by two strains of the pathogen were investigated. The virulent strain grew better and produced more pectic enzymes than the avirulent one, and this difference became even more pronounced in media with high pectin content and when the temperature of incubation was raised from 15° to 28 °C. The correlation between the virulence of the pathogen and the production of the pectic enzymes is discussed.

Discovery of a new source of resistance to Fusarium oxysporum, cause of Fusarium wilt in Allium fistulosum, located on chromosome 2 of Allium cepa Aggregatum group

Genome ◽  
2012 ◽  
Vol 55 (11) ◽  
pp. 797-807 ◽  
Author(s):  
Hoa Q. Vu ◽  
Magdi A. El-Sayed ◽  
Shin-Ichi Ito ◽  
Naoki Yamauchi ◽  
Masayoshi Shigyo
Keyword(s):  
Fusarium Oxysporum ◽  
Allium Cepa ◽  
Fusarium Wilt ◽  
Root Exudates ◽  
Allium Fistulosum ◽  
Addition Lines ◽  
Wilt Resistance ◽  
Monosomic Addition ◽  
Monosomic Addition Lines ◽  
Fusarium Wilt Resistance

This study was carried out to evaluate the antifungal effect of Allium cepa Aggregatum group (shallot) metabolites on Fusarium oxysporum and to determine the shallot chromosome(s) related to Fusarium wilt resistance using a complete set of eight Allium fistulosum – shallot monosomic addition lines. The antifungal effects of hexane, butanol, and water extraction fractions from bulbs of shallot on 35 isolates of F. oxysporum were examined using the disc diffusion method. Only hexane and butanol fractions showed high antifungal activity. Shallot showed no symptom of disease after inoculation with F. oxysporum f. sp. cepae. The phenolic content of the roots and the saponin content of root exudates of inoculated shallot increased to much higher levels than those of the control at 3 days after inoculation. Application of freeze-dried shallot root exudates to seeds of A. fistulosum soaked in a spore suspension of F. oxysporum resulted in protection of seedlings against infection. Among eight monosomic addition lines and A. fistulosum, FF+2A showed the highest resistance to Fusarium wilt. This monosomic addition line also showed a specific saponin band derived from shallot on the thin layer chromatography profile of saponins in the eight monosomic addition lines. The chromosome 2A of shallot might possess some of the genes related to Fusarium wilt resistance.

scholarly journals QTL mapping for Fusarium wilt resistance based on the whole-genome resequencing and their association with functional genes in Raphanus sativus

2021 ◽  
Author(s):  
Yinbo Ma ◽  
Sushil Satish Chhapekar ◽  
Lu Lu ◽  
Xiaona Yu ◽  
Seungho Kim ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Phenotypic Variation ◽  
Pcr Analysis ◽  
Potential Candidate ◽  
Sequencing Data ◽  
Wilt Resistance ◽  
Protein Levels ◽  
Trait Locus ◽  
Fusarium Wilt Resistance

Abstract Fusarium wilt is an important disease of radish, leading to severe decrease in yield and quality. In this study, we used 180 F2 populations derived from a cross between radish inbred lines ‘YR4’ and ‘YR18’ to construct linkage group for the detecting quantitative trait locus (QTLs) related to Fusarium oxysporum resistance. Four QTLs related to Fusarium oxysporum resistance were detected on two linkage groups. Of the two major loci for FW resistance, FoRsR7.1FOR59 was delimited to the 2.18-Mb physical interval on chromosome R07, with a high LOD value (5.17–12.84). Additionally, it explained 9.34%-27.97% of the phenotypic variation in three inoculation tests. The other major QTL, FoRsR9.3FOR59, was also detected in all inoculation tests, but it had a relatively low value (3.38–4.52) and explained 6.24%-8.82% of the phenotypic variation. On the basis of the re-sequencing data for the parental lines, we identified 5 R-related genes and 13 unknown genes with sequence variations at the gene and protein levels. A semi-quantitative RT-PCR analysis revealed Rs382940 (TIR-NBS type) and Rs382200 (RLK type) were expressed only in ‘YR4’ from 0 to 6 days after the inoculation. Moreover, Rs382950 (TIR-NBS-LRR type) was more highly expressed in ‘YR4’ from 3 and 6 days after the inoculation. Therefore, these three genes are likely important for the FW resistance of radish. We identified several markers based on the potential candidate genes. These gene set might be useful for breeding system to introduce the Fusarium wilt resistance loci from ‘YR4’ to improve increasing tolerance to Fusarium oxysporum.

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