Quantitative Targeted Absolute Proteomic Analysis of Transporters, Receptors and Junction Proteins for Validation of Human Cerebral Microvascular Endothelial Cell Line hCMEC/D3 as a Human Blood–Brain Barrier Model

2012 ◽  
Vol 10 (1) ◽  
pp. 289-296 ◽  
Author(s):  
Sumio Ohtsuki ◽  
Chiemi Ikeda ◽  
Yasuo Uchida ◽  
Yumi Sakamoto ◽  
Florence Miller ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Proteomic Analysis ◽  
Microvascular Endothelial Cell ◽  
Endothelial Cell Line ◽  
Blood Brain Barrier Model ◽  
Barrier Model ◽  
Junction Proteins

scholarly journals The human brain endothelial cell line hCMEC/D3 as a human blood-brain barrier model for drug transport studies

2008 ◽  
Vol 107 (5) ◽  
pp. 1358-1368 ◽  
Author(s):  
Birk Poller ◽  
Heike Gutmann ◽  
Stephan Krähenbühl ◽  
Babette Weksler ◽  
Ignacio Romero ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Human Brain ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Drug Transport ◽  
Brain Endothelial Cell ◽  
Transport Studies ◽  
Blood Brain Barrier Model ◽  
Barrier Model

Development of immortalized human cerebromicrovascular endothelial cell line as an in vitro model of the human blood–brain barrier

1997 ◽  
Vol 11 (13) ◽  
pp. 1187-1197 ◽  
Author(s):  
Arumugam Muruganandam ◽  
Leonie Moorhouse Herx ◽  
Robert Monette ◽  
Jon P. Durkin ◽  
Danica B. Stanimirovic
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Endothelial Cell Line ◽  
Blood Brain ◽  
Vitro Model

scholarly journals Purine receptors and Ca2+ signalling in the human blood–brain barrier endothelial cell line hCMEC/D3

2011 ◽  
Vol 8 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Willem Bintig ◽  
Daniela Begandt ◽  
Barbara Schlingmann ◽  
Linda Gerhard ◽  
Maria Pangalos ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Brain Barrier ◽  
Purine Receptors ◽  
Endothelial Cell Line ◽  
Blood Brain

Endothelin-1 production by a microvascular endothelial cell line treated with Plasmodium falciparum parasitized red blood cells

2002 ◽  
Vol 103 (s2002) ◽  
pp. 464S-466S ◽  
Author(s):  
Nicoletta BASILICO ◽  
Livianna SPECIALE ◽  
Silvia PARAPINI ◽  
Pasquale FERRANTE ◽  
Donatella TARAMELLI
Keyword(s):  
Plasmodium Falciparum ◽  
Endothelial Cell ◽  
Red Blood Cells ◽  
Cell Line ◽  
Blood Cells ◽  
Microvascular Endothelial Cell ◽  
Microvascular Endothelium ◽  
Endothelial Cell Line ◽  
Endothelin 1 ◽  
Microvascular Endothelial

In this study, we investigated the production of endothelin 1 (ET-1) by a human microvascular endothelial cell line, HMEC-1, co-cultured with Plasmodium falciparum-parasitized red blood cells (pRBCs). The results indicate that hypoxia increased the basal level of ET-1 production by HMEC-1 cells after 24 or 48h of treatment. However, the co-incubation of HMEC-1 cells with pRBCs, but not with uninfected RBCs, induced a dose-dependent decrease of both constitutive and hypoxia-induced ET-1 production. The inhibition was not due to a decrease in cell viability, as lactate dehydrogenase release remained constant. These results indicate that pRBCs are able to interfere with both the constitutive and stimulated ET-1 release from the microvascular endothelium, thus inducing local modifications of the vascular tone and of the inflammatory response. This could be of relevance in the pathogenesis of the most severe forms of P. falciparum infections, such as cerebral malaria or malaria during pregnancy.

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