Role of Endosomal Escape for Disulfide-Based Drug Delivery from Colloidal Mesoporous Silica Evaluated by Live-Cell Imaging

The endoplasmic reticulum (ER) Ca2+ sensor STIM1 forms oligomers and translocates to ER–plasma membrane (PM) junctions to activate store-operated Ca2+ entry (SOCE) after ER Ca2+ depletion. STIM1 also interacts with EB1 and dynamically tracks microtubule (MT) plus ends. Nevertheless, the role of STIM1–EB1 interaction in regulating SOCE remains unresolved. Using live-cell imaging combined with a synthetic construct approach, we found that EB1 binding constitutes a trapping mechanism restricting STIM1 targeting to ER–PM junctions. We further showed that STIM1 oligomers retain EB1 binding ability in ER Ca2+-depleted cells. By trapping STIM1 molecules at dynamic contacts between the ER and MT plus ends, EB1 binding delayed STIM1 translocation to ER–PM junctions during ER Ca2+ depletion and prevented excess SOCE and ER Ca2+ overload. Our study suggests that STIM1–EB1 interaction shapes the kinetics and amplitude of local SOCE in cellular regions with growing MTs and contributes to spatiotemporal regulation of Ca2+ signaling crucial for cellular functions and homeostasis.

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Lipid order and molecular assemblies in the plasma membrane of eukaryotic cells

Biochemical Society Transactions ◽

10.1042/bst0371056 ◽

2009 ◽

Vol 37 (5) ◽

pp. 1056-1060 ◽

Cited By ~ 9

Author(s):

Marek Cebecauer ◽

Dylan M. Owen ◽

Anna Markiewicz ◽

Anthony I. Magee

Keyword(s):

Plasma Membrane ◽

Physical Properties ◽

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Eukaryotic Cells ◽

Dynamic Nature ◽

Membrane Components ◽

Technological Improvements

Multimolecular assemblies on the plasma membrane exhibit dynamic nature and are often generated during the activation of eukaryotic cells. The role of lipids and their physical properties in helping to control the existence of these structures is discussed. Technological improvements for live cell imaging of membrane components are also reviewed.

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cGMP Signaling in the Cardiovascular System—The Role of Compartmentation and Its Live Cell Imaging

International Journal of Molecular Sciences ◽

10.3390/ijms19030801 ◽

2018 ◽

Vol 19 (3) ◽

pp. 801 ◽

Cited By ~ 7

Author(s):

Nadja Bork ◽

Viacheslav Nikolaev

Keyword(s):

Cardiovascular System ◽

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Cgmp Signaling

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Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging

Nanomaterials ◽

10.3390/nano8060434 ◽

2018 ◽

Vol 8 (6) ◽

pp. 434 ◽

Cited By ~ 12

Author(s):

Roghayeh Erami ◽

Karina Ovejero ◽

Soraia Meghdadi ◽

Marco Filice ◽

Mehdi Amirnasr ◽

...

Keyword(s):

Mesoporous Silica ◽

Live Cell Imaging ◽

Cell Imaging ◽

Zinc Ion ◽

Live Cell ◽

Selective Detection

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Polyhedral borane derivatives: Unique and versatile structural motifs

Pure and Applied Chemistry ◽

10.1351/pac-con-12-02-11 ◽

2012 ◽

Vol 84 (11) ◽

pp. 2279-2288 ◽

Cited By ~ 29

Author(s):

M. Frederick Hawthorne ◽

Alexei Pushechnikov

Keyword(s):

Drug Delivery ◽

Aromatic Compounds ◽

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Molecular Volume ◽

Structural Motifs ◽

Modular Assembly ◽

Drug Delivery Vehicles ◽

Delivery Vehicles

Compounds with polyhedral borane moieties have demonstrated numerous unique properties for a variety of applications, including nanoelectronics, drug delivery vehicles, and live cell imaging. Polyhedral boranes are good pharmacophore analogs of carbocycles because polyhedral boranes are inherently insensitive to many undesirable enzymatic metabolic transformations typical for a majority of aromatic compounds. The defined shape, low molecular volume, and high 3D symmetry of the surface are useful for the application of the polyhedral borane scaffolds as universal and convenient spacers for the modular assembly approach with a controllable predisposition of peripheral groups.

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