Proteomic Screen for Multiprotein Complexes in Synaptic Plasma Membrane from Rat Hippocampus by Blue Native Gel Electrophoresis and Tandem Mass Spectrometry

2009 ◽  
Vol 8 (7) ◽  
pp. 3475-3486 ◽  
Author(s):  
Xuanwen Li ◽  
Chunliang Xie ◽  
Qihui Jin ◽  
Mingjun Liu ◽  
Quanyuan He ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Plasma Membrane ◽  
Tandem Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Rat Hippocampus ◽  
Tandem Mass ◽  
Synaptic Plasma Membrane ◽  
Proteomic Screen ◽  
Native Gel ◽  
Blue Native

S1890 Characterisation of Serum Proteins in Biliary Tract Cancer, PSC and Iac Using 2-Dimensional Difference Gel Electrophoresis and Tandem Mass Spectrometry

2010 ◽  
Vol 138 (5) ◽  
pp. S-811
Author(s):  
Neomal S. Sandanayake ◽  
John Sinclair ◽  
Fausto Andreola ◽  
Michael H. Chapman ◽  
Stephane Camuzeaux ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Biliary Tract ◽  
Biliary Tract Cancer ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Tandem Mass ◽  
Tract Cancer ◽  
Difference Gel Electrophoresis

scholarly journals Glycine Receptor Complex Analysis Using Immunoprecipitation‐Blue Native Gel Electrophoresis‐Mass Spectrometry

PROTEOMICS ◽  
2020 ◽  
Vol 20 (3-4) ◽  
pp. 1900403
Author(s):  
Sophie J. F. Spek ◽  
Frank Koopmans ◽  
Iryna Paliukhovich ◽  
Sarah L. Ramsden ◽  
Kirsten Harvey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Complex Analysis ◽  
Glycine Receptor ◽  
Receptor Complex ◽  
Native Gel ◽  
Blue Native

scholarly journals Proteomic Analysis of Cerebrospinal Fluid Changes Related to Postmortem Interval

2006 ◽  
Vol 52 (10) ◽  
pp. 1906-1913 ◽  
Author(s):  
Erin J Finehout ◽  
Zsofia Franck ◽  
Norman Relkin ◽  
Kelvin H Lee
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Tandem Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Protein Production ◽  
Postmortem Interval ◽  
Cytoskeletal Proteins ◽  
Tandem Mass ◽  
Concentration Change

Abstract Background: The study of proteins with altered production in postmortem cerebrospinal fluid (CSF) compared with antemortem CSF may improve the understanding of biochemical changes that occur immediately after death. Methods: Two CSF samples (1 antemortem and 1 postmortem) were collected from 7 patients and analyzed by 2-dimensional gel electrophoresis. An analysis was also performed to identify proteins that showed a correlation between concentration change and postmortem interval. Tandem mass spectrometry was used to identify the proteins. Results: Fifty-four protein spots were identified that showed a consistent and significant change in concentration in the postmortem CSF of all 7 patients (>3.5-fold, P <0.01). The proteins in these spots derive from a variety of functional groups, including cytoskeletal proteins, enzymes involved in glycolysis, and proteins that prevent oxidative stress. Fourteen protein spots were found to have an increase in production that correlated with postmortem interval. Conclusions: Changes in protein production of postmortem vs antemortem CSF were studied. The proteins observed to change production in the postmortem CSF include several proteins previously observed as potential stroke biomarkers.

Effect of fish skin mucus on the soluble proteome of Vibrio salmonicida analysed by 2-D gel electrophoresis and tandem mass spectrometry

2007 ◽  
Vol 42 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Inger Lin Uttakleiv Ræder ◽  
Steinar M. Paulsen ◽  
Arne O. Smalås ◽  
Nils Peder Willassen
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Tandem Mass ◽  
Fish Skin ◽  
Skin Mucus ◽  
Vibrio Salmonicida

Analysis of purified gp96 preparations from rat and mouse livers using 2-D gel electrophoresis and tandem mass spectrometry

Biochimie ◽  
2006 ◽  
Vol 88 (9) ◽  
pp. 1165-1174 ◽  
Author(s):  
B. Fairburn ◽  
M. Muthana ◽  
K. Hopkinson ◽  
L.K. Slack ◽  
S. Mirza ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Tandem Mass
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