Heat shock protein 70 gene polymorphism in Japanese patients with multiple sclerosis

2001 ◽  
Vol 58 (2) ◽  
pp. 93-96 ◽  
Author(s):  
M. Niino ◽  
S. Kikuchi ◽  
T. Fukazawa ◽  
I. Yabe ◽  
H. Sasaki ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Heat Shock ◽  
Gene Polymorphism ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Japanese Patients

Heat shock protein 70-hom gene polymorphism and protein expression in multiple sclerosis

2016 ◽  
Vol 298 ◽  
pp. 189-193 ◽  
Author(s):  
C. Boiocchi ◽  
M.C. Monti ◽  
C. Osera ◽  
G. Mallucci ◽  
C. Pistono ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Heat Shock ◽  
Gene Polymorphism ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Heat Shock Protein 70

scholarly journals Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat

2019 ◽  
Vol 19 (2) ◽  
pp. 122-128
Author(s):  
Kusnadidi Subekti ◽  
Dedi Duryadi Solihin ◽  
Rudi Afnan ◽  
Asep Gunawan ◽  
Cece Sumanri
Keyword(s):  
Heat Shock ◽  
Gene Polymorphism ◽  
Heat Shock Protein ◽  
Heat Tolerance ◽  
Heat Shock Protein 70 ◽  
Sanger Sequencing ◽  
Polymorphism Analysis ◽  
Chi Square ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (χ2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.  (The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck) ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (χ2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.

Study of the heat shock protein 70-1 gene polymorphism and the risk of nephropathy in type II diabetic patients

2014 ◽  
Vol 27 (3) ◽  
pp. 582
Author(s):  
EmanM Abd El Gayed ◽  
NaglaaM Ghanayem ◽  
MaathirK El-Shafie ◽  
EmanA.F. Badr ◽  
Elsayed Elnour ◽  
...  
Keyword(s):  
Heat Shock ◽  
Gene Polymorphism ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Diabetic Patients ◽  
Type Ii

scholarly journals Heat Shock Protein 70: Roles in Multiple Sclerosis

2012 ◽  
Vol 18 (6) ◽  
pp. 1018-1028 ◽  
Author(s):  
María José Mansilla ◽  
Xavier Montalban ◽  
Carmen Espejo
Keyword(s):  
Multiple Sclerosis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70

The potential association of heat shock protein 70-2 + 1267 A/G gene polymorphism with nephropathy in type II diabetic patients

2014 ◽  
Vol 27 (3) ◽  
pp. 589 ◽  
Author(s):  
MonaSalah El-Din Mahmoud Habib ◽  
NaglaaMohammed Ghanayem ◽  
SaidSayed Ahmed Khamis ◽  
MaathirKamel El-Shafie ◽  
YasserAbd El-Sattar El-Ghobashi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Gene Polymorphism ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Diabetic Patients ◽  
Type Ii ◽  
Potential Association

scholarly journals Up-regulation of inducible heat shock protein-70 expression in multiple sclerosis patients

Autoimmunity ◽  
2013 ◽  
Vol 47 (2) ◽  
pp. 127-133 ◽  
Author(s):  
María José Mansilla ◽  
Manuel Comabella ◽  
Jordi Río ◽  
Joaquín Castilló ◽  
Mireia Castillo ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Multiple Sclerosis Patients

scholarly journals Serum heat shock protein 70 levels as a biomarker for inflammatory processes in multiple sclerosis

2018 ◽  
Vol 4 (2) ◽  
pp. 205521731876719 ◽  
Author(s):  
Patricia Lechner ◽  
Dorothea Buck ◽  
Lisa Sick ◽  
Bernhard Hemmer ◽  
Gabriele Multhoff
Keyword(s):  
Multiple Sclerosis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Neurological Diseases ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Potential Biomarker ◽  
Inflammatory Processes

Background Inflammatory and neurodegenerative processes are hallmarks of multiple sclerosis (MS). The synthesis of the major stress-inducible heat shock protein 70 (Hsp70) is induced by inflammation. Objective The purpose of this study is to determine whether Hsp70 in serum can serve as a potential biomarker to distinguish inflammatory and neurodegenerative processes in MS. Methods Serum was obtained from 94 patients: 26 clinically isolated syndrome (CIS), 40 relapsing–remitting MS (RRMS), 19 secondary progressive MS (SPMS), and nine primary progressive MS (PPMS). As controls, serum samples were collected from patients with non-inflammatory neurological diseases (NINDs, n = 41), other inflammatory neurological diseases (OINDs, n = 28) and healthy donors (HDs, n = 114). Serum levels of Hsp70 were quantified using the enzyme-linked immunosorbent assay detecting free and liposomal Hsp70 (lipHsp70 ELISA). Results Patients with MS displayed significantly higher Hsp70 serum levels than HDs ( p < 0.001) and significantly lower levels than OINDs ( p = 0.001). A subgroup analysis revealed that Hsp70 serum levels of CIS/RRMS patients are significantly higher than those of patients with progressive MS (SPMS/PPMS) ( p < 0.05). Conclusion Inflammation causes the release of Hsp70 into the blood. As CIS/RRMS are associated with higher Hsp70 serum levels than progressive MS, serum Hsp70 levels might provide a marker for inflammatory processes.

Sign in / Sign up

Export Citation Format

Share Document