Characterization of embryogenesis-related proteins in alfalfa (Medicago sativa)

1996 ◽  
Vol 96 (4) ◽  
pp. 585-592 ◽  
Author(s):  
Randal W. Giroux ◽  
K. Peter Pauls
Keyword(s):  
Medicago Sativa ◽  
Related Proteins

scholarly journals Identification and Functional Characterization of a Cold-Related Protein, BcHHP5, in Pak-Choi (Brassica rapa ssp. chinensis)

2018 ◽  
Vol 20 (1) ◽  
pp. 93
Author(s):  
Jin Wang ◽  
Feiyi Huang ◽  
Xiong You ◽  
Xilin Hou
Keyword(s):  
Brassica Rapa ◽  
Abiotic Stresses ◽  
Quantitative Polymerase Chain Reaction ◽  
Functional Characterization ◽  
Regulation Mechanism ◽  
Pak Choi ◽  
Negative Effect ◽  
Polymerase Chain ◽  
Related Proteins

In plants, heptahelical proteins (HHPs) have been shown to respond to a variety of abiotic stresses, including cold stress. Up to the present, the regulation mechanism of HHP5 under low temperature stress remains unclear. In this study, BcHHP5 was isolated from Pak-choi (Brassica rapa ssp. chinensis cv. Suzhouqing). Sequence analysis and phylogenetic analysis indicated that BcHHP5 in Pak-choi is similar to AtHHP5 in Arabidopsis thaliana. Structure analysis showed that the structure of the BcHHP5 protein is relatively stable and highly conservative. Subcellular localization indicated that BcHHP5 was localized on the cell membrane and nuclear membrane. Furthermore, real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed that BcHHP5 was induced to express by cold and other abiotic stresses. In Pak-choi, BcHHP5-silenced assay, inhibiting the action of endogenous BcHHP5, indicated that BcHHP5-silenced might have a negative effect on cold tolerance, which was further confirmed. All of these results indicate that BcHHP5 might play a role in abiotic response. This work can serve as a reference for the functional analysis of other cold-related proteins from Pak-choi in the future.

scholarly journals Identification and characterization of a novel cytoskeleton-associated pp60src substrate.

1991 ◽  
Vol 11 (10) ◽  
pp. 5113-5124 ◽  
Author(s):  
H Wu ◽  
A B Reynolds ◽  
S B Kanner ◽  
R R Vines ◽  
J T Parsons
Keyword(s):  
Cell Structure ◽  
Transformed Cells ◽  
Cdna Clones ◽  
Amino Terminal ◽  
Multidomain Structure ◽  
Striking Change ◽  
Carboxyl Terminal ◽  
Amino Terminal Domain ◽  
Related Proteins

Transformation of cells by the src oncogene results in elevated tyrosine phosphorylation of two related proteins, p80 and p85 (p80/85). Immunostaining with specific monoclonal antibodies revealed a striking change of subcellular localization of p80/85 in src-transformed cells. p80/85 colocalizes with F-actin in peripheral extensions of normal cells and rosettes (podosomes) of src-transformed cells. Sequence analysis of cDNA clones encoding p80/85 revealed an amino-terminal domain composed of six copies of a direct tandem repeat, each repeat containing 37 amino acids, a carboxyl-terminal SH3 domain, and an interdomain region composed of a highly charged acidic region and a region rich in proline, serine, and threonine. The multidomain structure of p80/85 and its colocalization with F-actin in normal and src-transformed cells suggest that these proteins may associate with components of the cytoskeleton and contribute to organization of cell structure.

Identification of reproduction-related proteins and characterization of proteasome alpha 3 and proteasome beta 6 cDNAs in testes of the giant tiger shrimp Penaeus monodon

2012 ◽  
Vol 355 (1) ◽  
pp. 143-152 ◽  
Author(s):  
Sirawut Klinbunga ◽  
Sasithorn Petkorn ◽  
Suthathip Kittisenachai ◽  
Narumon Phaonakrop ◽  
Sittiruk Roytrakul ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Tiger Shrimp ◽  
Related Proteins

scholarly journals Nutritional Characterization of Medicago sativa (Alfalfa) for Feeding to Ruminants

2021 ◽  
Author(s):  
F. Almeida López ◽  
P. Toalombo Vargas ◽  
S. Jiménez Yánez ◽  
M. Chávez Haro
Keyword(s):  
Medicago Sativa ◽  
Crude Protein ◽  
Central Zone ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Nitrogen Free Extract ◽  
Palabras Clave ◽  
Nutritional Characteristics ◽  
Region Data

This study aimed to analyze the nutritional characteristics of Medicago sativa (Alfalfa) hay for the feeding of ruminants in the central zone of the Sierra Region. The study was conducted in the Laboratory of the Faculty of Pecaurias Sciences of the Higher Polytechnic School of Chimborazo. Cutting was carried out for 60 days in different areas of the central Sierra region. Data were analyzed using descriptive statistics. Results of the bromatological analysis showed that the average total humidity was 11.12 ± 0.20% in (Rp-00542) while the minimum was 7.79 ± 0.05% in (Rp-00224). Values for dry matter ranged between 91.25 ± 0.03% (Rp336) and 88.53 ± 0.09% (Rp492); dry base ash ranged between 12.51 ± 0.08% (Rp500) and 9.16 ± 0.08% (Rp521); dry organic matter ranged between 90.84 ± 0.13% (Rp521) and 87.49 ± 0.08% (Rp521); and dry crude protein ranged between 22.44 ± 0.26% (Rp492) and 18.73 ± 0.08% (Rp336). The following values were also obtained: 1.94 ± 0.93% dry ethereal extract; 32.06 ± 2.11% dry nitrogen free extract; 46.96 ± 3.78% dry neutral detergent fiber; 40.67 ± 3.36% dry acid detergent fiber; and 8.23 ± 0.64% dry lignin detergent acid. Therefore, it can be determined that forage mixture provides all of the nutrients to the species of zootechnical interest. Keywords: Medicago sativa, alfalfa, sierra centro, rumiants. RESUMEN En el Laboratorio de la Facultad de Ciencias Pecaurias de la Escuela Superior Politécnica de Chimborazo, se realizó la Caracterización Nutricional de henos de Medicago sativa (Alfalfa) para la alimentación de Rumiantes en la zona centro de la Región Sierra; Mediante estadística descriptiva se obtuvo los siguientes resultados del análisis bromatológico a los 60 días de corte en las diferentes zonas de la región Sierra - Centro, se puede observar que el mayor promedio de la humedad total fue 11,12 ± 0,20% en (Rp-00542) mientras que el menos fue 7,79 ± 0,05% en (Rp-00224); para Materia Seca-Total los valores se obtuvieron entre  91,25 ± 0,03% (Rp336) y 88,53 ± 0,09% (Rp492); Cenizas base seca 12,51 ± 0,08% (Rp500) y 9,16 ± 0,08% (Rp521); el mayor valor para % Materia Orgánica Base seca fue de 90,84 ± 0,13% (Rp521) y el menor con 87,49 ± 0,08% (Rp521); para % Proteína cruda base seca la mejor respuesta fue 22,44 ± 0,26 (Rp492) % y el menor  18,73 ± 0,08 (Rp336), Fibra cruda base seca; 1,94 ± 0,93% Extracto etéreo base seca; 32,06 ± 2,11% Extracto Libre de Nitrógeno base seca; 46,96 ± 3,78% Fibra Detergente Neutro base seca; 40,67 ± 3,36% Fibra Detergente Acida base seca; 8,23 ± 0,64% Lignina Detergente Acida base seca. Por lo que se puede determinar que la mezcla forrajera aporta todos los nutrientes a todas las especies de interés zootécnico. Palabras clave: Medicago sativa, alfalfa, sierra centro, rumiantes.

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