Candida africana asa species recovered from female genital specimens is highly close toC. albicans. The present study was conducted to discriminateC. africanafrom presumptive vaginalC. albicansstrains by molecular assay and evaluate their hemolysin activity, biofilm formation, and cohemolytic effect (CAMP) with vaginal bacterial flora. A total of 110 stock vaginalC. albicansisolates were examined byHWP1gene amplification. Hemolysin activity and the ability of biofilm formation were evaluated by blood plate assay and visual detection methods, respectively.Staphylococcus aureus,Staphylococcus epidermidis, andStreptococcus agalactiaewere used to evaluate the CAMP-like effects in Sabouraud blood agar media. Based on the size of the amplicons (941 bp), all isolates were identified asC. albicans. All samples were able to produce beta-hemolysin. Moreover, 69 out of 110 of the isolates (62.7%) were biofilm-positive, 54 out of 110Candidaisolates (49%) demonstrated cohemolytic effects withS. agalactiae, and 48 out of 110 showed this effect withS. aureus(43.6%). All isolates were CAMP-negative withS. epidermidis. We detected all isolates asCandida albicansand almost half of the isolates were CAMP-positive withS. aureusandS. agalactiae, suggesting that these bacteria increase the pathogenicity ofCandidain vaginal candidiasis.