Anthracycline antibiotics non-covalently incorporated into the block copolymer micelles: in vivo evaluation of anti-cancer activity

Objective: An Eco-friendly process of Green Synthesis of Copper Nano-particles (CuNPs) is an important aspect in the field of Nanotechnology using alternative feedstock, energy minimization, the design of less toxic and inherently safer chemicals. Methods: In this study, Copper Nano-Particles were synthesized by using isolated flavonoids to induce the reduction of Cu2+ions to CuNPs and also act as a capping and stabilizing agent. The solutions of CuSO4 and flavonoid were used as stock solutions for the preparation of CuNPs. Aqueous flavonoid(Quercetin) solution was mixed with CuSO4 solution separately. The reaction mixtures were immediately placed in a hot plate magnetic stirrer at 2000 rpm, 50-60 °C temperature and observed the change in colour of the solution from colourless to coloured solution. The synthesized CuNPs were characterized by various spectral methods for structural analysis of Nano-particles and In vitro and In vivo evaluation for anti-cancer activity. Results: The results described that the Copper Nano-particles are crystalline and amorphous with an average size of 295.4 nm and highly stable and having good hydrogen peroxide scavenging effect, reducing power and total antioxidant activity with the IC50value of 59.24μg/ml, 24.35μg/ml and16.83μg/ml respectively. The in vitro (HepG2 and MCF-7 cell lines) anti-cancer activity showing good results with IC50 values of 57.56 µg/ml for HepG2 cell line and 56.41 µg/ml for MCF-7 cell line. The in vivo anti-cancer activity also showing good results.

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Preparation and In Vivo Evaluation of Dichloro(1,2-Diaminocyclohexane)platinum(II)-Loaded Core Cross-Linked Polymer Micelles

Chemotherapy Research and Practice ◽

10.1155/2012/905796 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Hardeep S. Oberoi ◽

Natalia V. Nukolova ◽

Yi Zhao ◽

Samuel M. Cohen ◽

Alexander V. Kabanov ◽

...

Keyword(s):

Drug Loading ◽

Xenograft Model ◽

Tumor Xenograft ◽

Ovarian Cancer Cells ◽

In Vivo Evaluation ◽

Block Copolymer Micelles ◽

Copolymer Micelles ◽

High Drug Loading

The therapeutic performance of oxaliplatin can be improved by incorporating the central cis-dichloro(1,2-diaminocyclohexane)platinum(II) (DACHPt) motif into the core cross-linked block copolymer micelles. We describe here the preparation, cellular uptake, and in vivo evaluation of core cross-linked micelles loaded with DACHPt. Stable drug-loaded micelles were prepared at high drug loading (~25 w/w%) and displayed a considerably increased in vitro cytotoxicity compared to free oxaliplatin against A2780 ovarian cancer cells. The DACHPt-loaded micelle formulation was well tolerated in mice and exhibited improved antitumor activity than oxaliplatin alone in an ovarian tumor xenograft model.

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β-lactam Functionalized Poly(isoprene-b-ethylene oxide) Amphiphilic Block Copolymer Micelles as a New Nanocarrier System for Curcumin

Current Nanoscience ◽

10.2174/157341310791171090 ◽

2010 ◽

Vol 6 (3) ◽

pp. 277-284 ◽

Cited By ~ 7

Author(s):

Konstantinos Gardikis ◽

Konstantinos Dimas ◽

Aristidis Georgopoulos ◽

Eleni Kaditi ◽

Stergios Pispas ◽

...

Keyword(s):

Block Copolymer ◽

Ethylene Oxide ◽

Amphiphilic Block Copolymer ◽

Block Copolymer Micelles ◽

Copolymer Micelles

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Donnan Equilibria in Polymeric Micellar Systems with Weak Polyelectrolyte Shells: The Lowering of the pH Value

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19971730 ◽

1997 ◽

Vol 62 (11) ◽

pp. 1730-1736 ◽

Cited By ~ 4

Author(s):

Petr Munk ◽

Zdeněk Tuzar ◽

Karel Procházka

Keyword(s):

Ionic Strength ◽

Block Copolymer ◽

Ph Value ◽

Electrolyte Solutions ◽

Hydrogen Ions ◽

Micellar Systems ◽

Weak Electrolytes ◽

Block Copolymer Micelles ◽

Copolymer Micelles ◽

Polyelectrolyte Solutions

When two electrolyte solutions are separated and only some of the ions can cross the boundary, the concentrations of these ions are different on both sides of the boundary. This is the well-known Donnan effect. When weak electrolytes are involved, the imbalance includes also hydrogen ions: there is a difference of pH across the boundary and the dissociation of nondiffusible weak electrolytes is suppressed. The effect is very pronounced when the concentration of the weak electrolyte is high and ionic strength is low. The significance of this phenomenon is discussed for polyelectrolyte solutions, and particularly for block copolymer micelles with weak polyelectrolyte shells. The effect is quite dramatic in the latter case.

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