scholarly journals Differences in carbon source usage by dental plaque in children with and without early childhood caries

2017 ◽  
Vol 9 (12) ◽  
pp. e6-e6 ◽  
Author(s):  
Yan Zhao ◽  
Wen-Jie Zhong ◽  
Zhe Xun ◽  
Qian Zhang ◽  
Ye-Qing Song ◽  
...  
Keyword(s):  
Early Childhood ◽  
Carbon Source ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Childhood Caries

scholarly journals Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1645 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Boy M. Bachtiar
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Positive Correlation ◽  
Unstimulated Saliva ◽  
Student’S T ◽  
Total Bacteria ◽  
Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

Can insoluble polysaccharide concentration in dental plaque, sugar exposure and cariogenic microorganisms predict early childhood caries? A follow-up study

2015 ◽  
Vol 60 (8) ◽  
pp. 1091-1097 ◽  
Author(s):  
T.M. Parisotto ◽  
R. Stipp ◽  
L.K.A. Rodrigues ◽  
R.O. Mattos-Graner ◽  
L.S. Costa ◽  
...  
Keyword(s):  
Early Childhood ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Follow Up Study ◽  
Childhood Caries

scholarly journals Early Childhood Caries: Epidemiology, Aetiology, and Prevention

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
F. Meyer ◽  
J. Enax
Keyword(s):  
Early Childhood ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Oral Care ◽  
Rapid Progression ◽  
Poor Oral Hygiene ◽  
Caries Epidemiology ◽  
Oral Microorganisms ◽  
Childhood Caries

Early childhood caries (ECC) is one of the most prevalent diseases in children worldwide. ECC is driven by a dysbiotic state of oral microorganisms mainly caused by a sugar-rich diet. Additionally, poor oral hygiene or insufficient dental plaque removal leads to the rapid progression of ECC. ECC leads not only to dental destruction and pain with children, but also affects the quality of life of the caregivers. Children with extensive ECC are at high risk to develop caries with the permanent dentition or will have other problems with speaking and/or eating. To prevent ECC, several strategies should be taken into account. Children should brush their teeth with toothpastes containing gentle ingredients, such as mild surfactants and agents showing antiadherent properties regarding oral microorganisms. Parents/caregivers have to help their children with brushing the teeth. Furthermore, remineralizing and nontoxic agents should be included into the toothpaste formulation. Two promising biomimetic agents for children’s oral care are amorphous calcium phosphate [Cax(PO4)ynH2O] and hydroxyapatite [Ca5(PO4)3(OH)].

scholarly journals Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1645 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Boy M. Bachtiar
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Positive Correlation ◽  
Unstimulated Saliva ◽  
Student’S T ◽  
Total Bacteria ◽  
Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

Presence of mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries

2006 ◽  
Vol 51 (11) ◽  
pp. 1024-1028 ◽  
Author(s):  
Fabíola Galbiatti de Carvalho ◽  
Danilo Souza Silva ◽  
Josimeri Hebling ◽  
Luis Carlos Spolidorio ◽  
Denise Madalena Palomari Spolidorio
Keyword(s):  
Early Childhood ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Mutans Streptococci ◽  
Candida Spp ◽  
Carious Teeth ◽  
Childhood Caries

scholarly journals Relationship among microbiological composition and presence of dental plaque, sugar exposure, social factors and different stages of early childhood caries

2010 ◽  
Vol 55 (5) ◽  
pp. 365-373 ◽  
Author(s):  
Thaís Manzano Parisotto ◽  
Carolina Steiner-Oliveira ◽  
Cristiane Duque ◽  
Regina Célia Rocha Peres ◽  
Lidiany Karla Azevedo Rodrigues ◽  
...  
Keyword(s):  
Early Childhood ◽  
Social Factors ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Childhood Caries

scholarly journals S. mutans Serotype c, C. albicans, Oral Hygiene, and Decayed, Missing, and Filled Teeth in Early Childhood Caries

2020 ◽  
Vol 14 (1) ◽  
pp. 731-736
Author(s):  
Nurul Rahmah ◽  
Boy M. Bachtiar ◽  
Ferry P. Gultom ◽  
Retno D. Soejoedono ◽  
Endang W. Bachtiar
Keyword(s):  
Early Childhood ◽  
Oral Hygiene ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Molecular Mechanisms ◽  
New Technologies ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Childhood Caries

Purpose: The purpose of this study was to determine the quantity of Streptococcus mutans serotype c and Candida albicans antigens from dental plaque and their relationship with the Oral Hygiene Index Score (OHI-S) and Decayed, Missing, and Filled Teeth (DMFT) in Early Childhood Caries (ECC) and Severe Early Childhood Caries (S-ECC). Materials and Methods: This was a cross-sectional study, including 5 ECC and 32 S-ECC subjects. ECC was classified as a DMFT score < 4, and S-ECC was classified as a DMFT score ≥ 4 at 71 months of age. S. mutans serotype c and C. albicans antigens from dental plaque of ECC and S-ECC patients were measured using enzyme-linked immunosorbent assays. The optical density ​​of S. mutans serotype c and C. albicans antigens was determined at 450 nm. Correlations between S. mutans serotype c and C. albicans antigens and OHI-S in ECC and S-ECC were analyzed. Results: There were no significant differences in S. mutans serotype c and C. albicans antigens between ECC and S-ECC patients or between patients with moderate and good OHI-S scores. There was a negative correlation between S. mutans serotype c and C. albicans antigens in ECC patients (r = -0,62; p < 0.05). Conclusion: The increase in C. albicans suppresses S. mutans serotype c growth in ECC but not in S-ECC. The findings of this study could be used to discover the molecular mechanisms involved in bacterial-fungal relationships and the contribution of new technologies to reduce ECC.

scholarly journals Characterization of Supragingival Plaque and Oral Swab Microbiomes in Children With Severe Early Childhood Caries

2021 ◽  
Vol 12 ◽  
Author(s):  
Vivianne Cruz de Jesus ◽  
Mohd Wasif Khan ◽  
Betty-Anne Mittermuller ◽  
Kangmin Duan ◽  
Pingzhao Hu ◽  
...  
Keyword(s):  
Early Childhood ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Oral Microbiome ◽  
Rrna Genes ◽  
Oral Swab ◽  
Supragingival Plaque ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

The human oral cavity harbors one of the most diverse microbial communities with different oral microenvironments allowing the colonization of unique microbial species. This study aimed to determine which of two commonly used sampling sites (dental plaque vs. oral swab) would provide a better prediction model for caries-free vs. severe early childhood caries (S-ECC) using next generation sequencing and machine learning (ML). In this cross-sectional study, a total of 80 children (40 S-ECC and 40 caries-free) &lt; 72 months of age were recruited. Supragingival plaque and oral swab samples were used for the amplicon sequencing of the V4-16S rRNA and ITS1 rRNA genes. The results showed significant differences in alpha and beta diversity between dental plaque and oral swab bacterial and fungal microbiomes. Differential abundance analyses showed that, among others, the cariogenic species Streptococcus mutans was enriched in the dental plaque, compared to oral swabs, of children with S-ECC. The fungal species Candida dubliniensis and C. tropicalis were more abundant in the oral swab samples of children with S-ECC compared to caries-free controls. They were also among the top 20 most important features for the classification of S-ECC vs. caries-free in oral swabs and for the classification of dental plaque vs. oral swab in the S-ECC group. ML approaches revealed the possibility of classifying samples according to both caries status and sampling sites. The tested site of sample collection did not change the predictability of the disease. However, the species considered to be important for the classification of disease in each sampling site were slightly different. Being able to determine the origin of the samples could be very useful during the design of oral microbiome studies. This study provides important insights into the differences between the dental plaque and oral swab bacteriome and mycobiome of children with S-ECC and those caries-free.

scholarly journals Metagenomic Analysis Reveals Neisseria bacilliformis Variation in the Early Childhood Caries Plaque Microbiome

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Xiaofen Xiao ◽  
Shandan He ◽  
Fei He ◽  
Xiaoyun Wu ◽  
Yuyan Zheng
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Function Analysis ◽  
Acid Tolerance ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Metagenomic Assembly ◽  
Childhood Caries

The progression of early childhood caries (ECC) is caused by microbial colonized in dental plaque. However, the association framework both from 16s genus down to high resolution metagenomic strain level and from composition to genome function analysis on caries lacks. 16S rRNA sequence revealed the composition of 3–6 years dental caries (ECC, n = 29), and severe dental caries (SECC, n = 36) children are significantly different from caries-free controls (CF, n = 31). Especially, genus Neisseria is enriched in caries ( P < 0.05 ). Metagenomics sequence of 3 ECCs, 3 SECCs, and 3 CFs reveals Neisseria bacilliformis ATCC BAA-1200 in genus Neisseria is also significantly enriched in caries ( P < 0.05 ). Then, we recovered high-quality metagenomic assembly genomes (MAG), named bin 86, which have 99% identity with Neisseria bacilliformis ATCC BAA-1200 genome. Function analysis of Neisseria bacilliformis ATCC BAA-1200 genome shows its metabolism power of sugar and adhesion, colonization, acid production, and acid tolerance ability, which suggested Neisseria bacilliformis ATCC BAA-1200 may serve as a biomarker for childhood caries.

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