Urinary neutrophil gelatinase-associated lipocalin: potential biomarker for late-onset sepsis

Abstract Objective Pulmonary thromboembolism (PTE) is a clinical condition that can be lethal unless promptly diagnosed and treated. The objective was to evaluate the significance of serum neutrophil gelatinase-associated lipocalin (NGAL) in the diagnosis of PTE. Materials and methods In this study, 60 patients hospitalized for acute PTE between May 2015 and December 2016 were enrolled. PTE was diagnosed using spiral computed tomography angiography of the thorax. Cardiac enzyme levels, arterial blood gas, and echocardiography measurements were performed. Whole blood samples were drawn to measure serum NGAL before treatment. Results The PTE group comprised 34 women and 26 men, and the healthy control group included 22 women and 18 men. The mean ages of the patient and control groups were 70.3 ± 14.4 years and 69.0 ± 10.2 years, respectively. Serum NGAL was significantly higher in the patients than in the controls (88.6 ± 33.6 vs. 31.7 ± 10.0 ng/mL, p < 0.001, respectively). The optimal NGAL cut-off value was >50 ng/mL, the sensitivity was 100%, specificity was 98.3%, the negative predictive value was 100%, and the positive predictive value was 68%. Conclusion Serum NGAL is a new biomarker with high sensitivity and specificity to detect, diagnose, and exclude PTE.

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Urinary Neutrophil Gelatinase-Associated Lipocalin Is a Promising Biomarker for Late Onset Culture-Positive Sepsis in Very Low Birth Weight Infants

Pediatric Research ◽

10.1203/pdr.0b013e3181da75c1 ◽

2010 ◽

Vol 67 (6) ◽

pp. 636-640 ◽

Cited By ~ 29

Author(s):

Elvira Parravicini ◽

Sheri L Nemerofsky ◽

Kenneth A Michelson ◽

Trang K Huynh ◽

Meghan E Sise ◽

...

Keyword(s):

Birth Weight ◽

Low Birth Weight ◽

Very Low Birth Weight ◽

Late Onset ◽

Low Birth Weight Infants ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Neutrophil Gelatinase ◽

Culture Positive

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Urinary Neutrophil Gelatinase-Associated Lipocalin Is a Potential Biomarker for Renal Damage in Patients with Systemic Lupus Erythematosus

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/759313 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 15

Author(s):

Chun-Chen Yang ◽

Song-Chou Hsieh ◽

Ko-Jen Li ◽

Cheng-Han Wu ◽

Ming-Chi Lu ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Renal Damage ◽

Systemic Lupus ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Potential Biomarker ◽

Neutrophil Gelatinase

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Comparison of urine and serum neutrophil gelatinase-associated lipocalin after open and endovascular thoraco-abdominal aortic surgery and their meaning as indicators of acute kidney injury

VASA ◽

10.1024/0301-1526/a000736 ◽

2019 ◽

Vol 48 (1) ◽

pp. 79-87 ◽

Cited By ~ 4

Author(s):

Alexander Gombert ◽

Lukas Martin ◽

Ann Christina Foldenauer ◽

Clara Krajewski ◽

Andreas Greiner ◽

...

Keyword(s):

Acute Kidney Injury ◽

Serum Creatinine ◽

Kidney Injury ◽

Aortic Aneurysms ◽

Urine Ngal ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Abdominal Aortic ◽

Potential Biomarker ◽

Significant Difference ◽

Neutrophil Gelatinase

Abstract. Background: Neutrophil gelatinase-associated lipocalin (NGAL) has been described as a potential biomarker of acute kidney injury (AKI) in different settings, but its behaviour under influence of open and endovascular repair of thoraco-abdominal aortic aneurysms (TAAA) has not been assessed yet. In this study, the course of NGAL was observed and differences of serum- (sNGAL) and urine-NGAL (uNGAL) levels following TAAA repair, especially with regard to AKI, were evaluated. Patients and methods: In this retrospective single centre study, 52 patients (mean age 64.5 years, [43–85 years]), including 39 (75 %) men, were enrolled (2014–2015, 13.2 months mean follow-up). Levels of sNGAL and uNGAL were measured perioperatively for 48 hours on intensive care unit. Twenty-three patients were treated by endovascular and 29 by open TAAA-repair. Results: Logistic regression revealed an increase in NGAL (sNGAL p = 0.0263, uNGAL p = 0.0080) corresponding with an increase in serum creatinine within the first 48 hours. Fourteen patients (26.9 %) developed AKI and 11 (21.1 %) required dialysis. The course of NGAL differed significantly (uNGAL p < .0001, sNGAL p = 0.0002) between patients suffering from AKI requiring dialysis and patients without AKI. The predictive power of uNGAL was three times higher than that of sNGAL (estimate of the regression slope 0.1382 vs. 0.0460). No significant difference between patients undergoing open or endovascular TAAA repair regarding the perioperative course of sNGAL and uNGAL was observed. Conclusion: serum-NGAL and urine-NGAL correlate with serum creatinine levels and AKI requiring dialysis. Furthermore, the postoperative course of sNGAL and uNGAL after open and endovascular TAAA repair is not significantly different. Taken together, the results indicate that uNGAL and, to a lesser extent, sNGAL could be considered biomarkers for early detection of perioperative AKI after open and endovascular TAAA surgery.

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The Usefulness of Determining Neutrophil Gelatinase-Associated Lipocalin Concentration Excreted in the Urine in the Evaluation of Cyclosporine A Nephrotoxicity in Children with Nephrotic Syndrome

Disease Markers ◽

10.1155/2016/6872149 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Ewa Gacka ◽

Marcin Życzkowski ◽

Rafał Bogacki ◽

Andrzej Paradysz ◽

Lidia Hyla-Klekot

Keyword(s):

Nephrotic Syndrome ◽

Urine Concentration ◽

Control Group ◽

Renal Tubules ◽

Duration Of Treatment ◽

Serum Triglycerides ◽

Treatment Protocols ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Potential Biomarker ◽

Neutrophil Gelatinase

Introduction.The use of cyclosporine (CsA) in the treatment of nephrotic syndrome (NS) contributed to a significant reduction in the amount of corticosteroids used in therapy and its cumulative side effects. One of the major drawbacks of CsA therapy is its nephrotoxicity. Prolonged CsA treatment protocols require sensitive, easily available, and simple to measure biomarkers of nephrotoxicity. NGAL is an antibacterial peptide, excreted by cells of renal tubules in response to their toxic or inflammatory damage.Aim of the Study.The aim of this study was to assess the suitability of the NGAL concentration in the urine as a potential biomarker of the CsA nephrotoxicity.Material and Methods.The study was performed on a group of 31 children with NS treated with CsA. The control group consisted of 23 children diagnosed with monosyptomatic enuresis. The relationship between NGAL excreted in urine and the time of CsA treatment, concentration of CsA in blood serum, and other biochemical parameters was assessed.Results.The study showed a statistically significant positive correlation between urine NGAL concentration and serum triglycerides concentration and no correlation between C0 CsA concentration and other observed parameters of NS. The duration of treatment had a statistically significant influence on the NGAL to creatinine ratio.Conclusions.NGAL cannot be used alone as a simple CsA nephrotoxicity marker during NS therapy. Statistically significant correlation between NGAL urine concentration and the time of CsA therapy indicates potential benefits of using this biomarker in the monitoring of nephrotoxicity in case of prolonged CsA therapy.

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Proteomic Studies of Cholangiocarcinoma and Hepatocellular Carcinoma Cell Secretomes

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/437143 ◽

2010 ◽

Vol 2010 ◽

pp. 1-18 ◽

Cited By ~ 44

Author(s):

Chantragan Srisomsap ◽

Phannee Sawangareetrakul ◽

Pantipa Subhasitanont ◽

Daranee Chokchaichamnankit ◽

Khajeelak Chiablaem ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Lines ◽

Conditioned Media ◽

Lipocalin 2 ◽

Laminin 5 ◽

Normal Tissues ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Potential Biomarker ◽

Cholangiocarcinoma Cell ◽

Neutrophil Gelatinase

Cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) occur with relatively high incidence in Thailand. The secretome, proteins secreted from cancer cells, are potentially useful as biomarkers of the diseases. Proteomic analysis was performed on the secreted proteins of cholangiocarcinoma (HuCCA-1) and hepatocellular carcinoma (HCC-S102, HepG2, SK-Hep-1, and Alexander) cell lines. The secretomes of the five cancer cell lines were analyzed by SDS-PAGE combined with LC/MS/MS. Sixty-eight proteins were found to be expressed only in HuCCA-1. Examples include neutrophil gelatinase-associated lipocalin (lipocalin 2), laminin 5 beta 3, cathepsin D precursor, desmoplakin, annexin IV variant, and annexin A5. Immunoblotting was used to confirm the presence of lipocalin 2 in conditioned media and cell lysate of 5 cell lines. The results showed that lipocalin 2 was a secreted protein which is expressed only in the conditioned media of the cholangiocarcinoma cell line. Study of lipocalin 2 expression in different types of cancer and normal tissues from cholangiocarcinoma patients showed that lipocalin 2 was expressed only in the cancer tissues. We suggest that lipocalin 2 may be a potential biomarker for cholangiocarcinoma.

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