CPAP-induced airway hyper-reactivity in mice is modulated by hyaluronan synthase-3

2021 ◽  
Author(s):  
Catherine A. Mayer ◽  
Abhrajit Ganguly ◽  
Aubrey Mayer ◽  
Christina M. Pabelick ◽  
Y. S. Prakash ◽  
...  
Keyword(s):  
Hyaluronan Synthase

scholarly journals Self-regenerating giant hyaluronan polymer brushes

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Wenbin Wei ◽  
Jessica L. Faubel ◽  
Hemaa Selvakumar ◽  
Daniel T. Kovari ◽  
Joanna Tsao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polymer Brushes ◽  
Functional Materials ◽  
Polymer Physics ◽  
High Density ◽  
Hyaluronan Synthase ◽  
Dynamic Interface ◽  
Continuous Source

AbstractTailoring interfaces with polymer brushes is a commonly used strategy to create functional materials for numerous applications. Existing methods are limited in brush thickness, the ability to generate high-density brushes of biopolymers, and the potential for regeneration. Here we introduce a scheme to synthesize ultra-thick regenerating hyaluronan polymer brushes using hyaluronan synthase. The platform provides a dynamic interface with tunable brush heights that extend up to 20 microns – two orders of magnitude thicker than standard brushes. The brushes are easily sculpted into micropatterned landscapes by photo-deactivation of the enzyme. Further, they provide a continuous source of megadalton hyaluronan or they can be covalently-stabilized to the surface. Stabilized brushes exhibit superb resistance to biofilms, yet are locally digested by fibroblasts. This brush technology provides opportunities in a range of arenas including regenerating tailorable biointerfaces for implants, wound healing or lubrication as well as fundamental studies of the glycocalyx and polymer physics.

Expression of hyaluronan synthase-3 in porcine oviducal epithelium during oestrus

2003 ◽  
Vol 15 (2) ◽  
pp. 99 ◽  
Author(s):  
Paisan Tienthai ◽  
Naoko Kimura ◽  
Paraskevi Heldin ◽  
Eimei Sato ◽  
Heriberto Rodriguez-Martinez
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Hyaluronan Synthase ◽  
Critical Periods ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Mean Values ◽  
Polymerase Chain ◽  
Sperm Reservoir ◽  
Oviduct Fluid

Hyaluronan (HA) has been related to fertilization and embryo development in the pig. Furthermore, HA is present in pig oviduct fluid and the lining epithelium, particularly of the pre-ovulatory sperm reservoir. Because the mechanisms that regulate HA synthesis have not yet been clarified, semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was conducted to assess the expression of mRNAs of two HA-synthesizing enzymes (has2 and has3) in the oviduct epithelium (uterotubal junction, isthmus, ampullary–isthmic junction and ampulla segments) of non-inseminated (control) and inseminated (treatment) sows at pre-, peri- and post-ovulatory oestrus. Only has3 mRNA was detected; it was present in all tubal segments of both control and treatment samples. The level of has3 expression did not vary significantly between non-inseminated and inseminated specimens, but there was a tendency (NS) for increased mean values during the peri- and post-ovulatory stages compared with pre-ovulation. It is concluded that has3 is expressed by the porcine endosalpinx epithelium and the levels of expression do not vary during the critical periods of sperm transport and fertilization, despite fluctuating levels of HA in the tubal fluid at corresponding periods.

Endothelial hyaluronan synthase 3 aggravates acute colitis in an experimental model of inflammatory bowel disease

2021 ◽  
Author(s):  
Christian Hundhausen ◽  
Rebekka Schneckmann ◽  
Yanina Ostendorf ◽  
Jacqueline Rimpler ◽  
Anette von Glinski ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Experimental Model ◽  
Bowel Disease ◽  
Hyaluronan Synthase ◽  
Acute Colitis ◽  
Inflammatory Bowel

scholarly journals PROGNOSTIC VALUES OF MIDKINE, SYNDECAN-1, HYALURONAN SYNTHASE-2, SESTRIN-1, LAMININ SUBUNIT ALPHA-4 AND FIBULIN-3 FOR MALIGNANT PLEURAL MESOTHELIOMA

2021 ◽  
Author(s):  
HAKAN AKGUN ◽  
Selma Metintas ◽  
Guntulu Ak ◽  
Secil Demirkol ◽  
Murat Isbilen ◽  
...  
Keyword(s):  
Survival Time ◽  
Malignant Pleural Mesothelioma ◽  
Multivariate Analyses ◽  
Ex Vivo ◽  
Relevant Literature ◽  
Good Prognosis ◽  
Hyaluronan Synthase ◽  
Pleural Mesothelioma ◽  
Analysis Tool ◽  
Hyaluronan Synthase 2

IntroductionThe prognosis of malignant pleural mesothelioma (MPM) is poor with a limited survival time. In this study, we aimed to examine expression levels of genes selected from relevant literature and to utilize in silico methods to determine genes whose expression could reflect the prognosis of the patients with MPM by ex-vivo validation experimentsMaterial and methodsThe study group consisted of 54 MPM patients treated by chemotherapy. Expression of 6 genes; midkine (MDK), syndecan 1 (SDC1), hyaluronan synthase 2 (HAS2), sestrin 1 (SESN1), laminin subunit alpha 4 (LAMA4), and fibulin 3 (FBLN3) were examined by qPCR in tumor tissues. SESN1 and LAMA4 were identified using an in house R-based script “Unsupervised Survival Analysis Tool." MDK, SDC1, HAS2, and FBLN3 were selected from current literature. We used two housekeeping genes; glucose-6-phosphate dehydrogenase and TATA-box binding protein as controls.ResultsOf the patients, 43 (79.6%) had epithelioid mesothelioma. The median survival for all patients was 10 (±1.2 SE) months (CI 95%; 7.7-12.3). In multivariate analyses, MDK (p=0.007), HAS2 (p=0.008) and SESN1 (p=0.014) expression were related with survival time in whole group. In epithelioid type MPM patients, MDK (p=0.014), FBLN3 (p=0.029), HAS2 (p=0.014) and SESN1 (p=0.045) expression was related with survival time by multivariate analyses.ConclusionsHigh HAS2 and SESN1 expressions and low MDK are potential biomarkers of good prognosis in MPM. High HAS2 and SESN1 expression and low MDK and FBLN3 can also be utilized as biomarkers of good prognosis for epithelioid MPM. Those results should be further investigated in sera, plasma, and pleural effusions

Overexpression of Hyaluronan Synthase-2 Reduces the Tumorigenic Potential of Glioma Cells Lacking Hyaluronidase Activity

Neurosurgery ◽  
2002 ◽  
Vol 50 (6) ◽  
pp. 1311-1318 ◽  
Author(s):  
Bouchra Enegd ◽  
James A.J. King ◽  
Stan Stylli ◽  
Lucy Paradiso ◽  
Andrew H. Kaye ◽  
...  
Keyword(s):  
Glioma Cells ◽  
Hyaluronan Synthase ◽  
Hyaluronidase Activity ◽  
Tumorigenic Potential ◽  
Hyaluronan Synthase 2
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