scholarly journals Self-regenerating giant hyaluronan polymer brushes

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Wenbin Wei ◽  
Jessica L. Faubel ◽  
Hemaa Selvakumar ◽  
Daniel T. Kovari ◽  
Joanna Tsao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polymer Brushes ◽  
Functional Materials ◽  
Polymer Physics ◽  
High Density ◽  
Hyaluronan Synthase ◽  
Dynamic Interface ◽  
Continuous Source

AbstractTailoring interfaces with polymer brushes is a commonly used strategy to create functional materials for numerous applications. Existing methods are limited in brush thickness, the ability to generate high-density brushes of biopolymers, and the potential for regeneration. Here we introduce a scheme to synthesize ultra-thick regenerating hyaluronan polymer brushes using hyaluronan synthase. The platform provides a dynamic interface with tunable brush heights that extend up to 20 microns – two orders of magnitude thicker than standard brushes. The brushes are easily sculpted into micropatterned landscapes by photo-deactivation of the enzyme. Further, they provide a continuous source of megadalton hyaluronan or they can be covalently-stabilized to the surface. Stabilized brushes exhibit superb resistance to biofilms, yet are locally digested by fibroblasts. This brush technology provides opportunities in a range of arenas including regenerating tailorable biointerfaces for implants, wound healing or lubrication as well as fundamental studies of the glycocalyx and polymer physics.

Fabrication of Ordered Arrays of Gold Nanoparticles Coated with High-Density Polymer Brushes

2003 ◽  
Vol 42 (24) ◽  
pp. 2751-2754 ◽  
Author(s):  
Kohji Ohno ◽  
Kyoungmoo Koh ◽  
Yoshinobu Tsujii ◽  
Takeshi Fukuda
Keyword(s):  
Gold Nanoparticles ◽  
Polymer Brushes ◽  
High Density ◽  
Ordered Arrays

Precise Synthesis and Physicochemical Properties of High-Density Polymer Brushes designed with Poly(N-isopropylacrylamide)

2010 ◽  
Vol 43 (23) ◽  
pp. 9945-9956 ◽  
Author(s):  
Hiromasa Suzuki ◽  
Huda Muhammad Nurul ◽  
Takahiro Seki ◽  
Taisuke Kawamoto ◽  
Hisashi Haga ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Polymer Brushes ◽  
High Density

scholarly journals Effect of myeloperoxidase oxidation and N-homocysteinylation of high-density lipoprotein on endothelial repair function

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Takahiro Kameda ◽  
Yuna Horiuchi ◽  
Shitsuko Shimano ◽  
Kouji Yano ◽  
Shao-Jui Lai ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Wound Healing ◽  
High Density Lipoprotein ◽  
Troponin I ◽  
Density Lipoprotein ◽  
High Density ◽  
Major Protein ◽  
Oxidized Hdl ◽  
Healing Wound

Abstract Endothelial cell (EC) migration is essential for healing vascular injuries. Previous studies suggest that high-density lipoprotein (HDL) and apolipoprotein A-I (apoA-I), the major protein constituent of HDL, have endothelial healing functions. In cardiovascular disease, HDL is modified by myeloperoxidase (MPO) and N-homocysteine, resulting in apoA-I/apoA-II heterodimer and N-homocysteinylated (N-Hcy) apoA-I formation. This study investigated whether these modifications attenuate HDL-mediated endothelial healing. Wound healing assays were performed to analyze the effect of MPO-oxidized HDL and N-Hcy HDL in vitro. HDL obtained from patients with varying troponin I levels were also examined. MPO-oxidized HDL reduces EC migration compared to normal HDL in vitro, and N-Hcy HDL showed a decreasing trend toward EC migration. EC migration after treatment with HDL from patients was decreased compared to HDL isolated from healthy controls. Increased apoA-I/apoA-II heterodimer and N-Hcy apoA-I levels were also detected in HDL from patients. Wound healing cell migration was significantly negatively correlated with the ratio of apoA-I/apoA-II heterodimer to total apoA-II and N-Hcy apoA-I to total apoA-I. MPO-oxidized HDL containing apoA-I/apoA-II heterodimers had a weaker endothelial healing function than did normal HDL. These results indicate that MPO-oxidized HDL and N-Hcy HDL play a key role in the pathogenesis of cardiovascular disease.

Giant Hyaluronan Polymer Brushes Display Polyelectrolyte Brush Polymer Physics Behavior

2019 ◽  
Vol 8 (10) ◽  
pp. 1323-1327 ◽  
Author(s):  
Jessica L. Faubel ◽  
Riddhi P. Patel ◽  
Wenbin Wei ◽  
Jennifer E. Curtis ◽  
Blair K. Brettmann
Keyword(s):  
Polymer Brushes ◽  
Polymer Physics ◽  
Polyelectrolyte Brush ◽  
Brush Polymer

scholarly journals A Review on Plant Cellulose Nanofibre-Based Aerogels for Biomedical Applications

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1759 ◽  
Author(s):  
H.P.S. Abdul Khalil ◽  
A.S. Adnan ◽  
Esam Bashir Yahya ◽  
N.G. Olaiya ◽  
Safrida Safrida ◽  
...  
Keyword(s):  
Wound Healing ◽  
Biomedical Applications ◽  
Biomedical Application ◽  
Functional Materials ◽  
Plant Fibre ◽  
Potential Applications ◽  
Antimicrobial Film ◽  
Cellulose Nanomaterials ◽  
Dressing Materials ◽  
Antibiotic Delivery

Cellulose nanomaterials from plant fibre provide various potential applications (i.e., biomedical, automotive, packaging, etc.). The biomedical application of nanocellulose isolated from plant fibre, which is a carbohydrate-based source, is very viable in the 21st century. The essential characteristics of plant fibre-based nanocellulose, which include its molecular, tensile and mechanical properties, as well as its biodegradability potential, have been widely explored for functional materials in the preparation of aerogel. Plant cellulose nano fibre (CNF)-based aerogels are novel functional materials that have attracted remarkable interest. In recent years, CNF aerogel has been extensively used in the biomedical field due to its biocompatibility, renewability and biodegradability. The effective surface area of CNFs influences broad applications in biological and medical studies such as sustainable antibiotic delivery for wound healing, the preparation of scaffolds for tissue cultures, the development of drug delivery systems, biosensing and an antimicrobial film for wound healing. Many researchers have a growing interest in using CNF-based aerogels in the mentioned applications. The application of cellulose-based materials is widely reported in the literature. However, only a few studies discuss the potential of cellulose nanofibre aerogel in detail. The potential applications of CNF aerogel include composites, organic–inorganic hybrids, gels, foams, aerogels/xerogels, coatings and nano-paper, bioactive and wound dressing materials and bioconversion. The potential applications of CNF have rarely been a subject of extensive review. Thus, extensive studies to develop materials with cheaper and better properties, high prospects and effectiveness for many applications are the focus of the present work. The present review focuses on the evolution of aerogels via characterisation studies on the isolation of CNF-based aerogels. The study concludes with a description of the potential and challenges of developing sustainable materials for biomedical applications.

Sign in / Sign up

Export Citation Format

Share Document