scholarly journals Multiscale cytometry and regulation of 3D cell cultures on a chip

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Sébastien Sart ◽  
Raphaël F.-X. Tomasi ◽  
Gabriel Amselem ◽  
Charles N. Baroud
Keyword(s):  
Cell Cultures ◽  
3D Cell Cultures

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

scholarly journals Correction: Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 44397-44397
Author(s):  
Zhipan Wu ◽  
Rongfa Guan ◽  
Miao Tao ◽  
Fei Lyu ◽  
Guozhou Cao ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Cultures ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
3D Cell Cultures ◽  
2D And 3D

Correction for ‘Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures’ by Zhipan Wu, Rongfa Guan, Miao Tao et al., RSC Adv., 2017, 7, 12437–12445, DOI: 10.1039/C6RA27334C.

Loss of notochordal cell phenotype in 3D-cell cultures: implications for disc physiology and disc repair

2014 ◽  
Vol 134 (12) ◽  
pp. 1673-1681 ◽  
Author(s):  
G. W. Omlor ◽  
A. G. Nerlich ◽  
U. K. Tirlapur ◽  
J. P. Urban ◽  
T. Guehring
Keyword(s):  
Cell Cultures ◽  
Cell Phenotype ◽  
Notochordal Cell ◽  
3D Cell Cultures ◽  
Disc Repair

scholarly journals Generating linear oxygen gradients across 3D cell cultures with block-layered oxygen controlled chips (BLOCCs)

2020 ◽  
Vol 12 (1) ◽  
pp. 18-24 ◽  
Author(s):  
Matthew W. Boyce ◽  
William C. Simke ◽  
Rachael M. Kenney ◽  
Matthew R. Lockett
Keyword(s):  
Cell Cultures ◽  
Cell Microenvironment ◽  
3D Cell Cultures ◽  
Oxygen Gradients

BLOCCs are readily assembled structures of laser cut acrylic and silicone, capable of imposing physiologically relevant oxygen gradients across 3D cell cultures. With sensors and cell-based readouts, we quantified cell-microenvironment relationships.

scholarly journals Decellularized Human Gut as a Natural 3D Platform for Research in Intestinal Fibrosis

2019 ◽  
Vol 25 (11) ◽  
pp. 1740-1750 ◽  
Author(s):  
Paolo Giuffrida ◽  
Marco Curti ◽  
Walid Al-Akkad ◽  
Carin Biel ◽  
Claire Crowley ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Drug Testing ◽  
Biomarker Discovery ◽  
Disease Modeling ◽  
3D Culture ◽  
Protein Composition ◽  
Intestinal Fibrosis ◽  
3D Cell Cultures ◽  
3D Architecture ◽  
Inflammatory Bowel

Abstract Background The current methodologies for the identification of therapeutic targets for inflammatory bowel disease (IBD) are limited to conventional 2-dimensional (2D) cell cultures and animal models. The use of 3D decellularized human intestinal scaffolds obtained from surgically resected intestine and engineered with human intestinal cells may provide a major advancement in the development of innovative intestinal disease models. The aim of the present study was to design and validate a decellularization protocol for the production of acellular 3D extracellular matrix (ECM) scaffolds from the human duodenum. Methods Scaffolds were characterized by verifying the preservation of the ECM protein composition and 3D architecture of the native intestine and were employed for tissue engineering with primary human intestinal myofibroblasts for up to 14 days. Results Engrafted cells showed the ability to grow and remodel the surrounding ECM. mRNA expression of key genes involved in ECM turnover was significantly different when comparing primary human intestinal myofibroblasts cultured in 3D scaffolds with those cultured in standard 2D cultures on plastic dishes. Moreover, incubation with key profibrogenic growth factors such as TGFβ1 and PDGF-BB resulted in markedly different effects in standard 2D vs 3D cultures, further emphasizing the importance of using 3D cell cultures. Conclusions These results confirm the feasibility of 3D culture of human intestinal myofibroblasts in intestinal ECM scaffolds as an innovative platform for disease modeling, biomarker discovery, and drug testing in intestinal fibrosis.

scholarly journals High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures

2019 ◽  
Vol 11 (31) ◽  
pp. 28125-28137 ◽  
Author(s):  
Gabriele Tullii ◽  
Federica Giona ◽  
Francesco Lodola ◽  
Silvio Bonfadini ◽  
Caterina Bossio ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
Cell Cultures ◽  
High Aspect Ratio ◽  
Semiconducting Polymer ◽  
3D Cell Cultures

Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures

2020 ◽  
Vol 25 (3) ◽  
pp. 234-246
Author(s):  
Charles McRae White ◽  
Mark A. Haidekker ◽  
William S. Kisaalita
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Cell Biology ◽  
Low Cost ◽  
3D Cell Culture ◽  
Biomechanical Properties ◽  
Practical Applications ◽  
3D Cell Cultures

New insights into the biomechanical properties of cells are revealing the importance of these properties and how they relate to underlying molecular, architectural, and behavioral changes associated with cell state and disease processes. However, the current understanding of how these in vitro biomechanical properties are associated with in vivo processes has been developed based on the traditional monolayer (two-dimensional [2D]) cell culture, which traditionally has not translated well to the three-dimensional (3D) cell culture and in vivo function. Many gold standard methods and tools used to observe the biomechanical properties of 2D cell cultures cannot be used with 3D cell cultures. Fluorescent molecules can respond to external factors almost instantaneously and require relatively low-cost instrumentation. In this review, we provide the background on fluorescent molecular rotors, which are attractive tools due to the relationship of their emission quantum yield with environmental microviscosity. We make the case for their use in both 2D and 3D cell cultures and speculate on their fundamental and practical applications in cell biology.

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