scholarly journals Real-time multi-analyte online monitoring of 3d cell cultures by integrated enzyme-based biosensors in hanging drop networks

Author(s):  
P. M. Misun ◽  
J. Rothe ◽  
A. Hierlemann ◽  
O. Frey
Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.


RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 44397-44397
Author(s):  
Zhipan Wu ◽  
Rongfa Guan ◽  
Miao Tao ◽  
Fei Lyu ◽  
Guozhou Cao ◽  
...  

Correction for ‘Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures’ by Zhipan Wu, Rongfa Guan, Miao Tao et al., RSC Adv., 2017, 7, 12437–12445, DOI: 10.1039/C6RA27334C.


2014 ◽  
Vol 134 (12) ◽  
pp. 1673-1681 ◽  
Author(s):  
G. W. Omlor ◽  
A. G. Nerlich ◽  
U. K. Tirlapur ◽  
J. P. Urban ◽  
T. Guehring

2014 ◽  
Vol 87 ◽  
pp. 96-99 ◽  
Author(s):  
Oliver Frey ◽  
Patrick M. Misun ◽  
Jörg Rothe ◽  
Andreas Hierlemann

Sensor Review ◽  
2015 ◽  
Vol 35 (4) ◽  
pp. 348-356 ◽  
Author(s):  
Yongxing Guo ◽  
Dongsheng Zhang ◽  
Jianjun Fu ◽  
Shaobo Liu ◽  
Shengzhuo Zhang ◽  
...  

Purpose – The purpose of this paper is to investigate an online monitoring strategy that incorporates fiber Bragg gratings (FBGs) for deformation displacement detection, with the background that slope deformation monitoring is crucial to engineering safety supervision and disaster prevention. Design/methodology/approach – A “beam element” method has been proposed, introduced and experimentally verified in detail. The deformation displacement along a flexible bar can be obtained based on this method, using the distributed strain detected by the FBGs embedded in the bar. A novel sensor structure containing inclinometer casings and a series of connected flexible pipes with FBGs embedded has been proposed. Based on the features of this structure, two FBG deformation sensors have been manufactured and installed into a slope. A matched monitoring station which permits real-time supervision, warning and remote access across the Internet was established and operated. Findings – Displacement data from September 2013 to August 2014 are obtained, which is basically consistent with the practical situation. Originality/value – The FBG deformation sensors demonstrated a robust and reliable measurement performance, which is promising for real-time disaster warning in slope engineering.


2012 ◽  
Vol 134 (3) ◽  
Author(s):  
M. Torres ◽  
F. J. Muñoz ◽  
J. V. Muñoz ◽  
C. Rus

The Guidelines for the Assessment of Photovoltaic Plants provided by the Joint Research Centre (JRC) and the International Standard IEC 61724 recommend procedures for the analysis of monitored data to asses the overall performance of photovoltaic (PV) systems. However, the latter do not provide a well adapted method for the analysis of stand-alone photovoltaic systems (SAPV) with charge regulators without maximum power point tracker (MPPT). In this way, the IDEA Research Group has developed a new method that improves the analysis performance of these kinds of systems. Moreover, it has been validated an expression that compromises simplicity and accuracy when estimating the array potential in this kind of systems. SAPV system monitoring and performance analysis from monitored data are of great interest to engineers both for detecting a system malfunction and for optimizing the design of future SAPV system. In this way, this paper introduces an online monitoring system in real time for SAPV applications where the monitored data are processed in order to provide an analysis of system performance. The latter, together with the monitored data, are displayed on a graphical user interface using a virtual instrument (VI) developed in LABVIEW®. Furthermore, the collected and monitored data can be shown in a website where an external user can see the daily evolution of all monitored and derived parameters. At present, three different SAPV systems, installed in the Polytechnic School of University of Jaén, are being monitorized and the collected data are being published online in real time. Moreover, a performance analysis of these stand-alone photovoltaic systems considering both IEC 61724 and the IDEA Method is also offered. These three systems use the charge regulators more widespread in the market. Systems #1 and #2 use pulse width modulation (PWM) charge regulators, (a series and a shunt regulator, respectively), meanwhile System #3 has a charge regulator with MPPT. This website provides a tool that can be used not only for educational purposes in order to illustrate the operation of this kind of systems but it can also show the scientific and engineering community the main features of the system performance analysis methods mentioned above. Furthermore, it allows an external user to download the monitored and analysis data to make its own offline analysis. These files comply with the format proposed in the standard IEC 61724. The SAPV system monitoring website is now available for public viewing on the University of Jaén. (http://voltio.ujaen.es/sfa/index.html).


2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Sébastien Sart ◽  
Raphaël F.-X. Tomasi ◽  
Gabriel Amselem ◽  
Charles N. Baroud

2014 ◽  
Vol 6 (3) ◽  
pp. 366 ◽  
Author(s):  
Justin Melendez ◽  
Michael Patel ◽  
Benjamin L. Oakes ◽  
Ping Xu ◽  
Patrick Morton ◽  
...  

LWT ◽  
2016 ◽  
Vol 68 ◽  
pp. 532-540 ◽  
Author(s):  
Byeong-Sam Kim ◽  
Minwoo Lee ◽  
Ji-Young Kim ◽  
Jae-Yoon Jung ◽  
Junemo Koo

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