scholarly journals Complementary vibrational spectroscopy

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Kazuki Hashimoto ◽  
Venkata Ramaiah Badarla ◽  
Akira Kawai ◽  
Takuro Ideguchi
Keyword(s):  
Raman Scattering ◽  
Vibrational Spectroscopy ◽  
Infrared Absorption ◽  
Vibrational Spectra ◽  
Molecular Spectroscopy ◽  
High Spectral Resolution ◽  
Organic Liquids ◽  
Label Free ◽  
Molecular Fingerprint ◽  
Raman Scattering Spectroscopy

Abstract Vibrational spectroscopy, comprised of infrared absorption and Raman scattering spectroscopy, is widely used for label-free optical sensing and imaging in various scientific and industrial fields. The two molecular spectroscopy methods are sensitive to different types of vibrations and provide complementary vibrational spectra, but obtaining complete vibrational information with a single spectroscopic device is challenging due to the large wavelength discrepancy between the two methods. Here, we demonstrate simultaneous infrared absorption and Raman scattering spectroscopy that allows us to measure the complete broadband vibrational spectra in the molecular fingerprint region with a single instrument based on an ultrashort pulsed laser. The system is based on dual-modal Fourier-transform spectroscopy enabled by efficient use of nonlinear optical effects. Our proof-of-concept experiment demonstrates rapid, broadband and high spectral resolution measurements of complementary spectra of organic liquids for precise and accurate molecular analysis.

Raman and Infrared Studies of Substituted β-TCP

2011 ◽  
Vol 493-494 ◽  
pp. 225-230 ◽  
Author(s):  
Sophie Quillard ◽  
Charlotte Mellier ◽  
Renate Gildenhaar ◽  
Julien Hervelin ◽  
Philippe Deniard ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Solid Solutions ◽  
Infrared Absorption ◽  
Vibrational Spectra ◽  
Local Environment ◽  
Structural Investigations ◽  
Infrared Studies ◽  
Tricalcium Phosphates

Several substituted â-tricalcium phosphates have been prepared with different cations (monovalent, divalent and trivalent) and at various levels of substitution. Structural investigations have proved that fewer than ~10% wt substitution, the substituted compounds are isostructural to â- TCP, leading to solid solutions. These samples have been characterized by infrared and Raman spectroscopies. The vibrational spectra show mainly the bands related to the vibrations of PO43- tetrahedrons present in the structure. As Raman scattering and infrared absorption are local probes, the bands are sensitive to the local environment of the distinct tetrahedrons, related to the site of substitution and to the nature of the cations.

scholarly journals Multiplex coherent anti-Stokes Raman scattering highlights state of chromatin condensation in CH region

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tiffany Guerenne-Del Ben ◽  
Zakaniaina Rajaofara ◽  
Vincent Couderc ◽  
Vincent Sol ◽  
Hideaki Kano ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Raman Scattering ◽  
Chromatin Condensation ◽  
Cellular Level ◽  
High Spectral Resolution ◽  
Condensed State ◽  
Label Free ◽  
Coherent Raman ◽  
Wavenumber Region ◽  
Anti Stokes

Abstract Coherent Raman microscopy has become a powerful tool in label-free, non-destructive and fast cell imaging. Here we apply high spectral resolution multiplex coherent anti-Stokes Raman scattering (MCARS) microspectroscopy in the high wavenumber region to the study of the cell cycle. We show that heterochromatin - the condensed state of chromatin - can be visualised by means of the vibrational signature of proteins taking part in its condensation. Thus, we are able to identify chromosomes and their movement during mitosis, as well as structures like nucleoli and nuclear border in interphase. Furthermore, the specific organization of the endoplasmic reticulum during mitosis is highlighted. Finally, we stress that MCARS can reveal the biochemical impact of the fixative method at the cellular level. Beyond the study of the cell cycle, this work introduces a label-free imaging approach that enables the visualization of cellular processes where chromatin undergoes rearrangements.

scholarly journals Analysis of bodily fluids using vibrational spectroscopy: a direct comparison of Raman scattering and infrared absorption techniques for the case of glucose in blood serum

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3334-3346 ◽  
Author(s):  
Drishya Rajan Parachalil ◽  
Clément Bruno ◽  
Franck Bonnier ◽  
Hélène Blasco ◽  
Igor Chourpa ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Blood Serum ◽  
Vibrational Spectroscopy ◽  
Infrared Absorption ◽  
Blood Stream ◽  
Bodily Fluids ◽  
Patient Health ◽  
Crucial Information

Analysis of biomarkers present in the blood stream can potentially deliver crucial information on patient health and indicate the presence of numerous pathologies.

scholarly journals Photonic Time-Stretch Spectroscopy for Multiplex Stimulated Raman Scattering

2019 ◽  
Vol 205 ◽  
pp. 03003
Author(s):  
Francesco Saltarelli ◽  
Vikas Kumar ◽  
Daniele Viola ◽  
Francesco Crisafi ◽  
Fabrizio Preda ◽  
...  
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Materials Science ◽  
Stimulated Raman Scattering ◽  
High Sensitivity ◽  
Single Shot ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Novel Approach ◽  
Stimulated Raman

Stimulated Raman scattering spectroscopy enables label-free molecular identification, but its broadband implementation is technically challenging. We experimentally demonstrate a novel approach to multiplex stimulated Raman scattering based on photonic time stretch. A telecom fiber stretches the broadband femtosecond Stokes pulse after the sample to ∼15ns, mapping its spectrum in time. The signal is sampled through a fast oscilloscope, providing single-shot spectra at 80-kHz rate. We demonstrate high sensitivity in detecting the Raman vibrational modes of various samples over the entire high-frequency C-H stretching region. Our results pave the way to high-speed broadband vibrational imaging for materials science and biophotonics.

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