scholarly journals Elucidating the role of metal ions in carbonic anhydrase catalysis

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin Kyun Kim ◽  
Cheol Lee ◽  
Seon Woo Lim ◽  
Aniruddha Adhikari ◽  
Jacob T. Andring ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Metal Ions ◽  
Metal Ion ◽  
Catalytic Mechanism ◽  
Chemical Properties ◽  
Water Network ◽  
Native Metal ◽  
Trigonal Bipyramidal ◽  
Electrostatic Effect

Abstract Why metalloenzymes often show dramatic changes in their catalytic activity when subjected to chemically similar but non-native metal substitutions is a long-standing puzzle. Here, we report on the catalytic roles of metal ions in a model metalloenzyme system, human carbonic anhydrase II (CA II). Through a comparative study on the intermediate states of the zinc-bound native CA II and non-native metal-substituted CA IIs, we demonstrate that the characteristic metal ion coordination geometries (tetrahedral for Zn2+, tetrahedral to octahedral conversion for Co2+, octahedral for Ni2+, and trigonal bipyramidal for Cu2+) directly modulate the catalytic efficacy. In addition, we reveal that the metal ions have a long-range (~10 Å) electrostatic effect on restructuring water network in the active site. Our study provides evidence that the metal ions in metalloenzymes have a crucial impact on the catalytic mechanism beyond their primary chemical properties.

Why do zeolites induce an unprecedented electronic state on exchanged metal ions?

2017 ◽  
Vol 19 (36) ◽  
pp. 25105-25114 ◽  
Author(s):  
Akira Oda ◽  
Takahiro Ohkubo ◽  
Takashi Yumura ◽  
Hisayoshi Kobayashi ◽  
Yasushige Kuroda
Keyword(s):  
Metal Ions ◽  
Electronic State ◽  
Base Metal ◽  
Metal Ion ◽  
High Efficiency ◽  
Lewis Base ◽  
Mfi Zeolite ◽  
Adsorption Processes ◽  
Exact Position

Understanding the exact position and the detailed role of the Al array in zeolites is essential for elucidating the origin of unique properties and for designing zeolite materials with high efficiency in catalytic and adsorption processes. In this work, we advanced pivotal roles of Lewis base–metal ion bifunctionality caused by Al atoms arrayed circumferentially in the MFI-zeolite pores.

scholarly journals The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Yulia Sokurenko ◽  
Vera Ulyanova ◽  
Pavel Zelenikhin ◽  
Alexey Kolpakov ◽  
Dmitriy Blokhin ◽  
...  
Keyword(s):  
Metal Ions ◽  
Rna Structure ◽  
Metal Ion ◽  
Extracellular Enzymes ◽  
Bacillus Pumilus ◽  
Transition Metal Ions ◽  
The Body ◽  
Intestinal Microbiome ◽  
Phosphodiester Bond

Extracellular enzymes of intestinal microbiota are the key agents that affect functional activity of the body as they directly interact with epithelial and immune cells. Several species of theBacillusgenus, likeBacillus pumilus, a common producer of extracellular RNase binase, can populate the intestinal microbiome as a colonizing organism. Without involving metal ions as cofactors, binase depolymerizes RNA by cleaving the 3′,5′-phosphodiester bond and generates 2′,3′-cyclic guanosine phosphates in the first stage of a catalytic reaction. Maintained in the reaction mixture for more than one hour, such messengers can affect the human intestinal microflora and the human body. In the present study, we found that the rate of 2′,3′-cGMP was growing in the presence of transition metals that stabilized the RNA structure. At the same time, transition metal ions only marginally reduced the amount of 2′,3′-cGMP, blocking binase recognition sites of guanine at N7 of nucleophilic purine bases.

Role of histidine 64 in the catalytic mechanism of human carbonic anhydrase II studied with a site-specific mutant

Biochemistry ◽  
1989 ◽  
Vol 28 (19) ◽  
pp. 7913-7918 ◽  
Author(s):  
Chingkuang Tu ◽  
David N. Silverman ◽  
Cecilia Forsman ◽  
Bengt Harald Jonsson ◽  
Sven Lindskog
Keyword(s):  
Carbonic Anhydrase ◽  
Catalytic Mechanism ◽  
Carbonic Anhydrase Ii ◽  
Site Specific ◽  
Human Carbonic Anhydrase ◽  
A Site

scholarly journals On the unsuspected role of multivalent metal ions on the charge storage of a metal oxide electrode in mild aqueous electrolytes

2019 ◽  
Vol 10 (38) ◽  
pp. 8752-8763 ◽  
Author(s):  
Yee-Seul Kim ◽  
Kenneth D. Harris ◽  
Benoît Limoges ◽  
Véronique Balland
Keyword(s):  
Metal Oxide ◽  
Metal Ions ◽  
Metal Ion ◽  
Charge Storage ◽  
Aqueous Electrolytes ◽  
Electron Charge ◽  
Oxide Electrode ◽  
Metal Ion Complexes ◽  
Multivalent Metal

The hidden role of hexaaquo metal ion complexes in the proton-coupled electron charge storage at a metal oxide electrode.

Towards the role of metal ions in the structural variability of proteins: CdII speciation of a metal ion binding loop motif

Metallomics ◽  
2011 ◽  
Vol 3 (12) ◽  
pp. 1331 ◽  
Author(s):  
Attila Jancsó ◽  
Dániel Szunyogh ◽  
Flemming H. Larsen ◽  
Peter W. Thulstrup ◽  
Niels Johan Christensen ◽  
...  
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Structural Variability ◽  
Binding Loop

scholarly journals Olive Stone Delignification Toward Efficient Adsorption of Metal Ions

2021 ◽  
Vol 8 ◽  
Author(s):  
Ying Gao ◽  
Maria del Carmen Aliques Tomas ◽  
Jonas Garemark ◽  
Xia Sheng ◽  
Lars Berglund ◽  
...  
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Waste Product ◽  
Chemical Properties ◽  
Sodium Chlorite ◽  
Ion Adsorption ◽  
Metal Ion Adsorption ◽  
Optical Functions ◽  
Physical Chemical ◽  
Olive Stone

Olive stone is an important biomaterial waste product generated in large amount. As a lignocellulose material, olive stone could be a sustainable resource for biosorbents. In this work, olive stone powder delignification using sodium chlorite (NaClO2) was performed to enhance metal ion adsorption capacity. The influence of the treatment on olive stone powder physical-chemical properties was studied, including specific surface area, surface chemistry, morphology, etc. The white, delignified olive stone powder was applied for metal ions (Fe3+, Cu2+, and Zn2+) adsorption. Olive stone delignification not only increases the accessibility of the olive stone powder but also broadens the applications to materials design with optical functions by the generation of a white powder.

The role of metal ions in the virulence and viability of bacterial pathogens

2019 ◽  
Vol 47 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Stephanie L. Begg
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Molecular Mechanisms ◽  
Bacterial Pathogens ◽  
Ion Homeostasis ◽  
Transition Metal Ions ◽  
Metal Ion Homeostasis ◽  
And Function ◽  
Iron Manganese

AbstractMetal ions fulfil a plethora of essential roles within bacterial pathogens. In addition to acting as necessary cofactors for cellular proteins, making them indispensable for both protein structure and function, they also fulfil roles in signalling and regulation of virulence. Consequently, the maintenance of cellular metal ion homeostasis is crucial for bacterial viability and pathogenicity. It is therefore unsurprising that components of the immune response target and exploit both the essentiality of metal ions and their potential toxicity toward invading bacteria. This review provides a brief overview of the transition metal ions iron, manganese, copper and zinc during infection. These essential metal ions are discussed in the context of host modulation of bioavailability, bacterial acquisition and efflux, metal-regulated virulence factor expression and the molecular mechanisms that contribute to loss of viability and/or virulence during host-imposed metal stress.

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