scholarly journals Microsecond fingerprint stimulated Raman spectroscopic imaging by ultrafast tuning and spatial-spectral learning

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Haonan Lin ◽  
Hyeon Jeong Lee ◽  
Nathan Tague ◽  
Jean-Baptiste Lugagne ◽  
Cheng Zong ◽  
...  
Keyword(s):  
Cross Sections ◽  
High Speed ◽  
Large Scale ◽  
Stimulated Raman Scattering ◽  
Signal To Noise Ratio ◽  
Spectroscopic Imaging ◽  
Label Free ◽  
Tissue Samples ◽  
Fingerprint Region ◽  
Stimulated Raman

AbstractLabel-free vibrational imaging by stimulated Raman scattering (SRS) provides unprecedented insight into real-time chemical distributions. Specifically, SRS in the fingerprint region (400–1800 cm−1) can resolve multiple chemicals in a complex bio-environment. However, due to the intrinsic weak Raman cross-sections and the lack of ultrafast spectral acquisition schemes with high spectral fidelity, SRS in the fingerprint region is not viable for studying living cells or large-scale tissue samples. Here, we report a fingerprint spectroscopic SRS platform that acquires a distortion-free SRS spectrum at 10 cm−1 spectral resolution within 20 µs using a polygon scanner. Meanwhile, we significantly improve the signal-to-noise ratio by employing a spatial-spectral residual learning network, reaching a level comparable to that with 100 times integration. Collectively, our system enables high-speed vibrational spectroscopic imaging of multiple biomolecules in samples ranging from a single live microbe to a tissue slice.

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

scholarly journals Plasmon-enhanced stimulated Raman scattering microscopy with single-molecule detection sensitivity

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Cheng Zong ◽  
Ranjith Premasiri ◽  
Haonan Lin ◽  
Yimin Huang ◽  
Chi Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Detection Sensitivity ◽  
Single Molecule Detection ◽  
Label Free ◽  
Biomedical Systems ◽  
Stimulated Raman

AbstractStimulated Raman scattering (SRS) microscopy allows for high-speed label-free chemical imaging of biomedical systems. The imaging sensitivity of SRS microscopy is limited to ~10 mM for endogenous biomolecules. Electronic pre-resonant SRS allows detection of sub-micromolar chromophores. However, label-free SRS detection of single biomolecules having extremely small Raman cross-sections (~10−30 cm2 sr−1) remains unreachable. Here, we demonstrate plasmon-enhanced stimulated Raman scattering (PESRS) microscopy with single-molecule detection sensitivity. Incorporating pico-Joule laser excitation, background subtraction, and a denoising algorithm, we obtain robust single-pixel SRS spectra exhibiting single-molecule events, verified by using two isotopologues of adenine and further confirmed by digital blinking and bleaching in the temporal domain. To demonstrate the capability of PESRS for biological applications, we utilize PESRS to map adenine released from bacteria due to starvation stress. PESRS microscopy holds the promise for ultrasensitive detection and rapid mapping of molecular events in chemical and biomedical systems.

scholarly journals Photonic Time-Stretch Spectroscopy for Multiplex Stimulated Raman Scattering

2019 ◽  
Vol 205 ◽  
pp. 03003
Author(s):  
Francesco Saltarelli ◽  
Vikas Kumar ◽  
Daniele Viola ◽  
Francesco Crisafi ◽  
Fabrizio Preda ◽  
...  
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Materials Science ◽  
Stimulated Raman Scattering ◽  
High Sensitivity ◽  
Single Shot ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Novel Approach ◽  
Stimulated Raman

Stimulated Raman scattering spectroscopy enables label-free molecular identification, but its broadband implementation is technically challenging. We experimentally demonstrate a novel approach to multiplex stimulated Raman scattering based on photonic time stretch. A telecom fiber stretches the broadband femtosecond Stokes pulse after the sample to ∼15ns, mapping its spectrum in time. The signal is sampled through a fast oscilloscope, providing single-shot spectra at 80-kHz rate. We demonstrate high sensitivity in detecting the Raman vibrational modes of various samples over the entire high-frequency C-H stretching region. Our results pave the way to high-speed broadband vibrational imaging for materials science and biophotonics.

scholarly journals Label-Free Biomedical Imaging Using High-Speed Lock-In Pixel Sensor for Stimulated Raman Scattering

Sensors ◽  
2017 ◽  
Vol 17 (11) ◽  
pp. 2581 ◽  
Author(s):  
Kamel Mars ◽  
De Xing Lioe ◽  
Shoji Kawahito ◽  
Keita Yasutomi ◽  
Keiichiro Kagawa ◽  
...  
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Biomedical Imaging ◽  
Stimulated Raman Scattering ◽  
Label Free ◽  
Lock In ◽  
Stimulated Raman

scholarly journals Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells

2019 ◽  
Vol 63 (5) ◽  
pp. 2028-2034 ◽  
Author(s):  
Kristel Sepp ◽  
Martin Lee ◽  
Marie T. J. Bluntzer ◽  
G. Vignir Helgason ◽  
Alison N. Hulme ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Intracellular Distribution ◽  
Live Cells ◽  
Label Free ◽  
Stimulated Raman
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