scholarly journals Detection of exogenous sugars in pineapple juice using compound-specific stable hydrogen isotope analysis

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Simon D. Kelly ◽  
Aiman Abrahim ◽  
Peter Rinke ◽  
Andrew Cannavan
Keyword(s):  
Low Cost ◽  
Isotope Analysis ◽  
Isotope Ratio Mass Spectrometry ◽  
Cane Sugar ◽  
Isotopic Ratios ◽  
C4 Plant ◽  
Pineapple Juice ◽  
Exchangeable Hydrogen ◽  
Isotopic Methods ◽  
Stable Hydrogen Isotope

AbstractAn improved procedure for determining 2H/1H isotope ratios, using gas chromatography-isotope ratio mass spectrometry, has been used to detect the addition of exogenous C4-plant-derived sugars to pineapple juice. Isotopic techniques are commonly used to identify the addition of low-cost sugars to fruit juices and are difficult to subvert as it is not economically viable to change the isotopic ratios of the sugars. However, the addition of cane sugar to pineapple juice has presented a significant challenge that is only detected by site-specific 13C analysis of the methyl and methylene positions of ethanol derived from pineapple sugars, measured by nuclear magnetic resonance. This new GC-IRMS-based procedure utilises the trifluoroacetate derivative of sucrose to allow direct measurement of the carbon-bound non-exchangeable hydrogen. This provides advantages over alternative isotopic methods in terms of analysis time and sensitivity. This feasibility study has demonstrated the potential to reliably differentiate between authentic pineapple juices and those adulterated with commercial beet and cane sucrose.

scholarly journals Mass spectrometric measurement of hydrogen isotope fractionation for the reactions of chloromethane with OH and Cl

2018 ◽  
Author(s):  
Frank Keppler ◽  
Enno Bahlmann ◽  
Markus Greule ◽  
Heinz Friedrich Schöler ◽  
Julian Wittmer ◽  
...  
Keyword(s):  
Hydrogen Isotope ◽  
Isotope Fractionation ◽  
Isotope Effects ◽  
Isotope Analysis ◽  
Isotope Ratio Mass Spectrometry ◽  
Detailed Knowledge ◽  
Mass Spectrometric Measurement ◽  
Isotopic Analyses ◽  
Chlorine Radicals ◽  
Stable Hydrogen Isotope

Abstract. Chloromethane (CH3Cl) is an important provider of chlorine to the stratosphere but yet lacks detailed knowledge of its budget. Stable isotope analysis is potentially a powerful tool to constrain CH3Cl flux estimates. The largest degree of isotope fractionation is expected to occur for deuterium in CH3Cl in the hydrogen abstraction reactions with its main sink reactant tropospheric OH and its minor sink reactant Cl atoms. We determined the isotope fractionation by stable hydrogen isotope analysis of the fraction of CH3Cl remaining after reaction with hydroxyl and chlorine radicals in a 3.5 m3 Teflon smog-chamber at 293 ± 1 K. We measured the increasing stable hydrogen isotope values of the unreacted CH3Cl using compound specific thermal conversion isotope ratio mass spectrometry. The isotope fractionations of CH3Cl for the reactions with hydroxyl and chlorine radicals were found to be −242 ± 7 mUr (or ‰) and −280 ± 11 mUr, respectively. For comparison, we performed similar experiments using methane (CH4) as the target compound with OH and obtained a fractionation constant of −205 ± 6 mUr which is in good agreement with values previously reported. The observed large kinetic isotope effects are helpful when employing isotopic analyses of CH3Cl in the atmosphere to improve our knowledge of its atmospheric budget.

Direct Comparison of Cavity Ring Down Spectrometry and Isotope Ratio Mass Spectrometry for Detection of Sugar Adulteration in Honey Samples

2018 ◽  
Vol 101 (6) ◽  
pp. 1857-1863 ◽  
Author(s):  
Madhavi Mantha ◽  
John R Urban ◽  
William A Mark ◽  
Anatoly Chernyshev ◽  
Kevin M Kubachka
Keyword(s):  
Mass Spectrometry ◽  
Isotope Ratio ◽  
Low Cost ◽  
Isotope Ratio Mass Spectrometry ◽  
Cane Sugar ◽  
Technical Knowledge ◽  
Scientific Methods ◽  
High Fructose ◽  
Honey Analysis ◽  
Cavity Ring Down

Abstract In the last several years, economically motivated adulteration (EMA) of foods including honey has received increased attention. The addition of inexpensive sweeteners such as high fructose corn syrup or cane sugar to honey is still encountered despite scientific methods that can routinely detect this type of adulteration. The standard method for detection of these adulterants utilizes isotope ratio mass spectrometry (IRMS); however, this technique requires an elevated degree of technical knowledge for operation as well as a high cost for purchase and maintenance. Cavity ring down spectroscopy (CRDS) has demonstrated potential for this type of analysis and is less expensive with simpler operation. This study evaluates CRDS for the detection of low-cost sweeteners added to honey and compares the performance of CRDS to IRMS. Several honey samples were analyzed, and the advantages and limitations specific to CRDS were evaluated. Overall, the results indicate that CRDS provides a performance comparable to the benchmark technique IRMS for EMA honey analysis.

scholarly journals Mass spectrometric measurement of hydrogen isotope fractionation for the reactions of chloromethane with OH and Cl

2018 ◽  
Vol 18 (9) ◽  
pp. 6625-6635 ◽  
Author(s):  
Frank Keppler ◽  
Enno Bahlmann ◽  
Markus Greule ◽  
Heinz Friedrich Schöler ◽  
Julian Wittmer ◽  
...  
Keyword(s):  
Hydrogen Isotope ◽  
Isotope Fractionation ◽  
Isotope Effects ◽  
Isotope Analysis ◽  
Isotope Ratio Mass Spectrometry ◽  
Detailed Knowledge ◽  
Mass Spectrometric Measurement ◽  
Isotopic Analyses ◽  
Chlorine Radicals ◽  
Stable Hydrogen Isotope

Abstract. Chloromethane (CH3Cl) is an important provider of chlorine to the stratosphere but detailed knowledge of its budget is missing. Stable isotope analysis is a potentially powerful tool to constrain CH3Cl flux estimates. The largest degree of isotope fractionation is expected to occur for deuterium in CH3Cl in the hydrogen abstraction reactions with its main sink reactant tropospheric OH and its minor sink reactant Cl atoms. We determined the isotope fractionation by stable hydrogen isotope analysis of the fraction of CH3Cl remaining after reaction with hydroxyl and chlorine radicals in a 3.5 m3 Teflon smog chamber at 293 ± 1 K. We measured the stable hydrogen isotope values of the unreacted CH3Cl using compound-specific thermal conversion isotope ratio mass spectrometry. The isotope fractionations of CH3Cl for the reactions with hydroxyl and chlorine radicals were found to be -264±45 and -280±11 ‰, respectively. For comparison, we performed similar experiments using methane (CH4) as the target compound with OH and obtained a fractionation constant of -205±6 ‰ which is in good agreement with values previously reported. The observed large kinetic isotope effects are helpful when employing isotopic analyses of CH3Cl in the atmosphere to improve our knowledge of its atmospheric budget.

scholarly journals Compound Specific Carbon Isotope Analysis in Sake by LC/IRMS and Brewers’ Alcohol Proportion

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Momoka Suto ◽  
Hiroto Kawashima
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Isotope Ratio ◽  
Isotope Analysis ◽  
Isotope Ratio Mass Spectrometry ◽  
C3 Plants ◽  
C4 Plant ◽  
Compound Specific Carbon Isotope ◽  
First Time ◽  
The Relationship

AbstractSake is a traditional Japanese alcohol. Nowadays, the consumption for Sake is increasing in worldwide and its popularity is growing. However, there are act of fraudulence by additional brewers’ alcohol and sugar. Therefore, a method is needed to find illegal fraud on label. In this work, we analyzed the δ13C values of the ethanol (δ13Ceth) and glucose (δ13Cglu) in Sake by liquid chromatography combined with isotope ratio mass spectrometry for the first time. Further, we developed the criteria using δ13Ceth and δ13Cglu to check brewers’ alcohol and sugar. In addition, there are some sake categories (Ginjyo and Futsu-shu) allowed to additional brewers’ alcohol up to legally determined percentage. The experimental additions of brewers’ alcohol from a C4 plant were conducted to Junmai, as sake by C3 plants. There was a strong correlation (R = 0.98, P < 0.05) between the percentage of added brewers’ alcohol and the δ13C values. We developed the method using the relationship for calculating percentage of brewers’ alcohol for the first time and estimated the percentage for commercial sake. Further, the price of sake was found to be inversely related to the percentage of brewers’ alcohol in the sake.

scholarly journals Measurement of δ18O and δ2H of water and ethanol in wine by Off-Axis Integrated Cavity Output Spectroscopy and Isotope Ratio Mass Spectrometry

2021 ◽  
Author(s):  
Xing Wang ◽  
Henk G. Jansen ◽  
Haico Duin ◽  
Harro A. J. Meijer
Keyword(s):  
Mass Spectrometry ◽  
Isotope Ratio ◽  
Isotope Analysis ◽  
Isotope Ratio Mass Spectrometry ◽  
Growth Conditions ◽  
The Other ◽  
H Nmr ◽  
Cavity Output ◽  
Pre Treatment ◽  
Output Laser

AbstractThere are two officially approved methods for stable isotope analysis for wine authentication. One describes δ18O measurements of the wine water using Isotope Ratio Mass Spectrometry (IRMS), and the other one uses Deuterium-Nuclear Magnetic Resonance (2H-NMR) to measure the deuterium of the wine ethanol. Recently, off-axis integrated cavity output (laser) spectroscopy (OA-ICOS) has become an easier alternative to quantify wine water isotopes, thanks to the spectral contaminant identifier (SCI). We utilized an OA-ICOS analyser with SCI to measure the δ18O and δ2H of water in 27 wine samples without any pre-treatment. The OA-ICOS results reveal a wealth of information about the growth conditions of the wines, which shows the advantages to extend the official δ18O wine water method by δ2H that is obtained easily from OA-ICOS. We also performed high-temperature pyrolysis and chromium reduction combined with IRMS measurements to illustrate the “whole wine” isotope ratios. The δ18O results of OA-ICOS and IRMS show non-significant differences, but the δ2H results of both methods differ much more. As the δ2H difference between these two methods is mainly caused by ethanol, we investigated the possibility to deduce deuterium of wine ethanol from this difference. The results present large uncertainties and deviate from the obtained 2H-NMR results. The deviation is caused by the other constituents in the wine, and the uncertainty is due to the limited precision of the SCI-based correction, which need to improve to obtain the 2H values of ethanol as alternative for the 2H-NMR method.

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