scholarly journals Publisher Correction: In vivo structure of the Legionella type II secretion system by electron cryotomography

2020 ◽  
Vol 5 (4) ◽  
pp. 651-651
Author(s):  
Debnath Ghosal ◽  
Ki Woo Kim ◽  
Huaixin Zheng ◽  
Mohammed Kaplan ◽  
Hilary K. Truchan ◽  
...  
Keyword(s):  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Type Ii Secretion System ◽  
Electron Cryotomography

scholarly journals In vivo structure of the Legionella type II secretion system by electron cryotomography

2019 ◽  
Vol 4 (12) ◽  
pp. 2101-2108 ◽  
Author(s):  
Debnath Ghosal ◽  
Ki Woo Kim ◽  
Huaixin Zheng ◽  
Mohammed Kaplan ◽  
Hilary K. Truchan ◽  
...  
Keyword(s):  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Type Ii Secretion System ◽  
Electron Cryotomography

scholarly journals In vivo structure of the Legionella type II secretion system by electron cryotomography

2019 ◽  
Author(s):  
Debnath Ghosal ◽  
Ki Woo Kim ◽  
Huaixin Zheng ◽  
Mohammed Kaplan ◽  
Joseph P. Vogel ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Cell Envelope ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Type Iv ◽  
Type Ii Secretion System ◽  
Wide Range ◽  
Electron Cryotomography

AbstractThe type II secretion system (T2SS) is a multi-protein envelope-spanning assembly that translocates a wide range of virulence factors, enzymes and effectors through the outer membrane (OM) of many Gram-negative bacteria. Here, using electron cryotomography and subtomogram averaging methods, we present the first in situ structure of an intact T2SS, imaged within the human pathogen Legionella pneumophila. Although the T2SS has only limited sequence and component homology with the evolutionarily-related Type IV pilus (T4P) system, we show that their overall architectures are remarkably similar. Despite similarities, there are also differences, including for instance that the T2SS-ATPase complex is usually present but disengaged from the inner membrane, the T2SS has a much longer periplasmic vestibule, and it has a short-lived flexible pseudopilus. Placing atomic models of the components into our ECT map produced a complete architectural model of the intact T2SS that provides new insights into the structure and function of its components, its position within the cell envelope, and the interactions between its different subcomplexes. Overall, these structural results strongly support the piston model for substrate extrusion.

scholarly journals GspD, The Type II Secretion System Secretin of Leptospira, Protects Hamsters against Lethal Infection with a Virulent L. interrogans Isolate

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 759
Author(s):  
Samantha Paulina Llanos Salinas ◽  
Luz Olivia Castillo Sánchez ◽  
Giselle Castañeda Miranda ◽  
Ernesto Armando Rodríguez Reyes ◽  
Liliana Ordoñez López ◽  
...  
Keyword(s):  
Virulent Strain ◽  
Lethal Dose ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Leptospira Interrogans ◽  
Type Ii ◽  
Pathogenic Leptospira ◽  
Exact Test ◽  
Type Ii Secretion System

The wide variety of pathogenic Leptospira serovars and the weak protection offered by the available vaccines encourage the search for protective immunogens against leptospirosis. We found that the secretin GspD of the type II secretion system (T2S) of Leptospira interrogans serovar Canicola was highly conserved amongst pathogenic serovars and was expressed in vivo during infection, as shown by immunohistochemistry. Convalescent sera of hamsters, dogs, and cows showed the presence of IgG antibodies, recognizing a recombinant version of this protein expressed in Escherichia coli (rGspDLC) in Western blot assays. In a pilot vaccination study, a group of eight hamsters was immunized on days zero and 14 with 50 µg of rGspDLC mixed with Freund’s incomplete adjuvant (FIA). On day 28 of the study, 1,000 LD50 (Lethal Dose 50%) of a virulent strain of Leptospira interrogans serovar Canicola (LOCaS46) were inoculated by an intraoral submucosal route (IOSM). Seventy-five percent protection against disease (p = 0.017573, Fisher’s exact test) and 50% protection against infection were observed in this group of vaccinated hamsters. In contrast, 85% of non-vaccinated hamsters died six to nine days after the challenge. These results suggest the potential usefulness of the T2S secretin GspD of Leptospira as a protective recombinant vaccine against leptospirosis.

scholarly journals Type II Secretion System of Pseudomonas aeruginosa: In Vivo Evidence of a Significant Role in Death Due to Lung Infection

2011 ◽  
Vol 203 (10) ◽  
pp. 1369-1377 ◽  
Author(s):  
Jeevan Jyot ◽  
Viviane Balloy ◽  
Gregory Jouvion ◽  
Amrisha Verma ◽  
Lhousseine Touqui ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Significant Role ◽  
Lung Infection ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Type Ii Secretion System

scholarly journals Solution Structure of Homology Region (HR) Domain of Type II Secretion System

2012 ◽  
Vol 287 (12) ◽  
pp. 9072-9080 ◽  
Author(s):  
Shuang Gu ◽  
Geoff Kelly ◽  
Xiaohui Wang ◽  
Tom Frenkiel ◽  
Vladimir E. Shevchik ◽  
...  
Keyword(s):  
Solution Structure ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Homology Region ◽  
Type Ii Secretion System

scholarly journals Generation of Xylose-inducible promoter tools for Pseudomonas species and their use in implicating a role for the Type II secretion system protein XcpQ in inhibition of corneal epithelial wound closure

2020 ◽  
Author(s):  
Jake D. Callaghan ◽  
Nicholas A. Stella ◽  
Kara M. Lehner ◽  
Benjamin R. Treat ◽  
Kimberly M. Brothers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Closure ◽  
Inducible Promoter ◽  
Secretion System ◽  
Type Ii Secretion ◽  
Type Ii ◽  
Corneal Epithelial ◽  
E Coli ◽  
Type Ii Secretion System ◽  
Promoter System

ABSTRACTTunable control of gene expression is an invaluable tool for biological experiments. In this study, we describe a new xylose-inducible promoter system and evaluate it in both Pseudomonas aeruginosa and P. fluorescens. The Pxut promoter derived from the P. flurorescens xut operon was incorporated into a broad host-range pBBR1-based plasmid and compared to the Escherichia coli-derived PBAD promoter using gfp as a reporter. GFP-fluorescence from the Pxut promoter was inducible in both Pseudomonas species, but not in E. coli, which may facilitate cloning of toxic genes using E. coli to generate plasmids. The Pxut promoter was expressed at a lower inducer concentration than PBAD in P. fluorescens and higher gfp levels were achieved using Pxut. Flow cytometry analysis indicated that Pxut was more leaky than PBAD in the tested Pseudomonas species, but was expressed in a higher proportion of cells when induced. D-xylose did not support growth of P. aeruginosa or P. fluorescens as a sole carbon source and is less expensive than many other commonly used inducers which could facilitate large scale applications. The efficacy of this system aided in demonstrating a role for the P. aeruginosa type II secretion system gene from xcpQ in bacterial inhibition of corneal epithelial cell wound closure. This study introduces a new inducible promoter system for gene expression for use in Pseudomonas species.ImportancePseudomonas species are enormously important in human infections, biotechnology, and as a model system for interrogating basic science questions. In this study we have developed a xylose-inducible promoter system and evaluated it in P. aeruginosa and P. fluorescens and found it to be suitable for the strong induction of gene expression. Furthermore, we have demonstrated its efficacy in controlled gene expression to show that a type 2 secretion system protein from P. aeruginosa, XcpQ, is important for host-pathogen interactions in a corneal wound closure model.

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