Multiomics analysis of tolerant interaction of potato with potato virus Y

Abstract Potato virus Y (PVY) is the most economically important viral pathogen of potato worldwide. Different potato cultivars react to the pathogen differently, resulting in resistant, tolerant or disease outcome of the interaction. Here we focus on tolerant interaction between potato cv. Désirée and PVYNTN. To capture the response in its full complexity, we analyzed the dynamic changes on multiple molecular levels, including transcriptomics, sRNAomics, degradomics, proteomics and hormonomics. The analysis was complemented by the measurements of viral accumulation, photosynthetic activity and phenotypisation of the symptoms. Besides cv. Désirée we also studied its transgenic counterpart depleted for the accumulation of salicylic acid (NahG-Désirée). This multiomics analysis provides better insights into the mechanisms leading to tolerant response of potato to viral infection and can be used as a base in further studies of plant immunity regulation.

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Salicylic acid perturbs sRNA-gibberellin regulatory network in immune response of potato to Potato virus Y infection

10.1101/130757 ◽

2017 ◽

Author(s):

Maja Križnik ◽

Marko Petek ◽

David Dobnik ◽

Živa Ramšak ◽

Špela Baebler ◽

...

Keyword(s):

Salicylic Acid ◽

Potato Virus ◽

Regulatory Network ◽

Potato Virus Y ◽

Severe Disease ◽

Functional Relation ◽

Gibberellin Biosynthesis ◽

Post Inoculation ◽

Viral Pathogen ◽

Gibberellin Signaling

AbstractPotato virus Y is the most economically important potato viral pathogen. We aimed at unraveling the roles of small RNAs (sRNAs) in the complex immune signaling network controlling the establishment of tolerant response of potato cv. Désirée to the virus. We constructed a sRNA regulatory network connecting sRNAs and their targets to link sRNA level responses to physiological processes. We discovered an interesting novel sRNAs-gibberellin regulatory circuit being activated as early as 3 days post inoculation before viral multiplication can be detected. Increased levels of miR167 and phasiRNA931 were reflected in decreased levels of transcripts involved in gibberellin biosynthesis. Moreover, decreased concentration of gibberellin confirmed this regulation. The functional relation between lower activity of gibberellin signaling and reduced disease severity was previously confirmed in Arabidopsis-virus interaction using knockout mutants. We further showed that this regulation is salicylic acid-dependent as the response of sRNA network was attenuated in salicylic acid-depleted transgenic counterpart NahG-Désirée expressing severe disease symptoms. Besides downregulation of gibberellin signaling, regulation of several other parts of sRNA network in tolerant Désirée revealed similarities to responses observed in mutualistic symbiotic interactions. The intertwining of different regulatory networks revealed shows how developmental signaling, disease symptom development and stress signaling can be balanced.

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Erratum to: Bacterially Expressed Double-Stranded RNAs against Hot-Spot Sequences of Tobacco Mosaic Virus or Potato Virus Y Genome Have Different Ability to Protect Tobacco from Viral Infection

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-010-8999-8 ◽

2010 ◽

Vol 162 (5) ◽

pp. 1517-1517 ◽

Cited By ~ 6

Author(s):

Guo-Hua Yin ◽

Zhao-Nan Sun ◽

Yun-Zhi Song ◽

Hai-Long An ◽

Chang-Xiang Zhu ◽

...

Keyword(s):

Viral Infection ◽

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Potato Virus ◽

Potato Virus Y ◽

Hot Spot

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MONITORING STUDIES OF VIRAL DISEASES IN POTATOES PLANTINGS OF UKRAINIAN MARSHY WOODLANDS

Agriciltural microbiology ◽

10.35868/1997-3004.15.140-149 ◽

2012 ◽

Vol 15 ◽

pp. 140-149

Author(s):

O.A. Dmitruk ◽

Yu.A. Dmitruk ◽

T.A. Bova ◽

A.V. Pirog ◽

L.P. Kolomiets

Keyword(s):

Viral Infection ◽

Potato Virus ◽

Potato Virus Y ◽

Viral Diseases ◽

Latent Infections ◽

Viral Control ◽

Laboratory Methods ◽

Seed Material ◽

Original Seed ◽

Efficient Protection

The examinations of potatoes original seed nursery in north regions of Ukrainian marshy woodlands in 2012 (47 sorts) were performed. It was revealed that spreading of viral infection and diseases symptoms onset on potatoes cultivars increases with the number of field reproductions from 8,1 % (tube plants – seedlings) to 77–100 %. The wide-spreading of latent infections was observed – 57 % of tested samples. Potato virus М prevails in plantings as monoinfection (36,2 %) or in complex with other mosaic viruses (99 %), Potato virus Y was detected in 38,3 % of cultivars, with domination of necrotic isolates. The need of efficient protection and constant viral control of seed material with the use of laboratory methods and modern diagnostic facilities is emphasized.

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Salicylic Acid Suppresses Potato virus Y Isolate N:O-Induced Symptoms in Tobacco Plants

Phytopathology ◽

10.1094/phyto-96-0255 ◽

2006 ◽

Vol 96 (3) ◽

pp. 255-263 ◽

Cited By ~ 27

Author(s):

Xianzhou Nie

Keyword(s):

Salicylic Acid ◽

Potato Virus ◽

Systemic Acquired Resistance ◽

Potato Virus Y ◽

Early Stage ◽

Acquired Resistance ◽

Partial Recovery ◽

Pr Genes ◽

Tobacco Plants ◽

Systemic Development

The effects of salicylic acid (SA) and 1-aminocyclopropane-1-carboxylic acid (ACC) on the systemic development of symptoms induced by a severe isolate of Potato virus Y group N:O (PVYN:O) in tobacco were investigated. Upon inoculation, the systemic development of symptoms in tobacco plants could be divided into three stages: virus incubation stage, rapid symptom-progress stage, and partial recovery and symptom-shifting stage. Treatment of seedlings with SA delayed the virus-induced necrosis in stems by 1 to 2 days. SA, not ACC, also significantly suppressed the symptom severity in stems. However, neither SA nor ACC treatment affected the partial recovery phenotype exhibited in the latterly emerged upper parts of the plants. Further analysis indicated that the accumulation of PVY was retarded by SA at the early stage of infection, and the effects were more profound in stems than leaves. Peroxidase (POX) activity and pathogenesis-related (PR) genes PR-1a and PR-1b were enhanced by PVY infection. SA not only increased POX activity in stems and PR genes in stems and leaves of mock-inoculated plants, but also elevated the activity of POX in both leaves and stems and the expression of PR-1a in leaves of PVY-infected plants. Together, the results suggest that systemic acquired resistance plays a key role in suppressing PVYN:O-induced symptom development through SA-mediated and ethylene-independent pathways. The symptom suppression was correlated with reduced replication/ accumulation of virus at the early stage of infection. The results also suggest that neither SA nor ethylene plays a role in the recovery phenotype.

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