scholarly journals Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ming Wang ◽  
Zhaolin Sun ◽  
Tian Yu ◽  
Fangrong Ding ◽  
Ling Li ◽  
...  
MedChemComm ◽  
2015 ◽  
Vol 6 (3) ◽  
pp. 486-491 ◽  
Author(s):  
Chun Loong Ho ◽  
In Young Hwang ◽  
Kathy Loh ◽  
Matthew Wook Chang

An improved method of recombinant human lactoferrin (hLF) expression in rich culture medium is demonstrated using macroporous microencapsulation.


2002 ◽  
Vol 20 (5) ◽  
pp. 484-487 ◽  
Author(s):  
Patrick H.C. van Berkel ◽  
Mick M. Welling ◽  
Marlieke Geerts ◽  
Harry A. van Veen ◽  
Bep Ravensbergen ◽  
...  

PLoS ONE ◽  
2008 ◽  
Vol 3 (10) ◽  
pp. e3453 ◽  
Author(s):  
Penghua Yang ◽  
Jianwu Wang ◽  
Guochun Gong ◽  
Xiuzhu Sun ◽  
Ran Zhang ◽  
...  

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Dan Lu ◽  
Shen Liu ◽  
Fangrong Ding ◽  
Haiping Wang ◽  
Jing Li ◽  
...  

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2860 ◽  
Author(s):  
Da Li ◽  
Fangling Ji ◽  
Chundong Huang ◽  
Lingyun Jia

Nanobodies (VHHs) overcome many of the drawbacks of conventional antibodies, and the related technologies represent state-of-the-art and advanced applications in scientific research, pharmaceuticals, and therapies. In terms of productivity and economic cost, the cytoplasmic expression of VHHs in Escherichia coli (E. coli) is a good process for their recombinant production. The cytoplasmic environment of the host is critical to the affinity and stability of the recombinant VHHs in soluble form, yet the effects have not been studied. For this purpose, recombinant anti-β2 microglobulin VHHs were constructed and expressed in four commercialized E. coli hosts, including BL21 (DE3), Rosetta-gami B (DE3) pLysS, Origami 2 (DE3) and SHuffle T7 Express. The results showed that anti-β2 microglobulin (β2MG) VHHs expressed in different hosts exhibited distinctive differences in the affinity and structural characteristics. The VHHs expressed in Rosetta-gami B (DE3) pLysS possessed not only the greatest affinity of (equilibrium dissociation constant) KD = 4.68 × 10−8 M but also the highest yields compared with the VHHs expressed in BL21 (DE3), Origami 2 (DE3) and SHuffle T7 Express. In addition, the VHHs expressed in Rosetta-gami B (DE3) pLysS were more stable than the VHHs expressed in the rest three hosts. Thus far, we have successfully realized the high expression of the active and robust anti-β2MG VHHs in Rosetta-gami B (DE3) pLysS. The underlying principle of our study is able to guide the expression strategies of nanobodies on the context of industrial large-scale production.


2013 ◽  
Vol 33 (1) ◽  
pp. 23-37 ◽  
Author(s):  
Norman Oliva ◽  
Prabhjit Chadha-Mohanty ◽  
Susanna Poletti ◽  
Editha Abrigo ◽  
Genelou Atienza ◽  
...  

1993 ◽  
Vol 32 (1) ◽  
pp. 129-131
Author(s):  
Naureen Talha

The literature on female labour in Third World countries has become quite extensive. India, being comparatively more advanced industrially, and in view of its size and population, presents a pictures of multiplicity of problems which face the female labour market. However, the author has also included Mexico in this analytical study. It is interesting to see the characteristics of developing industrialisation in two different societies: the Indian society, which is conservative, and the Mexican society, which is progressive. In the first chapter of the book, the author explains that he is not concerned with the process of industrialisation and female labour employed at different levels of work, but that he is interested in forms of production and women's employment in large-scale production, petty commodity production, marginal small production, and self-employment in the informal sector. It is only by analysis of these forms that the picture of females having a lower status is understood in its social and political setting.


2018 ◽  
Vol 15 (4) ◽  
pp. 572-575 ◽  
Author(s):  
Ponnusamy Kannan ◽  
Samuel I.D. Presley ◽  
Pallikondaperumal Shanmugasundaram ◽  
Nagapillai Prakash ◽  
Deivanayagam Easwaramoorthy

Aim and Objective: Itopride is a prokinetic agent used for treating conditions like non-ulcer dyspepsia. Itopride is administered as its hydrochloride salt. Trimethobenzamide is used for treating nausea and vomiting and administered as its hydrochloride salt. The aim is to develop a novel and environmental friendly method for large-scale production of itopride and trimethobenzamide. Materials and Methods: Itopride and trimethobenzamide can be prepared from a common intermediate 4- (dimethylaminoethoxy) benzyl amine. The intermediate is prepared from one pot synthesis using Phyrdroxybenzaldehye and zinc dust and further reaction of the intermediate with substituted methoxy benzoic acid along with boric acid and PEG gives itopride and trimethobenzamide. Results: The intermediate 4-(dimethylaminoethoxy) benzylamine is prepared by treating p-hydroxybenzaldehyde and 2-dimethylaminoethyl chloride. The aldehyde formed is treated with hydroxylamine hydrochloride. The intermediate is confirmed by NMR and the purity is analysed by HPLC. Conclusion: Both itopride and trimethobenzamide were successfully synthesized by this method. The developed method is environmental friendly, economical for large-scale production with good yield and purity.


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