scholarly journals Palm Fruit Bioactives modulate human astrocyte activity in vitro altering the cytokine secretome reducing levels of TNFα, RANTES and IP-10

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Robert P. Weinberg ◽  
Vera V. Koledova ◽  
Kirsten Schneider ◽  
T. G. Sambandan ◽  
Adlai Grayson ◽  
...  
Keyword(s):  
Palm Fruit ◽  
Human Astrocyte

scholarly journals Potentials and Safety of Date Palm Fruit against Diabetes: A Critical Review

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1557
Author(s):  
Md Al-Tareq Mia ◽  
Md Golam Mosaib ◽  
Md Ibrahim Khalil ◽  
Md Asiful Islam ◽  
Siew Hua Gan
Keyword(s):  
Date Palm ◽  
Antioxidant Activities ◽  
Human Study ◽  
Insulin Receptors ◽  
Β Cells ◽  
Palm Fruit ◽  
Good Potential ◽  
Palm Fruits

Diabetes is a chronic metabolic disorder triggered by disturbances in carbohydrate, protein, and lipid metabolisms, where either reduced secretion or sensitivity of insulin is observed coupled with poor glucose control. Date palm fruits are one of the fruits reported to have good potential in diabetes treatment due to its presence of polyphenols exerting strong antioxidant activities. Other possible mechanisms of action include the polyphenolic compounds, which can inhibit enzymes like α-amylase and α-glucosidase. Flavonoids in dates can stimulate β-cells by increasing the number of islets and β-cells, recovering endocrine pancreatic tissues, reducing β-cell apoptosis, activating insulin receptors following the increase in insulin secretion, in addition to improving diabetes-induced complications. In this review, the in vitro, in vivo, and human study-based evidence of date palm as an anti-diabetic fruit is summarised.

scholarly journals Differentiated HASTR/ci35 cells: A promising in vitro human astrocyte model for facilitating CNS drug development studies

2018 ◽  
Vol 137 (4) ◽  
pp. 350-358 ◽  
Author(s):  
Keita Kitamura ◽  
Ryo Ito ◽  
Kenta Umehara ◽  
Hanae Morio ◽  
Kosuke Saito ◽  
...  
Keyword(s):  
Drug Development ◽  
Development Studies ◽  
Human Astrocyte

scholarly journals Extracts of Jordanian Date Palm Fruit (Phoenix Dactylifera L.) Inhibit Human Mammary Adenocarcinoma (MCF-7) Cells In Vitro by Inducing Cell Viability

2021 ◽  
Vol 9 (2) ◽  
pp. 423-430
Author(s):  
Hiba F. Al-Sayyed ◽  
Luay F. Abu-Qatouseh ◽  
Mohammed Malkawy ◽  
Suzan Al-Wawi ◽  
Mohammad Al Kafaween
Keyword(s):  
Cell Viability ◽  
Anticancer Agents ◽  
Mammary Cancer ◽  
Date Palm ◽  
Water Extract ◽  
Mammary Adenocarcinoma ◽  
Palm Fruit ◽  
Maturity Stages ◽  
Mcf 7

Worldwide, and in Jordan specifically, date palm fruit production has been steadily increasing. Recently, various dates extracts have been used functionally as antioxidants or anticancer agents. In addition, Jordanian date palm fruit of Barhi variety at two maturity stages (Rutab) and (Tamr) inhibited chemically-induced mammary cancer in animal model. The aim of this study is to strengthen the scientific evidence on the effect of dates on mammary cancer via assessing the effect of different concentrations of water extracts of three varieties of dates grown in Jordan (Barhi, Belle Huwaimil, and Medjool) at two maturity stages (Rutab and Tamr) on the viability of MCF-7 breast cancer cell line. The effects of different extracts on MCF7 inhibition/proliferation was analyzed using MTT assay. Percentage of inhibition was calculated. The most effective concentration was 100 mg/ml for all varieties. At this concentration, Medjool variety at the Tamr stage exhibited the highest inhibition. At the 12.5 and 25 mg/ml concentrations of the extracts, the most effective date palm fruit varieties were Belle Huwaimil and Barhi at the Tamr stage respectively. At lower concentrations (3.125 and 6.25 mg/ml), Medjool at the Tamr stage and Belle Huwaimil respectively were the most effective extracts were Barhi at the Tamr and Rutab stages were the least effective (P=0.000**). It is concluded that water extract of palm fruit reduced MCF-7 cell viability.

scholarly journals Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 735
Author(s):  
Hany Ezzat Khalil ◽  
Nashi K. Alqahtani ◽  
Hossam M. Darrag ◽  
Hairul-Islam Mohamed Ibrahim ◽  
Promise M. Emeka ◽  
...  
Keyword(s):  
Particle Size ◽  
Serum Protein ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Qualitative Assessment ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Phenolic ◽  
Palm Fruit

Date palm fruit (Phoenix dactylifera) is reputed to have numerous biological activities, including anticancer properties. To utilize the great fortune of this fruit, the current study aimed to maximize its pharmacological activity. Date palm extract (DPE) of Khalas cultivar was obtained in powder form and then was formulated into nanoemulsion (NE). The optimized DPE-NE was formulated along with its naked counterpart followed by studying their physical and chemical properties. A qualitative assessment of total serum protein associated with the surface of formulations was implemented. Studies for the in vitro release of DPE from developed NE before and after incubation with serum were investigated. Eventually, an MTT assay was conducted. Total phenolic and flavonoid contents were 22.89 ± 0.013 mg GAE/g of dry DPE and 9.90 ± 0.03 mg QE/g of dry DPE, respectively. Homogenous NE formulations were attained with appropriate particle size and viscosity that could be administered intravenously. The optimized PEGylated NE exhibited a proper particle size, PDI, and zeta potential. Total serum protein adsorbed on PEG-NE surface was significantly low. The release of the drug through in vitro study was effectively extended for 24 h. Ultimately; PEGylated NE of DPE attained significant inhibition for cancer cell viability with IC50 values of 18.6 ± 2.4 and 13.5 ± 1.8 µg/mL for MCF-7 and HepG2 cell lines, respectively. PEGylated NE of DPE of Khalas cultivar will open the gate for future adjuvants for cancer therapy.

scholarly journals ANTIOXIDANT ACTIVITY AND INHIBITION OF LIPOXYGENASE ACTIVITY ETHANOL EXTRACT OF ENDOSPERM ARENGA PINNATA (WURMB) MERR.

2017 ◽  
Vol 10 (17) ◽  
pp. 76
Author(s):  
Berna Elya ◽  
Nuraini Puspitasari ◽  
Annisa Chairani Sudarmin
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Test ◽  
Lipoxygenase Activity ◽  
Palm Fruit ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: The purpose of this study is to determine the antioxidant activity and the potential inhibition of lipoxygenase activity from sugar palm fruit.Methods: Antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Inhibition of lipoxygenase activity was performed in vitro. Sugar palm fruit was macerated with 95% ethanol.Results: The results showed that sugar palm fruit ethanol extract has antioxidant activity when using DPPH method with EC50 of 141.3929 μg/mL and the FRAP method with EC50 of 60.2083 μg/mL. Inhibition test of lipoxygenase activity showed inhibitory concentration 50% value of 71.376 μg/mL.Conclusions: Ethanol extract of endosperm Arenga pinnata has antioxidant activity, as determined using the DPPH method with EC50of 141.3929 μg/mL and the FRAP method with EC50 of 60.2083 μg/mL.

Traumatic injury elicits JNK-mediated human astrocyte retraction in vitro

Neuroscience ◽  
2014 ◽  
Vol 274 ◽  
pp. 1-10 ◽  
Author(s):  
C. Augustine ◽  
G. Cepinskas ◽  
D.D. Fraser
Keyword(s):  
Traumatic Injury ◽  
Human Astrocyte

scholarly journals Laquinimod Modulates Human Astrocyte Function and Dampens Astrocyte-Induced Neurotoxicity during Inflammation

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5403
Author(s):  
Emanuela Colombo ◽  
Rosaria Pascente ◽  
Daniela Triolo ◽  
Claudia Bassani ◽  
Anthea De Angelis ◽  
...  
Keyword(s):  
Inflammatory Mediator ◽  
Nuclear Translocation ◽  
Suitable Model ◽  
Spinal Neurons ◽  
Human Astrocyte ◽  
Aryl Hydrocarbon ◽  
Human Astrocytes ◽  
Pharmacological Targets ◽  
Inflammatory Milieu

Astrocytes greatly participate to inflammatory and neurotoxic reactions occurring in neurodegenerative diseases and are valuable pharmacological targets to support neuroprotection. Here we used human astrocytes generated from reprogrammed fibroblasts as a cellular model to study the effect of the compound Laquinimod and its active metabolite de-Laquinimod on astrocyte functions and the astrocyte–neuron interaction. We show that human iAstrocytes expressed the receptor for the inflammatory mediator IL1 and responded to it via nuclear translocation of NFκB, an event that did not occur if cells were treated with Laquinimod, indicating a direct anti-inflammatory activity of the drug on the human astrocyte. Similarly, while exposure to IL1 downregulated glial glutamate transporters GLAST and GLT1, treatment with Laquinimod supported maintenance of physiological levels of these proteins despite the inflammatory milieu. Laquinimod also induced nuclear translocation of the aryl hydrocarbon receptor (AHR), suggesting that drug action was mediated by activation of the AHR pathway. However, the drug was effective despite AHR inhibition via CH223191, indicating that AHR signaling in the astrocyte is dispensable for drug responses. Finally, in vitro experiments with rat spinal neurons showed that laquinimod did not exert neuroprotection directly on the neuron but dampened astrocyte-induced neurodegeneration. Our findings indicate that fibroblast-derived human astrocytes represent a suitable model to study astrocyte–neuron crosstalk and demonstrate indirect, partial neuroprotective efficacy for laquinimod.

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