scholarly journals ANTIOXIDANT ACTIVITY AND INHIBITION OF LIPOXYGENASE ACTIVITY ETHANOL EXTRACT OF ENDOSPERM ARENGA PINNATA (WURMB) MERR.

2017 ◽  
Vol 10 (17) ◽  
pp. 76
Author(s):  
Berna Elya ◽  
Nuraini Puspitasari ◽  
Annisa Chairani Sudarmin
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Test ◽  
Lipoxygenase Activity ◽  
Palm Fruit ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: The purpose of this study is to determine the antioxidant activity and the potential inhibition of lipoxygenase activity from sugar palm fruit.Methods: Antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Inhibition of lipoxygenase activity was performed in vitro. Sugar palm fruit was macerated with 95% ethanol.Results: The results showed that sugar palm fruit ethanol extract has antioxidant activity when using DPPH method with EC50 of 141.3929 μg/mL and the FRAP method with EC50 of 60.2083 μg/mL. Inhibition test of lipoxygenase activity showed inhibitory concentration 50% value of 71.376 μg/mL.Conclusions: Ethanol extract of endosperm Arenga pinnata has antioxidant activity, as determined using the DPPH method with EC50of 141.3929 μg/mL and the FRAP method with EC50 of 60.2083 μg/mL.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

Antioxidant Activity Test Ethanol Extract of Lontar Fruits (Borassus flabellifer L.) and Ginger Bud (Etlingera elatior (Jack.) R.M. Sm) Using Ferric Reducing Antioxidant Power Method

2019 ◽  
Vol 967 ◽  
pp. 34-37
Author(s):  
A. Muflihunna ◽  
Rahmawati ◽  
Mamat Pratama ◽  
Andi Mu'nisa ◽  
Astuti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Power Method ◽  
Fruit Peel ◽  
Palm Fruit ◽  
Antioxidant Power ◽  
Average Value ◽  
Fruit Skin ◽  
Ferric Reducing Antioxidant Power ◽  
Etlingera Elatior

This study aims to examine the activity of the antioxidant activity of ethanol extract of lontar fruit peel (Borassus flabellifer l.) and ginger bud (Etlingera elatior (Jack.) R.M. SM) by using the ferric reducing antioxidant power method. Extraction was carried out by maceration method using 70% ethanol. The extracts were analyzed quantitatively with the addition of FRAP reagents measured by UV-Vis spectrophotometer analyzed at a wavelength of 752.29 nm in the ginger bud fruit peel and 727.05 nm for palm fruit skin using a quartetine as a comparison. Based on data analysis, the average value of the three antioxidant activity of the replication was 2.482 mg QE / g Extract on the skin of ginger bud fruit and 99.846 mgQE / g extract for lontar fruit skin. The conclusion shows that both the ginger bud fruit skin and lontar fruit skin have antioxidant activity.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

2021 ◽  
Vol 332 ◽  
pp. 08005
Author(s):  
Aminah Dalimunthe ◽  
Dewi Pertiwi ◽  
Mahatir Muhammad ◽  
Denny Satria
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Litsea Cubeba ◽  
Ferric Reducing Antioxidant Power ◽  
Phenolic And Flavonoid

Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

scholarly journals Evaluation of In Vitro Antioxidant and Antidiabetic Activities of Aristolochia longa Extracts

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Nasreddine El Omari ◽  
Karima Sayah ◽  
Saad Fettach ◽  
Omar El Blidi ◽  
Abdelhakim Bouyahya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Inhibitory Effect ◽  
Phytochemical Analysis ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Therapeutic Tools

Oxidative stress plays a major role in diabetic physiopathology; hence, the interest of using natural antioxidants as therapeutic tools exists. The aim of this study was the evaluation of in vitro antioxidant activity and inhibitory potential of organic extracts from Aristolochia longa roots against key enzymes linked to hyperglycemia. Antioxidant activity was performed using 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals and ferric reducing/antioxidant power (FRAP) methods. The α-Glucosidase and β-Galactosidase inhibitory activities were investigated using an in vitro model. Moreover, phytochemical analysis of tested extracts was carried out. The aqueous fraction of this herb exhibited the highest antioxidant activity for both DPPH and ABTS methods, IC50=125.40±2.40 μg/mL and IC50=65.23±2.49 μg/mL, respectively. However, the ethyl acetate fraction possessed the strongest inhibitory effect towards α-Glucosidase (IC50=1.112±0.026 mg/mL). Furthermore, the result showed high levels of phenolic content. The results showed that this plant could be a significant source of medically important natural compounds.

scholarly journals Changes of antioxidant activity in honey after heat treatment

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 601-606 ◽  
Author(s):  
G. Šarić ◽  
K. Marković ◽  
D. Vukičević ◽  
E. Lež ◽  
M. Hruškar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Significant Difference ◽  
Acacia Honey ◽  
Different Temperatures ◽  
Ferric Reducing Antioxidant Power

We determined how the antioxidant activity and total phenolic content of honey changed after being subjected to a high temperature. Antioxidant activity was determined using two methods – FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Total phenolic content was determined by modified Folin-Ciocalteu method. The research was conducted on 31 samples of acacia honey and 8 samples of chestnut honey. All measurements were done at two temperatures – at 23°C (room temperature) and after 5 min of heating at 95°C. The obtained results show uneven changes of antioxidant activity and total phenolic content among individual samples, i.e. in some samples antioxidant activity decreased after heating, while in others it increased. The same applies to the total phenolic content. Statistical analysis of the results (t-test) showed no statistically significant differences between the results measured at two different temperatures (P > 0.05) in all three methods used, and in both types of honey. The only statistically significant difference (P < 0.05) was observed when using DPPH method in acacia honey.

scholarly journals From the Field to the Pot: Phytochemical and Functional Analyses of Calendula officinalis L. Flower for Incorporation in an Organic Yogurt

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 559 ◽  
Author(s):  
Bragueto Escher ◽  
Cardoso Borges ◽  
Sousa Santos ◽  
Mendanha Cruz ◽  
Boscacci Marques ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Calendula Officinalis ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Functional Analyses ◽  
Calendula Officinalis L

Edible flowers have been used as ingredients because of their biological activities, taste, and overall appearance. This research was aimed to characterize the chemical composition and in vitro antioxidant activity of the marigold flower (Calendula officinalis L.) extracted with different proportions of water and ethyl alcohol, and the lyophilized extract with higher content of antioxidant compounds was incorporated into an organic yogurt. Results showed that the hydroalcoholic extract (50:50 v/v) presented the highest total phenolic content (TPC), flavonoids, and antioxidant activity (ferric reducing antioxidant power (FRAP), total reducing capacity (TRC), and Cu2+/Fe2+ chelating ability). Phenolic acids and flavonoids were quantified in the extract by LC-DAD, while 19 compounds were tentatively identified by ESI-MS/MS. The lyophilized marigold extract (LME) also inhibited 12% of Wistar rat’s brain lipid oxidation in vitro, inhibited α-amylase, and α-glucosidase activities, but showed no cytotoxicity towards cancerous cells (HCT8 and A549). However, marigold flower extract protected human erythrocytes against mechanical stress. When added into an organic yogurt model (0 to 1.5%), LME increased TPC and antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH) and TRC), and the sensory analysis showed that the organic yogurt had an acceptance of 80.4%. Our results show that the use of LME may be a technological strategy to increase the content of bioactive compounds in yogurts.

scholarly journals Daya Reduksi Ekstrak Etanol Biji Aquilaria microcarpa, Aquilaria malaccensis, dan Aquilaria beccariana Terhadap Ion Ferri (Fe3+) dengan Metode FRAP (Ferric Reducing Antioxidant Power)

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Liling Triyasmono ◽  
Beny Rahmanto ◽  
Wawan Halwany ◽  
Fajar Lestari ◽  
Muhammad Ikhwan Rizki ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Key Words ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Ferric Ion ◽  
Dry Powder ◽  
Iron Compounds ◽  
Antioxidant Power ◽  
Aquilaria Malaccensis ◽  
Ferric Reducing Antioxidant Power

ABSTRAK Daya reduksi merupakan salah satu indikator potensi aktivitas suatu senyawa sebagai antioksidan. Penelitian ini bertujuan untuk menentukan daya reduksi ekstrak etanol biji Aquilaria microcarpa, Aquilaria malaccensis, dan Aquilaria beccariana terhadap Ion Ferri (Fe3+). Serbuk kering biji A. microcarpa, A. malaccensis, dan A. beccariana dimaserasi menggunakan etanol 70%. Daya reduksi ditentukan dengan metode FRAP (ferric reducing antioxidant power) yang didasarkan atas kemampuan senyawa dalam mereduksi senyawa besi(III)-tripiridil-triazin menjadi besi(II)-tripiridil triazin pada pH 3,6. Absorbansi diukur menggunakan spektrofotometer UV-Vis pada panjang gelombang 598 nm. Hasil penelitian menunjukkan ekstrak etanol biji A. microcarpa, A. malaccensis, dan A. beccariana mempunyai daya reduksi berturut-turut sebesar 6,39±1,58; 119,95±28,04; dan 62,12±6,57 µM ekivalen troloks/g ekstrak. Kata kunci: biji, Aquilaria microcarpa, Aquilaria malaccensis, Aquilaria beccariana, FRAP ABSTRACT Reducing power is one indicator of potential antioxidant activity of a compound. This study aims to determine the reduction power of the ethanol extract of the seeds of Aquilaria microcarpa, Aquilaria malaccensis, and Aquilaria beccariana against Ferric ion (Fe3+). Dry powder of A. microcarpa, A. malaccensis, and A. beccariana seeds was macerated using 70% ethanol. Reducing power determined using FRAP (ferric reducing antioxidant power) that is based on the ability of the compounds in reducing iron compounds (III) -tripiridil-triazine to iron (II) -tripiridil triazine at pH 3.6. The absorbance was measured using a UV-Vis spectrophotometer at a wavelength of 598 nm. The results showed ethanol extract of seeds of A. microcarpa, A. malaccensis, and A. beccariana have reducing power of 6.39 ± 1.58; 119.95 ± 28.04; and 62.12 ± 6.57 µM troloks equivalents / g extract respectively. Key words: seeds, Aquilaria microcarpa, Aquilaria malaccensis, Aquilaria beccariana, FRAP

scholarly journals EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

2021 ◽  
Vol 6 (8) ◽  
pp. 73-77
Author(s):  
G.SAI SRUTHI ◽  
K. SPANDANA ◽  
RAMANJANEYULU K ◽  
HIMABINDHU J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

scholarly journals Kandungan Fitokimia, Potensi Antibakteri dan Antioksidan Hasil Ekstraksi Caulerpa racemosa dengan Pelarut Berbeda

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Endar Marraskuranto ◽  
Muhammad Nursid ◽  
Swestri Utami ◽  
Iriani Setyaningsih ◽  
Kustiariyah Tarman
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Caulerpa Racemosa ◽  
Antioxidant Power ◽  
E Coli ◽  
Antibacterial Assay ◽  
Ferric Reducing Antioxidant Power

Caulerpa racemosa merupakan rumput laut hijau yang mengandung senyawa aktif yang berpotensi sebagai antibakteri dan antioksidan. Perbedaan profil fitokimia dan bioaktivitas suatu ekstrak dipengaruhi oleh kepolaran pelarut ekstraksi. Penelitian ini bertujuan untuk mempelajari profil fitokimia, aktivitas antibakteri dan antioksidan ekstrak C. racemosa. Ekstrak diperoleh dari sampel segar C. racemosa yang dimaserasi menggunakan pelarut n-heksana, etil asetat, dan metanol. Uji antibakteri dilakukan dengan metode Resazurin Microtitter Assay (REMA) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Aktivitas antioksidan diuji menggunakan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH) dan Ferric Reducing Antioxidant Power (FRAP). Hasil penapisan fitokimia memperlihatkan bahwa ekstrak n-heksana mengandung alkaloid dan triterpenoid, sedangkan ekstrak etil asetat dan metanol mengandung alkaloid, flavonoid, tanin, fenol, dan steroid. Aktivitas antibakteri terbaik ditunjukkan oleh ekstrak etil asetat dengan nilai Minimum Inhibitory Concentration (MIC) terhadap E. coli sebesar 250 mg/mL. Ekstrak etil asetat C. racemosa juga memperlihatkan aktivitas antioksidan terbaik dengan nilai IC50 DPPH sebesar 110,7 mg/mL dan nilai FRAP sebesar 96,68 mmol Fe (II)/g. ABSTRACTCaulerpa racemosa is a green seaweed that contains active compounds that are potential for antibacterial and antioxidant. The phytochemical constituents and bioactivity of an extract depend on the extraction solvent polarity. This study aimed to determine the phytochemical profiles, antibacterial and antioxidant activity of C. racemosa extracts. Extracts were obtained from the fresh C. racemosa samples that were macerated with n-hexane, ethyl acetate, and methanol. Antibacterial assay was tested by Resazurin Microtiter Assay (REMA) method against Escherichia coli and Staphylococcus aureus. Antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) method. Phytochemical screening showed that n-hexane extract contained alkaloid and triterpenoid, while ethyl acetate and methanol extracts contained alkaloids, flavonoids, tannins, phenols, and steroids. The most active extract in the antibacterial assay was ethyl acetate extract with a Minimum Inhibitory Concentration (MIC) value of 250 mg/mL against E. coli. Ethyl acetate extract of C. racemosa also showed the best antioxidant activity where the IC50 of DPPH value was 110.7 mg/mL and the FRAP value was 96.68 mmol Fe(II)/g.

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