scholarly journals Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Wei Hou ◽  
Yangyang Li ◽  
Wenjie Kang ◽  
Xin Wang ◽  
Xuping Wu ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Porcine Epidemic Diarrhea Virus ◽  
Single Particle Tracking ◽  
Time Analysis ◽  
Diarrhea Virus ◽  
Real Time Analysis ◽  
Porcine Epidemic Diarrhea

scholarly journals Single-Particle Tracking Porcine Epidemic Diarrhea Virus Moving along Microtubules in Living Cells

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 124
Author(s):  
Yangyang Li ◽  
Wei Hou ◽  
Jian Wang ◽  
Fei Liu
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Porcine Epidemic Diarrhea Virus ◽  
Living Cells ◽  
Single Particle Tracking ◽  
Microtubule Organizing Center ◽  
Swine Industry ◽  
Diarrhea Virus ◽  
Organizing Center ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused severe damage to the swine industry. Although viruses are believed to hijack the microtubule-based transport system, the exact manner of PEDV moving along microtubules has not been fully characterized. In this study, PEDV was labeled with quantum dots which have great brightness and photostability. By using quantum dot-labeled PEDV and single-particle tracking, we were able to systematically dissect the dynamic behaviors of PEDV moving along the microtubules in living cells. We found that PEDVs maintained a restricted motion mode with a relatively stable speed in the cell membrane region while displaying a slow–fast–slow velocity pattern with different motion modes in the cell cytoplasm region and near the microtubule-organizing center. The return movements of small amounts of PEDVs were also observed in living cells. Collectively, our work is crucial for understanding the movement of PEDV in living cells; the proposed work also provides important references for further analysis and studies of the infection mechanism of PEDV.

pH-Mediated nanoparticle dynamics in hydrogel nanocomposites

Soft Matter ◽  
2021 ◽  
Author(s):  
Katie A. Rose ◽  
Daeyeon Lee ◽  
Russell J. Composto
Keyword(s):  
Quantum Dot ◽  
Ethylene Glycol ◽  
Particle Tracking ◽  
Single Particle ◽  
Silica Particles ◽  
Single Particle Tracking ◽  
Poly Ethylene Glycol ◽  
Hydrogel Nanocomposites ◽  
Poly Ethylene ◽  
Nanoparticle Dynamics

The effect of static silica particles on the dynamics of quantum dot (QD) nanoparticles grafted with a poly(ethylene glycol) (PEG) brush in hydrogel nanocomposites is investigated using single particle tracking (SPT).

scholarly journals Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2826 ◽  
Author(s):  
Shangguo Hou ◽  
Courtney Johnson ◽  
Kevin Welsher
Keyword(s):  
Fluorescence Spectroscopy ◽  
Real Time ◽  
Temporal Resolution ◽  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Single Molecule Fluorescence ◽  
Single Molecule Fluorescence Spectroscopy ◽  
Active Feedback

Single molecule fluorescence spectroscopy has been largely implemented using methods which require tethering of molecules to a substrate in order to make high temporal resolution measurements. However, the act of tethering a molecule requires that the molecule be removed from its environment. This is especially perturbative when measuring biomolecules such as enzymes, which may rely on the non-equilibrium and crowded cellular environment for normal function. A method which may be able to un-tether single molecule fluorescence spectroscopy is real-time 3D single particle tracking (RT-3D-SPT). RT-3D-SPT uses active feedback to effectively lock-on to freely diffusing particles so they can be measured continuously with up to photon-limited temporal resolution over large axial ranges. This review gives an overview of the various active feedback 3D single particle tracking methods, highlighting specialized detection and excitation schemes which enable high-speed real-time tracking. Furthermore, the combination of these active feedback methods with simultaneous live-cell imaging is discussed. Finally, the successes in real-time 3D single molecule tracking (RT-3D-SMT) thus far and the roadmap going forward for this promising family of techniques are discussed.

Robust real-time 3D single-particle tracking using a dynamically moving laser spot

2017 ◽  
Vol 42 (12) ◽  
pp. 2390 ◽  
Author(s):  
Shangguo Hou ◽  
Xiaoqi Lang ◽  
Kevin Welsher
Keyword(s):  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Laser Spot

Visualizing the endocytic and exocytic processes of wheat germ agglutinin by quantum dot-based single-particle tracking

Biomaterials ◽  
2011 ◽  
Vol 32 (30) ◽  
pp. 7616-7624 ◽  
Author(s):  
Shu-Lin Liu ◽  
Zhi-Ling Zhang ◽  
En-Ze Sun ◽  
Jun Peng ◽  
Min Xie ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Particle Tracking ◽  
Single Particle ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Single Particle Tracking

Photoinduced mass transfer of azo polymers from micrometer to submillimeter studied by a real-time single particle strategy

Soft Matter ◽  
2020 ◽  
Vol 16 (42) ◽  
pp. 9746-9757
Author(s):  
Hao Huang ◽  
Chen Zhang ◽  
Jiaxing Lan ◽  
Zenan Wang ◽  
Xiaogong Wang
Keyword(s):  
Mass Transfer ◽  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Azo Polymers ◽  
Tracking Strategy

This article reports a real-time single particle tracking strategy to investigate the photoinduced mass transfer of azo polymers and the results.

scholarly journals Particle-by-Particle in Situ Characterization of the Protein Corona via Real-Time 3D Single Particle Tracking Microscopy

2021 ◽  
Author(s):  
Xiaochen Tan ◽  
Kevin Welsher
Keyword(s):  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Fluids ◽  
Protein Corona ◽  
Single Particle Tracking ◽  
Ex Situ ◽  
High Background ◽  
Particle Nature

<p>Nanoparticles (NPs) adsorb proteins when exposed to biological fluids, forming a dynamic protein corona that affects their fate in biological environments. A comprehensive understanding of the protein corona is lacking due to the inability of current techniques to precisely measure the full corona <i>in situ</i> at the single particle level. Herein, we introduce a 3D real-time single-particle tracking spectroscopy to "lock-on" to single freely-diffusing polystyrene NPs and probe their individual protein coronas. The diffusive motions of the tracked NPs enable quantification of the "hard corona" using mean-squared displacement analysis. Critically, this method's particle-by-particle nature enabled a lock-in-type frequency filtering approach to extract the full protein corona, despite the typically confounding effect of high background signal from unbound proteins. From these results, the dynamic <i>in situ </i>full protein corona is observed to contain double the number of proteins than are observed in the <i>ex situ</i> measured "hard" protein corona.</p><br>

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