scholarly journals Expression of Metazoan Annexins in Yeast Provides Protection Against Deleterious Effects of the Biofuel Isobutanol

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Carl E. Creutz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Heterologous Expression ◽  
Membrane Structure ◽  
Binding Proteins ◽  
Membrane Binding ◽  
Biofuel Production ◽  
Calcium Dependent ◽  
Complex Membrane ◽  
Industrial Strains

AbstractThe ability of microorganisms to produce biofuels by fermentation is adversely affected by the perturbing effects of the hydrophobic biofuel on plasma membrane structure. It is demonstrated here that heterologous expression of metazoan, calcium-dependent, membrane-binding proteins of the annexin class can reduce deleterious effects of isobutanol on Saccharomyces cerevisiae viability and complex membrane functions. Therefore, expression of annexins in industrial strains of yeast or bacteria may prove beneficial in biofuel production.

scholarly journals The PLUTO plastidial nucleobase transporter also transports the thiamin precursor hydroxymethylpyrimidine

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Guillaume A.W. Beaudoin ◽  
Timothy S. Johnson ◽  
Andrew D. Hanson
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Root Growth ◽  
Heterologous Expression ◽  
Transporter Gene ◽  
Bacterial Genomes ◽  
Thiamin Biosynthesis ◽  
Increased Sensitivity ◽  
Arabidopsis Homolog

In plants, the hydroxymethylpyrimidine (HMP) and thiazole precursors of thiamin are synthesized and coupled together to form thiamin in plastids. Mutants unable to form HMP can be rescued by exogenous HMP, implying the presence of HMP transporters in the plasma membrane and plastids. Analysis of bacterial genomes revealed a transporter gene that is chromosomally clustered with thiamin biosynthesis and salvage genes. Its closest Arabidopsis homolog, the plastidic nucleobase transporter (PLUTO), is co-expressed with several thiamin biosynthetic enzymes. Heterologous expression of PLUTO in Escherichia coli or Saccharomyces cerevisiae increased sensitivity to a toxic HMP analog, and disrupting PLUTO in an HMP-requiring Arabidopsis line reduced root growth at low HMP concentrations. These data implicate PLUTO in plastidial transport and salvage of HMP.

scholarly journals Two novel brain proteins, CaBP33 and CaBP37, are calcium-dependent phospholipid- and membrane-binding proteins

FEBS Letters ◽  
1990 ◽  
Vol 262 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Rosario Donato ◽  
Ileana Giambanco ◽  
Grazia Pula ◽  
Roberta Bianchi
Keyword(s):  
Binding Proteins ◽  
Membrane Binding ◽  
Brain Proteins ◽  
Calcium Dependent

scholarly journals Calcium-dependent Plasma Membrane Binding and Cell Lysis by Perforin Are Mediated through Its C2 Domain

2004 ◽  
Vol 280 (9) ◽  
pp. 8426-8434 ◽  
Author(s):  
Ilia Voskoboinik ◽  
Marie-Claude Thia ◽  
Jamie Fletcher ◽  
Annette Ciccone ◽  
Kylie Browne ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Binding ◽  
Cell Lysis ◽  
C2 Domain ◽  
Calcium Dependent ◽  
Plasma Membrane Binding
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