Upregulated TNF/Eiger signaling mediates stem cell recovery and tissue homeostasis during nutrient resupply in Drosophila testis

SummaryTissue homeostasis often requires a properly placed niche to support stem cells. The morphogenetic processes that position a niche are just being described. We recently showed that Drosophila testis pro niche cells, specified at disparate positions during early gonadogenesis, must assemble in one collective at the gonad anterior. Here, we identify Slit and FGF signals emanating from adjacent visceral mesoderm (Vm) that regulate assembly. In response to signaling, niche cells express islet, which we find is also required for positioning the niche. Without signaling, niche cells specified furthest from the anterior are unable to migrate, remaining dispersed. Function of the dispersed niche is severely disrupted, with pro-niche cells evading cell cycle quiescence, compromised in their ability to signal the incipient stem cell pool, and failing to orient stem cell divisions properly. Our work identifies both extrinsic signaling and intrinsic responses required for proper assembly and placement of the testis niche.

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Faculty Opinions recommendation of Identification of genes needed for regeneration, stem cell function, and tissue homeostasis by systematic gene perturbation in planaria.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1025766.304763 ◽

2005 ◽

Author(s):

Craig Crews

Keyword(s):

Stem Cell ◽

Cell Function ◽

Tissue Homeostasis ◽

Gene Perturbation

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Faculty Opinions recommendation of JAK-STAT signal inhibition regulates competition in the Drosophila testis stem cell niche.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1166226.793524568 ◽

2016 ◽

Author(s):

Hila Toledano

Keyword(s):

Stem Cell ◽

Stem Cell Niche ◽

Drosophila Testis ◽

Cell Niche

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Wnt6 regulates the homeostasis of the stem cell niche via Rac1-and Cdc42-mediated noncanonical Wnt signalling pathways in Drosophila testis

Experimental Cell Research ◽

10.1016/j.yexcr.2021.112511 ◽

2021 ◽

Vol 402 (1) ◽

pp. 112511

Author(s):

Min Wang ◽

Xiaojin Luan ◽

Yidan Yan ◽

Qianwen Zheng ◽

Wanyin Chen ◽

...

Keyword(s):

Stem Cell ◽

Stem Cell Niche ◽

Wnt Signalling ◽

Signalling Pathways ◽

Drosophila Testis ◽

Cell Niche

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Abstract 160: Pim-1 Preserves Cardiac Stem Cell Proliferation with Age

Circulation Research ◽

10.1161/res.111.suppl_1.a160 ◽

2012 ◽

Vol 111 (suppl_1) ◽

Author(s):

Michael McGregor ◽

Shabana Din ◽

Natalie Gude ◽

Mark A Sussman

Keyword(s):

Cell Proliferation ◽

Stem Cell ◽

Time Course ◽

Protein A ◽

Cell Types ◽

Tissue Homeostasis ◽

Dominant Negative ◽

Repair Capacity ◽

Tissue Samples ◽

The Impact

Rationale Cardiac stem cells (CSC) regulate cardiomyogenesis and support regenerative processes in the heart, but aging adversely affects stem cell repair capacity. Aging is a primary cause of impaired cardiac function characterized by accumulation of senescent cells. CSC senescence is associated with permanent growth arrest that decreases survival signaling and cellular replacement, inevitably diminishing the capacity of the heart to maintain tissue homeostasis. Therefore, promoting CSC growth may improve cardiac performance with age. Pim-1 kinase exhibits protective and proliferative effects in the myocardium but the role of Pim-1 in cardiac aging has not been thoroughly studied. Objective Demonstrate that Pim-1 promotes stem cell growth in the aged myocardium correlating with increased expression of centromere protein A (CENP-A), a kinetochore-associated protein known to support cell proliferation in numerous species and cell types. Methods & Results CENP-A expression levels were evaluated from murine myocardial tissue samples ranging in age from 11 days post coitum to 4 months of age with analysis by immunoblot as well as quantitative PCR. CENP-A expression was colocalized with c-kit as a marker of CSC by immunohistochemical labeling, revealing a decline in CENP-A expression over the time course of postnatal myocardial maturation. The impact of Pim-1 upon CENP-A level was assessed by comparative analysis of non-transgenic mice versus genetically modified transgenic mouse lines expressing either Pim-1 (wild type) or a dominant negative functionally dead Pim-1 mutant. Pim-1 overexpression increases persistence of CENP-A in CSCs with age, as well as the prevalence of cycling CSCs as marked by phosph-H3 expression, while the functionally dead mutant accelerates CENP-A diminution and decreases CSC proliferation. Conclusion CENP-A decline in c-kit positive cells with age provides intriguing evidence of a potential mechanism for the diminished capacity of CSCs to maintain tissue homeostasis. Pim-1 mitigates CENP-A diminution, demonstrating the promising potential of Pim-1 to promote cardiac growth and repair with age.

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TotiCyte, a Paradigm Shift in Stem Cell Isolation and Storage from Umbilical Cord Blood

Stem Cells Research Development & Therapy ◽

10.24966/srdt-2060/100073 ◽

2021 ◽

Vol 7 (3) ◽

pp. 1-7

Author(s):

Lesley-Ann Martin ◽

Keyword(s):

Stem Cell ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Paradigm Shift ◽

Cell Isolation ◽

Volume Reduction ◽

Cell Recovery ◽

Stem Cell Isolation ◽

And Storage

We provide evidence to support the use of TotiCyte as a novel volume reduction technology capable of significantly improving CD34+ stem cell recovery

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Lin28 is a critical factor in the function and aging of Drosophila testis stem cell niche

Aging ◽

10.18632/aging.101765 ◽

2019 ◽

Vol 11 (3) ◽

pp. 855-873

Author(s):

Perinthottathil Sreejith ◽

Wijeong Jang ◽

Van To ◽

Yong Hun Jo ◽

Benoit Biteau ◽

...

Keyword(s):

Stem Cell ◽

Stem Cell Niche ◽

Critical Factor ◽

Drosophila Testis ◽

Cell Niche

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Thyroid-Specific Transcription Factors and Their Roles in Thyroid Cancer

Journal of Thyroid Research ◽

10.4061/2011/710213 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8 ◽

Cited By ~ 22

Author(s):

Shioko Kimura

Keyword(s):

Stem Cell ◽

Transcription Factors ◽

Thyroid Cancer ◽

Cancer Biology ◽

Tissue Homeostasis ◽

Specific Gene ◽

Paired Domain ◽

Hormone Synthesis ◽

Forkhead Domain ◽

Thyroid Development

Homeodomain, forkhead domain, and paired domain-containing transcription factors play a major role in development, tissue-specific gene expression, and tissue homeostasis in organs where they are expressed. Recently, their roles in stem cell and cancer biology are emerging. In the thyroid, NKX2-1, FOXE1, and PAX8 transcription factors are responsible for thyroid organogenesis and expression of thyroid-specific genes critical for thyroid hormone synthesis. In contrast to their known roles in gene regulation, thyroid development and homeostasis, their involvement in stem cell, and/or cancer biology are still elusive. In order to further understand the nature of thyroid cancer, it is critical to determine their roles in thyroid cancer.

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