scholarly journals Proteomics provides insights into the inhibition of Chinese hamster V79 cell proliferation in the deep underground environment

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jifeng Liu ◽  
Tengfei Ma ◽  
Mingzhong Gao ◽  
Yilin Liu ◽  
Jun Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dose Rate ◽  
Background Radiation ◽  
Chinese Hamster ◽  
Biological Study ◽  
V79 Cells ◽  
Deep Underground ◽  
Γ Ray ◽  
V79 Cell ◽  
Cells Cultured

Abstract As resources in the shallow depths of the earth exhausted, people will spend extended periods of time in the deep underground space. However, little is known about the deep underground environment affecting the health of organisms. Hence, we established both deep underground laboratory (DUGL) and above ground laboratory (AGL) to investigate the effect of environmental factors on organisms. Six environmental parameters were monitored in the DUGL and AGL. Growth curves were recorded and tandem mass tag (TMT) proteomics analysis were performed to explore the proliferative ability and differentially abundant proteins (DAPs) in V79 cells (a cell line widely used in biological study in DUGLs) cultured in the DUGL and AGL. Parallel Reaction Monitoring was conducted to verify the TMT results. γ ray dose rate showed the most detectable difference between the two laboratories, whereby γ ray dose rate was significantly lower in the DUGL compared to the AGL. V79 cell proliferation was slower in the DUGL. Quantitative proteomics detected 980 DAPs (absolute fold change ≥ 1.2, p < 0.05) between V79 cells cultured in the DUGL and AGL. Of these, 576 proteins were up-regulated and 404 proteins were down-regulated in V79 cells cultured in the DUGL. KEGG pathway analysis revealed that seven pathways (e.g. ribosome, RNA transport and oxidative phosphorylation) were significantly enriched. These data suggest that proliferation of V79 cells was inhibited in the DUGL, likely because cells were exposed to reduced background radiation. The apparent changes in the proteome profile may have induced cellular changes that delayed proliferation but enhanced survival, rendering V79 cells adaptable to the changing environment.

scholarly journals Proteomics provides insights into the inhibition of Chinese hamster V79 cell proliferation in the deep underground environment

2020 ◽  
Author(s):  
Shixi Liu ◽  
Jifeng Liu ◽  
Tengfei Ma ◽  
Mingzhong Gao ◽  
Yilin Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Chinese Hamster ◽  
Enrichment Analysis ◽  
Cosmic Ray ◽  
Rna Transport ◽  
V79 Cells ◽  
Term Enrichment Analysis ◽  
Term Enrichment ◽  
Deep Underground ◽  
Cells Cultured

Abstract Background: To characterize the environment of deep underground laboratory (DUGL) with a rock cover of 1470m and observe the effect of the DUGL environment on the growth and metabolism of Chinese hamster V79 cells. Results: Six environmental parameters in the DUGL and an above ground laboratory (AGL; control) were monitored. Compared to the AGL, O2 concentration was not significantly different, total γ ray dose rate was significantly lower (p=0.005), and relative humidity (p<0.001), air pressure (p<0.001), and concentration of CO2 and radon gas (p<0.001) were significantly higher in the DUGL. The growth curves of cultured V79 cells showed cell proliferation was slower in the DUGL. Tandem mass tag (TMT) proteomics analysis was performed to identify differentially abundant proteins (DAPs) in V79 cells cultured in the DUGL and AGL. Parallel Reaction Monitoring (PRM) was conducted to verify TMT results. TMT detected 980 DAPs, defined as proteins with a ≥1.2- absolute fold change in relative abundance (p <0.05) between V79 cells cultured in the DUGL and AGL. Of these, 576 proteins were up-regulated and 404 proteins were down-regulated in V79 cells cultured in the DUGL. GO term enrichment analysis of up-regulated proteins revealed enrichment of proteins involved in translation, ribosome, proton-transporting ATP synthase activity, oxygen binding, and oxygen transporter activity et al. GO term enrichment analysis of down-regulated proteins demonstrated enrichment of proteins involved in the endoplasmic reticulum lumen and respiratory chain. KEGG pathway analysis revealed that ribosome (p<0.001), base excision repair (p<0.001), RNA transport (p=0.009), Huntington's disease (p=0.023), and oxidative phosphorylation (OXPPL) (p=0.035) pathways were significantly enriched. Conclusion: Proliferation of V79 cells was inhibited in the DUGL, likely because cells were exposed to reduced cosmic ray muons flux. There were apparent changes in the proteome profile of the V79 cells cultured in the DUGL, which affected proteins related to the ribosome, RNA transport, translation, energy metabolism, and DNA repair. These proteins may have induced cellular changes that delayed proliferation but enhanced survival, making the V79 cells adaptable to the changing environment. Our findings provide insight into the cellular stress response that is triggered in the absence of normal levels of radiation.

scholarly journals Whole Transcriptome Analysis Revealed a Stress Response to Deep Underground Environment Conditions in Chinese Hamster V79 Lung Fibroblast Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Liju Duan ◽  
Hongying Jiang ◽  
Jifeng Liu ◽  
Yilin Liu ◽  
Tengfei Ma ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Background Radiation ◽  
Lung Fibroblast ◽  
Fibroblast Cells ◽  
V79 Cells ◽  
Significant Difference ◽  
Deep Underground ◽  
Whole Transcriptome Analysis ◽  
Whole Transcriptome ◽  
Cells Cultured

Background: Prior studies have shown that the proliferation of V79 lung fibroblast cells could be inhibited by low background radiation (LBR) in deep underground laboratory (DUGL). In the current study, we revealed further molecular changes by performing whole transcriptome analysis on the expression profiles of long non-coding RNA (lncRNA), messenger RNA (mRNA), circular RNA (circRNA) and microRNA (miRNA) in V79 cells cultured for two days in a DUGL.Methods: Whole transcriptome analysis including lncRNA, mRNAs, circ RNA and miRNA was performed in V79 cells cultured for two days in DUGL and above ground laboratory (AGL), respectively. The differentially expressed (DE) lncRNA, mRNA, circRNA, and miRNA in V79 cells were identified by the comparison between DUGL and AGL groups. Quantitative real-time polymerase chain reaction(qRT-PCR)was conducted to verify the selected RNA sequencings. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was analyzed for the DE mRNAs which enabled to predict target genes of lncRNA and host genes of circRNA.Results: With |log2(Fold-change)| ≥ 1.0 and p &lt; 0.05, a total of 1257 mRNAs (353 mRNAs up-regulated, 904 mRNAs down-regulated), 866 lncRNAs (145 lncRNAs up-regulated, 721 lncRNAs down-regulated), and 474 circRNAs (247 circRNAs up-regulated, 227 circRNAs down-regulated) were significantly altered between the two groups. There was no significant difference in miRNA between the two groups. The altered RNA profiles were mainly discovered in lncRNAs, mRNAs and circRNAs. DE RNAs were involved in many pathways including ECM-RI, PI3K-Akt signaling, RNA transport and the cell cycle under the LBR stress of the deep underground environment.Conclusion: Taken together, these results suggest that the LBR in the DUGL could induce transcriptional repression, thus reducing metabolic process and reprogramming the overall gene expression profile in V79 cells.

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Ivan Pavicic ◽  
Ivancica Trosic
Keyword(s):  
Cell Proliferation ◽  
Electromagnetic Field ◽  
Continuous Wave ◽  
Biological Effects ◽  
Chinese Hamster ◽  
Established Cell Line ◽  
V79 Cells ◽  
Trypan Blue Exclusion ◽  
Cell Counts ◽  
Established Cell

AbstractConsidering often contradictory data on biological effects of mobile phones frequencies on established cell culture lines, our study aimed at evaluating the influence of 864 MHz electromagnetic field on proliferation, colony forming ability and viability of Chinese hamster lung cells continuous line V79. Prior to exposure for 1, 2 and 3 hours in transversal electromagnetic mode cell (TEM-cell) equipped by Philips PM 5508 signal generator cell samples were sub-cultivated for one day. Cell samples were exposed to 864 MHz continuous wave at an average specific absorption rate (SAR) of 0.08 W/kg. To determine cell growth, V79 cells were plated in concentration of 1 × 104 cells per milliliter of nutrient medium RPMI 1640, and raised in a humified atmosphere at 37°C in 5% CO2. Cell proliferation was determined by cell counts for each hour of exposure on post-exposure day 1, 2, 3, 4 and 5. To identify colony-forming ability, cells were cultivated in concentration of 40 cells/mL of RPMI 1640 and incubated according to the deliberated experimental protocol. Colony forming ability for each hour of exposure was defined by colony counts on experimental day 7. Trypan blue exclusion test was used to determine viability of cells. In comparison to sham-exposed cells, growth curve of irradiated cell samples showed significant decrease (p < 0.05) after 2 and 3 hours of exposure on experimental day 3, respectively. Both, the colony forming ability and viability of irradiated cells did not significantly differ from exposed “mock” condition. Under strictly controlled laboratory conditions, applied radiofrequency microwaves (RF/MW) irradiation significantly affected cell proliferation kinetics but not viability or ability of V79 cells to form colonies. Sophisticated mechanism of action is intending to be elucidated in the further research which will include insight into the RF/MW related event at the subcellular level.

Cytotoxic and Genotoxic Effects of Sodium Fluoride on Mammalian Cells at Neutral or Acid pH: A Summary of Published Work

1997 ◽  
Vol 25 (3) ◽  
pp. 341-342
Author(s):  
Darina Slameñová ◽  
Alena Gábelová ◽  
Katarina Ruppová ◽  
Ladislave Wsólová
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Sodium Fluoride ◽  
Mammalian Cells ◽  
Chinese Hamster ◽  
Short Review ◽  
Genotoxic Effects ◽  
Acid Ph ◽  
Mutagenic Effects ◽  
V79 Cell

This paper forms a short review of some of our previously published work. We assessed the cytotoxic and genotoxic effects of sodium fluoride (NaF) on the Chinese hamster V79 cell line and the human EUE cell line at neutral and acid pH (as found in the stomach). The effects of NaF on cell proliferation, DNA synthesis, protein synthesis and mutagenesis were assessed. The results found showed no mutagenic effects after exposure to NaF, despite some cytogenic effects, suggesting that NaF probably does not represent any serious mutagenic or carcinogenic threat to humans.

scholarly journals Studies on the differential inhibition of glutathione conjugate formation of (+)-anti-benzo[a]pyrene 7,8-dihydrodiol 9,10-epoxide and 1-chloro-2,4-dinitrobenzene in V79 Chinese hamster cells

2000 ◽  
Vol 349 (3) ◽  
pp. 693-696 ◽  
Author(s):  
Kathrin SUNDBERG ◽  
Bengt JERNSTRÖM ◽  
Stellan SWEDMARK
Keyword(s):  
Molecular Mass ◽  
Glutathione Transferase ◽  
Chinese Hamster ◽  
Cell Extract ◽  
Differential Inhibition ◽  
V79 Cells ◽  
Chinese Hamster Cells ◽  
Carcinogenic Compound ◽  
V79 Cell ◽  
Mcf 7

V79 Chinese hamster cells have previously been shown to lack the capacity to detoxify the mutagenic and carcinogenic compound (+)-anti-benzo[a]pyrene 7,8-dihydrodiol 9,10-epoxide [(+)-anti-BPDE] by Pi class glutathione transferase (GSTPi)-catalysed conjugation with GSH, although these cells contain such an enzyme [Romert, Dock, Jenssen and Jernström (1989) Carcinogenesis 10, 1701–1707; Swedmark, Romert, Morgenstern and Jenssen (1992) Carcinogenesis 13, 1719–1723; Swedmark and Jenssen (1994) Gene 139, 251–256]. Previous findings also indicate that these results do not depend on an inactive GSTPi enzyme, since V79 cells conjugate 1-chloro-2,4-dinitrobenzene (CDNB) with GSH, but more likely on (a) factor(s) that inhibit(s) V79 GSTPi selectively [Swedmark, Jernström and Jenssen (1996) Biochem. J. 318, 533–538]. The present study demonstrates that both human and V79 recombinant GSTPi enzymes are inhibited with respect to conjugating (+)-anti-BPDE, but not CDNB, after pre-incubation with V79-cell extract, but not with MCF-7-cell extract. In addition, it was found that the inhibition is dependent on the amount of cell extract present and that the factor(s) is heat-resistant and has a molecular mass of less than 10 kDa, suggesting that the factor(s) is (are) non-proteinaceous in nature.

HUMAN EXPOSURE TO BACKGROUND RADIATION IN ORTUM, KENYA

2019 ◽  
Vol 188 (1) ◽  
pp. 98-108
Author(s):  
F O Wanjala ◽  
N O Hashim ◽  
D Otwoma ◽  
C Nyambura ◽  
J Kebwaro ◽  
...  
Keyword(s):  
Dose Rate ◽  
Human Exposure ◽  
Activity Concentration ◽  
Background Radiation ◽  
Absorbed Dose ◽  
Threshold Values ◽  
Absorbed Dose Rate ◽  
The World ◽  
Average Activity ◽  
Terrestrial Radionuclides

Abstract The activity concentration of radionuclides 238U, 232Th and 40K in soil and the absorbed dose rate (ADRA) at 1 m above the ground in Ortum was determined. The activity concentration in soils ranged from 33 to 85, 20 to 67 and 148–1019 Bq kg–1, respectively with an average of 40 ± 1.43, 56 ± 1.46 and 425 ± 19.24 Bq kg–1, respectively. The activity concentration of 232Th and 238U was found to reduce with increasing depth while that of 40K increased with increasing depth. The average activity concentration in soil was higher than the world average values. The average ADRA in air at 1 m above the ground was found to be 112 ± 29.6 nGy h–1. The soil and rocks in Ortum are recommended for use because the activity concentration of the terrestrial radionuclides is lower than the recommended threshold values.

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