scholarly journals In-vitro assessment of appropriate hydrophilic scaffolds by co-electrospinning of poly(1,4 cyclohexane isosorbide terephthalate)/polyvinyl alcohol

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Abdul Salam ◽  
Muhammad Qamar Khan ◽  
Tufail Hassan ◽  
Nafees Hassan ◽  
Ahsan Nazir ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Dimensional Stability ◽  
Biocompatible Polymers ◽  
Dual Nature ◽  
Hydrophilic Behavior ◽  
In Vitro Assessment ◽  
Culture Growth ◽  
Bio Compatibility ◽  
Cell Culture Growth

AbstractTextile-based Scaffolds preparation has the attractive features to fulfill the stated and implied needs of the consumer but there are still challenges of stability, elongation, appreciable bio-compatibility, and stated hydrophilic behavior. To overcome these challenges, the authors tried to fabricate a scaffold by blending of two highly biocompatible polymers; polyvinyl alcohol and poly(1,4 cyclohexane isosorbide terephthalate) through co-electrospinning. The resultant scaffold by the stated innovative approach evaluated from different characterizations such as dimensional stability/morphology was evaluated by scanning electron microscopy, chemical interactions by that Fourier transmission infrared spectra, wetting behavior was analyzed by a static angle with a contact angle meter from drop method, elongation was examined by tensile strength tester and in-vitro assessment was done by MTT analysis. Based on verified results, it was concluded that PVA/PICT scaffold has a potential for dual nature of hydrophilicity & hydrophobicity and appreciable cell culture growth, stated dimensional stability and suitable elongation as per requirements of the nature of scaffold.

EFFECT OF TRIGONA HONEY ON Escherichia Coli CELL CULTURE GROWTH: IN VITRO STUDY

2019 ◽  
Vol 5 (2) ◽  
pp. 10
Author(s):  
Mohammad Kafaween ◽  
Abu Hilmi ◽  
Rao Khan ◽  
Mabrouka BOUACHA ◽  
Malik Amonov
Keyword(s):  
Escherichia Coli ◽  
Cell Culture ◽  
In Vitro Study ◽  
Vitro Study ◽  
Coli Cell ◽  
Culture Growth ◽  
Cell Culture Growth

Melanoma Cell Lines Role in Obtaining New Drug Candidates for Combating the Malignant Pathology of the Cutaneous Organ

2019 ◽  
Vol 70 (3) ◽  
pp. 943-945
Author(s):  
Zsolt Gyori ◽  
Monica Susan ◽  
Razvan Susan ◽  
Andrada Iftode ◽  
Cristina Trandafirescu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Point Of View ◽  
In Vitro Studies ◽  
Drug Candidates ◽  
Starting Point ◽  
In Vivo Animal Models ◽  
Culture Growth ◽  
Cell Culture Growth

As prophylactic and therapeutic approaches for melanoma, of great interest and importance are the in vitro studies using cell lines to elucidate several tumoral phenomena. Therefore, the similarities and differences between the different tumor cells must be known and understood in order to obtain a more accurate correlation with processes that occur in vivo. In this study, six cell lines of melanoma, both of mouse and human origin were analyzed from the point of view of cell culture growth, morphology and use in the research of new therapies. In brief, the current paper exhibits a comparison of melanoma cells which can be utilized as a starting point for further in vitro studies and in vivo animal models.

In vitro assessment of Labisia pumila and its constituents for interactions with CYPs, P-GP and PXR

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
VK Manda ◽  
OR Dale ◽  
C Awortwe ◽  
Z Ali ◽  
IA Khan ◽  
...  
Keyword(s):  
In Vitro Assessment ◽  
Labisia Pumila

ANALYSIS OF THE BIPHASIC ACTION OF GROWTH HORMONE IN VITRO ON THE RAT DIAPHRAGM

1968 ◽  
Vol 57 (3_Suppl) ◽  
pp. S19-S35 ◽  
Author(s):  
Å. Hjalmarson
Keyword(s):  
Growth Hormone ◽  
Actinomycin D ◽  
Accumulation Rate ◽  
Inhibitory Effect ◽  
Bovine Growth Hormone ◽  
Rat Diaphragm ◽  
Dual Nature ◽  
Hypophysectomized Rats ◽  
D 20

ABSTRACT In vitro addition of bovine growth hormone (GH) to intact hemidiaphragms from hypophysectomized rats has previously been found to produce both an early stimulatory effect lasting for 2—3 hours and a subsequent late inhibitory effect during which the muscle is insensitive to further addition of GH (Hjalmarson 1968). These effects on the accumulation rate of α-aminoisobutyric acid (AIB) and D-xylose have been further studied. In presence of actinomycin D (20 μg/ml) or puromycin (100 μg/ml) the duration of the stimulatory effect of GH (25 μg/ml) was prolonged to last for at least 4—5 hours and the late inhibitory effect was prevented. Similar results were obtained when glucose-free incubation medium was used. Preincubation of the diaphragm at different glucose concentrations (0—5 mg/ml) for 3 hours did not change the GH sensitivity. Addition of insulin at start of incubation could not prevent GH from inducing its late inhibitory effect, while dexamethasone seemed to potentiate this effect of GH. Furthermore, adrenaline was found to decrease the uptake of AIB-14C and D-xylose-14C in the diaphragm, but not to change the sensitivity of the muscle to GH. Preincubation of the diaphragm for 3 hours with puromycin in a concentration of 200 μg/ml markedly decreased the subsequent basal uptake of both AIB-14C and D-xylose-14C, in the presence of puromycin, and abolished the stimulatory effect of GH on the accumulation of AIB-14C. However, the effect of GH on the accumulation of D-xylose-14C was unchanged. The present observations are discussed and evaluated in relation to various mechanisms of GH action proposed to explain the dual nature of the hormone.

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