scholarly journals Artifact-free holographic light shaping through moving acousto-optic holograms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dorian Treptow ◽  
Raúl Bola ◽  
Estela Martín-Badosa ◽  
Mario Montes-Usategui
Keyword(s):  
Optical Power ◽  
Spatial Light Modulators ◽  
Structured Illumination ◽  
Light Modulation ◽  
Laser Material Processing ◽  
Structured Illumination Microscopy ◽  
Light Modulators ◽  
High Modulation ◽  
Optical Output ◽  
The Many

AbstractHolographic light modulation is the most efficient method to shape laser light into well-defined patterns and is therefore the means of choice for many intensity demanding applications. During the last two decades, spatial light modulators based on liquid crystals prevailed among several technologies and became the standard tool to shape light holographically. But in the near future, this status might be challenged by acousto-optic deflectors. These devices are well known for their excelling modulation rates and high optical power resilience. But only few scattered precedents exist that demonstrate their holographic capabilities, despite the many interesting properties that they provide. We implemented a holographic acousto-optic light modulation (HALM) system, that is based on displaying holograms on acousto-optic deflectors. We found that this system can eliminate the ubiquitous coherent artifacts that arise in holography through the inherent motion of acousto-optic holograms. That distinguishes our approach from any other holographic modulation technique and allows to reconstruct intensity patterns of the highest fidelity. A mathematical description of this effect is presented and experimentally confirmed by reconstructing images holographically with unprecedented quality. Our results suggest that HALM promotes acousto-optic deflectors from highly specialized devices to full-fledged spatial light modulators, that can compete in a multitude of applications with LC-SLMs. Especially applications that require large optical output powers, high modulation speeds or accurate gray-scale intensity patterns will profit from this technology. We foresee that HALM may play a major role in future laser projectors and displays, structured illumination microscopy, laser material processing and optical trapping.

scholarly journals High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Nanophotonics ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 143-148
Author(s):  
Adrien Descloux ◽  
Marcel Müller ◽  
Vytautas Navikas ◽  
Andreas Markwirth ◽  
Robin van den Eynde ◽  
...  
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Super Resolution ◽  
Optical Element ◽  
Spatial Light Modulators ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Image Stack ◽  
Light Modulators ◽  
3D Information

AbstractSuper-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work, we present a multiplane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multiplane image splitting prism combined with high-speed SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally highly resolved 3D image stack. We demonstrate the performance of multiplane SIM by applying this instrument to imaging the dynamics of mitochondria in living COS-7 cells.

scholarly journals High speed multi-plane super-resolution structured illumination microscopy of living cells using an image-splitting prism

2019 ◽  
Author(s):  
Adrien Descloux ◽  
Marcel Müller ◽  
Vytautas Navikas ◽  
Andreas Markwirth ◽  
Robin Van den Eynde ◽  
...  
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Super Resolution ◽  
Optical Element ◽  
Spatial Light Modulators ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Image Stack ◽  
Light Modulators ◽  
3D Information

Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work we present a multi-plane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multi-plane image splitting prism combined with high-speed SR-SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally super-resolved three-dimensional image stack. We demonstrate the performance of multi-plane SR-SIM by applying this instrument to the dynamics of live mitochondrial network.

scholarly journals Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

2020 ◽  
Author(s):  
Mario Lachetta ◽  
Hauke Sandmeyer ◽  
Alice Sandmeyer ◽  
Jan Schulte am Esch ◽  
Thomas Huser ◽  
...  
Keyword(s):  
High Speed ◽  
Consumer Electronics ◽  
Light Sources ◽  
Structured Illumination ◽  
Light Modulation ◽  
Coherent Light ◽  
Structured Illumination Microscopy ◽  
Excitation Light ◽  
Coherent Excitation ◽  
Digital Micromirror Devices

SummaryDigital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected of a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video projectors, and scientific applications such as microscopy.High-end fluorescence microscopy systems typically employ laser light sources, which by their nature provide coherent excitation light. In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. The universal combination of DMDs and coherent light sources, especially when working with multiple different wavelengths, is unfortunately not straight forward. The substructure of the tilted micromirror array gives rise to a blazed grating, which has to be understood and which must be taken into account when designing a DMD-based illumination system.Here, we present a set of simulation frameworks that explore the use of DMDs in conjunction with coherent light sources, motivated by their application in SIM, but which are generalizable to other light patterning applications. This framework provides all the tools to explore and compute DMD-based diffraction effects and to simulate possible system alignment configurations computationally, which simplifies the system design process and provides guidance for setting up DMD-based microscopes.

scholarly journals Microscope-Cockpit: Python-based bespoke microscopy for bio-medical science

2021 ◽  
Vol 6 ◽  
pp. 76
Author(s):  
Mick A. Phillips ◽  
David Miguel Susano Pinto ◽  
Nicholas Hall ◽  
Julio Mateos-Langerak ◽  
Richard M. Parton ◽  
...  
Keyword(s):  
Open Source ◽  
Adaptive Optics ◽  
High Performance ◽  
Medical Science ◽  
Machine Learning Algorithms ◽  
Spatial Light Modulators ◽  
Structured Illumination ◽  
Light Modulators ◽  
Wide Range ◽  
User Friendly

We have developed “Microscope-Cockpit” (Cockpit), a highly adaptable open source user-friendly Python-based Graphical User Interface (GUI) environment for precision control of both simple and elaborate bespoke microscope systems. The user environment allows next-generation near instantaneous navigation of the entire slide landscape for efficient selection of specimens of interest and automated acquisition without the use of eyepieces. Cockpit uses “Python-Microscope” (Microscope) for high-performance coordinated control of a wide range of hardware devices using open source software. Microscope also controls complex hardware devices such as deformable mirrors for aberration correction and spatial light modulators for structured illumination via abstracted device models. We demonstrate the advantages of the Cockpit platform using several bespoke microscopes, including a simple widefield system and a complex system with adaptive optics and structured illumination. A key strength of Cockpit is its use of Python, which means that any microscope built with Cockpit is ready for future customisation by simply adding new libraries, for example machine learning algorithms to enable automated microscopy decision making while imaging.

scholarly journals UV Durable LCOS for Laser Processing

Crystals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1047
Author(s):  
Yasuki Sakurai ◽  
Masashi Nishitateno ◽  
Masahiro Ito ◽  
Kohki Takatoh
Keyword(s):  
Liquid Crystal ◽  
Laser Processing ◽  
Optical Element ◽  
Spatial Light Modulators ◽  
Light Sources ◽  
Laser Material Processing ◽  
Light Modulator ◽  
Light Modulators ◽  
Liquid Crystal On Silicon ◽  
Wavelength Light

Liquid-Crystal-On-Silicon (LCOS) Spatial Light Modulator (SLM) is widely used as a programmable adaptive optical element in many laser processing applications with various wavelength light sources. We report UV durable liquid-crystal-on-silicon spatial light modulators for one-shot laser material processing. Newly developed LCOS consists of UV transparent materials and shows a lifetime 480 times longer than the conventional one in 9.7 W/cm2 illumination at 355 nm. We investigated the durability of polymerization inhibitor mixed liquid crystal in order to extend its lifetime.

scholarly journals Graphene-Based Spatial Light Modulator Using Metal Hot Spots

Materials ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3082 ◽  
Author(s):  
Zhanshan Sun ◽  
Yuejun Zheng ◽  
Yunqi Fu
Keyword(s):  
Electric Field ◽  
Hot Spots ◽  
Near Infrared ◽  
Modulation Depth ◽  
Field Enhancement ◽  
Spatial Light Modulators ◽  
Light Modulator ◽  
Light Modulators ◽  
High Modulation ◽  
Doped Graphene

Here, we report a graphene-based electric field enhancement structure achieved by several adjacent metal nanoribbons which form the hot spots of the electric field and thus promote the absorption of the single layered graphene below the hot spots. Based on the tunability of the graphene’s Fermi level, the absorption rate can be modulated from near 100% to 35% under low electrostatic gating, leading to a 20 dB modulation depth of reflectance. Compared with the existing near infrared spatial light modulators such as optical cavities integrated with graphene and other structures utilizing patterned or highly doped graphene, our design has the advantages of strong optical field enhancement, low power dissipation and high modulation depth. The proposed electro-optic modulator has a promising potential for developing optical communication and exploiting big data interaction systems.

scholarly journals Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale

2020 ◽  
Author(s):  
Hadrien Robert ◽  
Łukasz Bujak ◽  
Kristýna Holanová ◽  
Milan Vala ◽  
Piliarik Marek
Keyword(s):  
Super Resolution ◽  
Light Polarization ◽  
Spatial Light Modulators ◽  
Phase Imaging ◽  
Light Modulation ◽  
Quantitative Phase Imaging ◽  
Light Modulator ◽  
Quantitative Phase ◽  
Light Modulators ◽  
Spatial Light Modulation

Abstract Spatial light modulators have become an essential tool for advanced microscopy enabling breakthroughs in 3D, phase, or super-resolution imaging. However, continuous spatial-light modulation without diffraction artifacts, polarization dependence, and able to capture sub-ms microscopic motion is challenging. Here we present a photothermal spatial light modulator (PT-SLM) enabling the fast wavefront shaping free of diffraction artifacts, having a high transmissivity and modulation efficiency independent of light polarization. It is based on the microscopic heating of a thin layer of thermo-optic material confined between the photothermal heat-source and a transparent heatsink. We achieve a phase-shift > π with a response time as short as 70 µs with a theoretical limit in the sub-µs range. The combination of the PT-SLM with an interferometric scattering microscope (iSCAT) allowed us to perform quantitative phase imaging of sub-diffractional scatterers and decipher the 3D nanoscopic displacement of microtubules matching closely with control data from atomic force microscopy.

scholarly journals A new spin on single-pixel imaging: spatial light modulation at megahertz switch rates via cyclic Hadamard masks

2021 ◽  
Author(s):  
Amir Rosenthal ◽  
Evgeny Hahamovich ◽  
Sagi Monin ◽  
Yoav Hazan
Keyword(s):  
Dynamic Imaging ◽  
Spatial Modulation ◽  
Spatial Light Modulators ◽  
Wide Field ◽  
Light Modulation ◽  
C Elegans ◽  
Light Modulators ◽  
Single Detector ◽  
Single Pixel

Abstract Optical imaging is commonly performed with either a camera and wide-field illumination or with a single detector and a collimated beam that scans the imaged object. Unfortunately, sources that can be collimated and cameras do not exist at all wavelengths and may not always achieve the specifications required for a given application. Single-pixel imaging (SPI) offers an alternative that requires a single detector and may be performed with wide-field illumination, potentially enabling imaging applications in which both the detection and illumination technologies are immature. However, SPI currently struggles with low imaging rates owing to its reliance on configurable spatial light modulators, whose rates do not generally exceed 22 kHz. In this work, we develop an approach towards rapid SPI which relies on cyclic patterns coded onto a spinning mask and demonstrate it for in vivo imaging of C. elegans worms. Spatial modulation rates of up to 2.4 MHz and imaging rates of up to 72 fps are reported, creating new opportunities for using the SPI paradigm in dynamic imaging scenarios.

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