scholarly journals Relationship between delayed luminescence emission and mitochondrial status in Saccharomyces cerevisiae

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Miao Tian ◽  
Qing Li ◽  
Yang Liu ◽  
Peng Zheng ◽  
Danyu Li ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Detection System ◽  
Mitochondrial Morphology ◽  
Delayed Luminescence ◽  
Mtdna Copy Number ◽  
Mitochondria Membrane Potential ◽  
Cell Vitality ◽  
The Relationship ◽  
Glucose Supply ◽  
Cells Cultured

AbstractDelayed luminescence (DL) is gradually used in various detection of biological systems as a rapid detection technique, however, its biological mechanism was still not clear. In this study, a new model of DL detection system for liquid biological samples is established to investigate the DL emission of Saccharomyces cerevisiae cells cultured in different glucose concentrations. We analyzed the relationship between the DL emission and cell growth, cell vitality, mitochondrial morphology, mitochondrial DNA (mtDNA) copy number, adenosine triphosphate (ATP), oxygen consumption rate (OCR), as well as mitochondria membrane potential (MMP) in S. cerevisiae cells cultured with 0.01, 0.05, 0.15, 3, 10 and 20 g/L glucose respectively. It was found that the DL emission had strong correlation with mitochondrial morphology, OCR, and MMP. The results suggested that DL is an indicator of mitochondria status under different glucose supply conditions, and may be an effective method to detect mitochondrial metabolism related disorders.

scholarly journals GIT1, a Gene Encoding a Novel Transporter for Glycerophosphoinositol in Saccharomyces cerevisiae

Genetics ◽  
1998 ◽  
Vol 149 (4) ◽  
pp. 1707-1715 ◽  
Author(s):  
J L Patton-Vogt ◽  
S A Henry
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Regulatory Gene ◽  
Sugar Transport ◽  
Open Reading Frame ◽  
Gene Encoding ◽  
Reading Frame ◽  
Membrane Spanning ◽  
Membrane Spanning Domains ◽  
Uptake And Metabolism ◽  
Cells Cultured

Abstract Phosphatidylinositol catabolism in Saccharomyces cerevisiae cells cultured in media containing inositol results in the release of glycerophosphoinositol (GroPIns) into the medium. As the extracellular concentration of inositol decreases with growth, the released GroPIns is transported back into the cell. Exploiting the ability of the inositol auxotroph, ino1, to use exogenous GroPIns as an inositol source, we have isolated mutants (Git−) defective in the uptake and metabolism of GroPIns. One mutant was found to be affected in the gene encoding the transcription factor, SPT7. Mutants of the positive regulatory gene INO2, but not of its partner, INO4, also have the Git− phenotype. Another mutant was complemented by a single open reading frame (ORF) termed GIT1 (glycerophosphoinositol). This ORF consists of 1556 bp predicted to encode a polypeptide of 518 amino acids and 57.3 kD. The predicted Git1p has similarity to a variety of S. cerevisiae transporters, including a phosphate transporter (Pho84p), and both inositol transporters (Itr1p and Itr2p). Furthermore, Git1p contains a sugar transport motif and 12 potential membrane-spanning domains. Transport assays performed on a git1 mutant together with the above evidence indicate that the GIT1 gene encodes a permease involved in the uptake of GroPIns.

scholarly journals Maintenance of Mitochondrial Morphology Is Linked to Maintenance of the Mitochondrial Genome in Saccharomyces cerevisiae

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1147-1156 ◽  
Author(s):  
Theodor Hanekamp ◽  
Mary K Thorsness ◽  
Indrani Rebbapragada ◽  
Elizabeth M Fisher ◽  
Corrine Seebart ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Mitochondrial Dna ◽  
Carbon Sources ◽  
Mitochondrial Morphology ◽  
Yeast Saccharomyces Cerevisiae ◽  
Slow Growth Rate ◽  
Dna Migration ◽  
Mitochondrial Dna Stability ◽  
Extragenic Suppressors

Abstract In the yeast Saccharomyces cerevisiae, certain mutant alleles of YME4, YME6, and MDM10 cause an increased rate of mitochondrial DNA migration to the nucleus, carbon-source-dependent alterations in mitochondrial morphology, and increased rates of mitochondrial DNA loss. While single mutants grow on media requiring mitochondrial respiration, any pairwise combination of these mutations causes a respiratory-deficient phenotype. This double-mutant phenotype allowed cloning of YME6, which is identical to MMM1 and encodes an outer mitochondrial membrane protein essential for maintaining normal mitochondrial morphology. Yeast strains bearing null mutations of MMM1 have altered mitochondrial morphology and a slow growth rate on all carbon sources and quantitatively lack mitochondrial DNA. Extragenic suppressors of MMM1 deletion mutants partially restore mitochondrial morphology to the wild-type state and have a corresponding increase in growth rate and mitochondrial DNA stability. A dominant suppressor also suppresses the phenotypes caused by a point mutation in MMM1, as well as by specific mutations in YME4 and MDM10.

scholarly journals A yeast metabolome-based model for an ecotoxicological approach in the management of lignocellulosic ethanol stillage

2019 ◽  
Vol 6 (1) ◽  
pp. 180718 ◽  
Author(s):  
Luca Roscini ◽  
Lorenzo Favaro ◽  
Laura Corte ◽  
Lorenzo Cagnin ◽  
Claudia Colabella ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Saccharomyces Cerevisiae ◽  
Predictive Model ◽  
Lignocellulosic Ethanol ◽  
Bioethanol Production ◽  
Inhibitor Concentration ◽  
Promising Tool ◽  
Different Types ◽  
The Relationship ◽  
Ecotoxicological Bioassay

Lignocellulosic bioethanol production results in huge amounts of stillage, a potentially polluting by-product. Stillage, rich in heavy metals and, mainly, inhibitors, requires specific toxicity studies to be adequately managed. To this purpose, we applied an FTIR ecotoxicological bioassay to evaluate the toxicity of lignocellulosic stillage. Two weak acids and furans, most frequently found in lignocellulosic stillage, have been tested in different mixtures against three Saccharomyces cerevisiae strains. The metabolomic reaction of the test microbes and the mortality induced at various levels of inhibitor concentration showed that the strains are representative of three different types of response. Furthermore, the relationship between concentrations and FTIR synthetic stress indexes has been studied, with the aim of defining a model able to predict the concentrations of inhibitors in stillage, resulting in an optimized predictive model for all the strains. This approach represents a promising tool to support the ecotoxicological management of lignocellulosic stillage.

scholarly journals Prohibitins Regulate Membrane Protein Degradation by the m-AAA Protease in Mitochondria

1999 ◽  
Vol 19 (5) ◽  
pp. 3435-3442 ◽  
Author(s):  
Gregor Steglich ◽  
Walter Neupert ◽  
Thomas Langer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Membrane Proteins ◽  
Eukaryotic Cells ◽  
Mitochondrial Morphology ◽  
Regulatory Effect ◽  
Inner Membrane ◽  
Functional Activities ◽  
Native Molecular Mass ◽  
Affect Cell Growth

ABSTRACT Prohibitins comprise a protein family in eukaryotic cells with potential roles in senescence and tumor suppression. Phb1p and Phb2p, members of the prohibitin family in Saccharomyces cerevisiae, have been implicated in the regulation of the replicative life span of the cells and in the maintenance of mitochondrial morphology. The functional activities of these proteins, however, have not been elucidated. We demonstrate here that prohibitins regulate the turnover of membrane proteins by the m-AAA protease, a conserved ATP-dependent protease in the inner membrane of mitochondria. The m-AAA protease is composed of the homologous subunits Yta10p (Afg3p) and Yta12p (Rca1p). Deletion ofPHB1 or PHB2 impairs growth of Δyta10 or Δyta12 cells but does not affect cell growth in the presence of the m-AAA protease. A prohibitin complex with a native molecular mass of approximately 2 MDa containing Phb1p and Phb2p forms a supercomplex with them-AAA protease. Proteolysis of nonassembled inner membrane proteins by the m-AAA protease is accelerated in mitochondria lacking Phb1p or Phb2p, indicating a negative regulatory effect of prohibitins on m-AAA protease activity. These results functionally link members of two conserved protein families in eukaryotes to the degradation of membrane proteins in mitochondria.

An investigation into the relationship between anti-Helicobacter pylori and anti-Saccharomyces cerevisiae antibodies in patients with axial spondyloarthritis and Crohn disease

2014 ◽  
Vol 35 (2) ◽  
pp. 359-366 ◽  
Author(s):  
Igor Kunze Rodrigues ◽  
Michele Andrigueti ◽  
Ione Dilma de Oliveira Gil ◽  
Leonardo de Lucca Schiavon ◽  
Kenia Rodrigues de Andrade ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Helicobacter Pylori ◽  
Crohn Disease ◽  
Axial Spondyloarthritis ◽  
The Relationship

The Mitochondrial Nucleoid Protein, Mgm101p, of Saccharomyces cerevisiae Is Involved in the Maintenance of ρ+ and ori/rep-Devoid Petite Genomes but Is Not Required for Hypersuppressive ρ− mtDNA

Genetics ◽  
2002 ◽  
Vol 160 (4) ◽  
pp. 1389-1400
Author(s):  
Xiao Ming Zuo ◽  
G Desmond Clark-Walker ◽  
Xin Jie Chen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Mitochondrial Rna ◽  
Mtdna Copy Number ◽  
Mitochondrial Rna Polymerase ◽  
Mitochondrial Nucleoids ◽  
Mtdna Replication ◽  
Sensitive Allele ◽  
Discriminatory Effect

Abstract The Saccharomyces cerevisiae MGM101 gene encodes a DNA-binding protein targeted to mitochondrial nucleoids. MGM101 is essential for maintenance of a functional ρ+ genome because meiotic segregants, with a disrupted mgm101 allele, cannot undergo more than 10 divisions on glycerol medium. Quantitative analysis of mtDNA copy number in a ρ+ strain carrying a temperature-sensitive allele, mgm101-1, revealed that the amount of mtDNA is halved each cell division upon a shift to the restrictive temperature. These data suggest that mtDNA replication is rapidly blocked in cells lacking MGM101. However, a small proportion of meiotic segregants, disrupted in MGM101, have ρ− genomes that are stably maintained. Interestingly, all surviving ρ− mtDNAs contain an ori/rep sequence. Disruption of MGM101 in hypersuppressive (HS) strains does not have a significant effect on the propagation of HS ρ− mtDNA. However, in petites lacking an ori/rep, disruption of MGM101 leads to either a complete loss or a dramatically decreased stability of mtDNA. This discriminatory effect of MGM101 suggests that replication of ρ+ and ori/rep-devoid ρ− mtDNAs is carried out by the same process. By contrast, the persistence of ori/rep-containing mtDNA in HS petites lacking MGM101 identifies a distinct replication pathway. The alternative mtDNA replication mechanism provided by ori/rep is independent of mitochondrial RNA polymerase encoded by RPO41 as a HS ρ− genome is stably maintained in a mgm101, rpo41 double mutant.

Study on Grain Moisture Detection System Based on the Theory of Dielectric Properties

2013 ◽  
Vol 333-335 ◽  
pp. 1558-1563
Author(s):  
Ling Bin Tan ◽  
Hai Yan Ji
Keyword(s):  
Dielectric Properties ◽  
Detection System ◽  
Capacitive Sensor ◽  
Extreme Environment ◽  
Single Chip ◽  
Display Module ◽  
Temperature Detection ◽  
Grain Moisture ◽  
Moisture Detection ◽  
The Relationship

After studying the relationship between the moisture content of crops and their relative permittivity, the principle of capacitive sensor and the research results of measuring micro-capacitance, this paper summarizes the theoretical basis of dielectric properties of grain, the dielectric properties of wheat, corn and rice, the relationship between the dielectric properties of these crops and their water content. With these theory analyses, the paper gives a full look on the grain moisture detection system by the means of dielectric properties, which consists of six parts, a cylindrical capacitor, signal conditioning circuits, single-chip control module with an A/D converter, temperature detection module, keyboard module and display module. The system is simple, and can adapt to extreme environment and accomplish the rapid detection in manufacturing. A description is given of the principle, its hardware circuit and software programming flow chart.

HeLa cells form focal contacts that are not fibronectin dependent

1984 ◽  
Vol 66 (1) ◽  
pp. 133-145
Author(s):  
J. Morgan ◽  
D. Garrod
Keyword(s):  
Hela Cell ◽  
Hela Cells ◽  
Immunoglobulin G ◽  
Actin Microfilament ◽  
Focal Contacts ◽  
Microfilament Bundles ◽  
High Concentrations ◽  
The Relationship ◽  
Cells Cultured

HeLa cells cultured on glass substrata produce numerous prominent focal contacts, which reside at the termini of actin microfilament bundles. However, very few of the cells stain for fibronectin with specific anti-fibronectin antibody. Moreover, the cells form focal contacts in fibronectin-depleted medium, in the presence of high concentrations of anti-fibronectin immunoglobulin G and in the presence of monensin. No fibronectin synthesis can be detected by [35S]methionine-labelling and immunoprecipitation. The possibility that HeLa cell focal contacts are independent of fibronectin in their formation is discussed in relation to the controversy about the relationship between fibronectin and focal contacts.

Examination of the relationship between parameters to determine electropermeability of Saccharomyces cerevisiae

1999 ◽  
Vol 48 (2) ◽  
pp. 485-488 ◽  
Author(s):  
Masafumi Muraji ◽  
Hiroaki Taniguchi ◽  
Wataru Tatebe ◽  
Hermann Berg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
The Relationship
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