scholarly journals Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Agus Budi Dharmawan ◽  
Shinta Mariana ◽  
Gregor Scholz ◽  
Philipp Hörmann ◽  
Torben Schulze ◽  
...  
Keyword(s):  
Wave Propagation ◽  
Moving Objects ◽  
Light Emitting Diode ◽  
Three Dimensional ◽  
Prorocentrum Minimum ◽  
Distribution Analysis ◽  
Holographic Microscopy ◽  
3D Information ◽  
Point Light

AbstractPerforming long-term cell observations is a non-trivial task for conventional optical microscopy, since it is usually not compatible with environments of an incubator and its temperature and humidity requirements. Lensless holographic microscopy, being entirely based on semiconductor chips without lenses and without any moving parts, has proven to be a very interesting alternative to conventional microscopy. Here, we report on the integration of a computational parfocal feature, which operates based on wave propagation distribution analysis, to perform a fast autofocusing process. This unique non-mechanical focusing approach was implemented to keep the imaged object staying in-focus during continuous long-term and real-time recordings. A light-emitting diode (LED) combined with pinhole setup was used to realize a point light source, leading to a resolution down to 2.76 μm. Our approach delivers not only in-focus sharp images of dynamic cells, but also three-dimensional (3D) information on their (x, y, z)-positions. System reliability tests were conducted inside a sealed incubator to monitor cultures of three different biological living cells (i.e., MIN6, neuroblastoma (SH-SY5Y), and Prorocentrum minimum). Altogether, this autofocusing framework enables new opportunities for highly integrated microscopic imaging and dynamic tracking of moving objects in harsh environments with large sample areas.

scholarly journals 3D monitoring of the surface slippage effect on micro-particle sedimentation by digital holographic microscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Majid Panahi ◽  
Ramin Jamali ◽  
Vahideh Farzam Rad ◽  
Mojtaba Khorasani ◽  
Ahamd Darudi ◽  
...  
Keyword(s):  
Slip Length ◽  
Particle Interaction ◽  
Three Dimensional ◽  
Digital Holographic Microscopy ◽  
Particle Sedimentation ◽  
Micro Particles ◽  
Slippage Effect ◽  
Holographic Microscopy ◽  
3D Information ◽  
Experimental Findings

AbstractIn several phenomena in biology and industry, it is required to understand the comprehensive behavior of sedimenting micro-particles in fluids. Here, we use the numerical refocusing feature of digital holographic microscopy (DHM) to investigate the slippage effect on micro-particle sedimentation near a flat wall. DHM provides quantitative phase contrast and three-dimensional (3D) imaging in arbitrary time scales, which suggests it as an elegant approach to investigate various phenomena, including dynamic behavior of colloids. 3D information is obtained by post-processing of the recorded digital holograms. Through analysis of 3D trajectories and velocities of multiple sedimenting micro-particles, we show that proximity to flat walls of higher slip lengths causes faster sedimentation. The effect depends on the ratio of the particle size to (1) the slip length and (2) its distance to the wall. We corroborate our experimental findings by a theoretical model which considers both the proximity and the particle interaction to a wall of different hydrophobicity in the hydrodynamic forces.

scholarly journals Robust 2D Mapping Integrating with 3D Information for the Autonomous Mobile Robot Under Dynamic Environment

Electronics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1503 ◽  
Author(s):  
Bin Zhang ◽  
Masahide Kaneko ◽  
Hun-ok Lim
Keyword(s):  
Mobile Robot ◽  
Moving Objects ◽  
Three Dimensional ◽  
Dynamic Environment ◽  
Mapping Method ◽  
Research Field ◽  
Working Environment ◽  
3D Mapping ◽  
Occupancy Probability ◽  
3D Information

In order to move around automatically, mobile robots usually need to recognize their working environment first. Simultaneous localization and mapping (SLAM) has become an important research field recently, by which the robot can generate a map while moving around. Both two-dimensional (2D) mapping and three-dimensional (3D) mapping methods have been developed greatly with high accuracy. However, 2D maps cannot reflect the space information of the environment and 3D mapping needs long processing time. Moreover, conventional SLAM methods based on grid maps take a long time to delete the moving objects from the map and are hard to delete the potential moving objects. In this paper, a 2D mapping method integrating with 3D information based on immobile area occupied grid maps is proposed. Objects in 3D space are recognized and their space information (e.g., shapes) and properties (moving objects or potential moving objects like people standing still) are projected to the 2D plane for updating the 2D map. By using the immobile area occupied grid map method, recognized still objects are reflected to the map quickly by updating the immobile area occupancy probability with a high coefficient. Meanwhile, recognized moving objects and potential moving objects are not used for updating the map. The unknown objects are reflected to the 2D map with a lower immobile area occupancy probability so that they can be deleted quickly once they are recognized as moving objects or start to move. The effectiveness of our method is proven by experiments of mapping under dynamic indoor environment using a mobile robot.

3D monitoring of the surface slippage effect on micro-particle sedimentation by digital holographic microscopy

2021 ◽  
Author(s):  
Majid Panahi ◽  
Ramin Jamali ◽  
Vahideh Farzam Rad ◽  
Mojtaba Khorasani ◽  
Ahamd Darudi ◽  
...  
Keyword(s):  
Slip Length ◽  
Particle Interaction ◽  
Three Dimensional ◽  
Digital Holographic Microscopy ◽  
Particle Sedimentation ◽  
Micro Particles ◽  
Slippage Effect ◽  
Holographic Microscopy ◽  
3D Information ◽  
Experimental Findings

Abstract In several phenomena in biology and industry, it is required to understand the comprehensive behavior of sedimenting micro-particles in fluids. Here, we use the numerical refocusing feature of digital holographic microscopy (DHM) to investigate the slippage effect on micro-particle sedimentation near a flat wall. DHM provides quantitative phase contrast and three-dimensional (3D) imaging in arbitrary time scales, which suggests it as an elegant approach to investigate various phenomena, including dynamic behavior of colloids. 3D information is obtained by post-processing of the recorded digital holograms. Through analysis of 3D trajectories and velocities of multiple sedimenting micro-particles, we show that proximity to flat walls of higher slip lengths causes faster sedimentation. The effect depends on the ratio of the particle size to (1) the slip length and (2) its distance to the wall. We corroborate our experimental findings by a theoretical model which considers both the proximity and the particle interaction to a wall of different hydrophobicity in the hydrodynamic forces.

scholarly journals Time-resolved scanning ion conductance microscopy for three-dimensional tracking of nanoscale cell surface dynamics

2021 ◽  
Author(s):  
Samuel M. Leitao ◽  
Barney Drake ◽  
Katarina Pinjusic ◽  
Xavier Pierrat ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Cellular Structures ◽  
Surface Dynamics ◽  
Dynamic Changes ◽  
Ion Conductance ◽  
Time Resolved ◽  
Scanning Ion Conductance Microscopy ◽  
3D Information

Understanding cellular function requires high-resolution information about cellular structures as well as their evolution over time. The major challenge is to obtain three-dimensional (3D) information at nanometer resolution without affecting the viability of the cells and avoiding interference with the process. Here, we develop a scanning ion conductance microscope (SICM) for high-speed and long term imaging that can resolve spatiotemporally diverse processes on the cell membrane. We tracked dynamic changes in live cell morphology with nanometer details and temporal ranges of sub-second to days, imagining diverse processes ranging from endocytosis, micropinocytosis, and mitosis, to bacterial infection and cell differentiation in cancer cells. This technique enables a detailed look at membrane events and may offer insights into cell-cell interactions for infection, immunology, and cancer research.

scholarly journals Digital Documentation of the Saite Tombs in Saqqara

2020 ◽  
Vol 4 (1) ◽  
pp. 16-31
Author(s):  
Matthias Lang Universität Tübingen ◽  
Ramadan Hussein ◽  
Benjamin Glissmann ◽  
Philippe Kluge
Keyword(s):  
Virtual Environments ◽  
Three Dimensional ◽  
3D Models ◽  
Future Generations ◽  
Data Archiving ◽  
Pros And Cons ◽  
Digital Documentation ◽  
3D Information ◽  
Term Data

This paper focuses on the digital documentation techniques employed in the recording of a number of Saite-Persian sarcophagus-tombs in the necropolis of Saqqara (Egypt). In this paper, we discus pros and cons of different three-dimensional technologies for the documentation of a vast site as well as the process of ongoing excavation. We then delve into a discussion of the results and benefits of the employed techniques, particularly understanding the complex spatial relationships of archaeological features both aboveground and underground. Furthermore, we explain how we derive precise scaled and ortho-rectified images of all inscribed walls and objects from the recorded 3D-information in order to produce digital facsimiles. The 3D approach gives us the opportunity to create an exact digital copy of the site, and to record all stages of the excavation. The produced 3D models can be used in various virtual environments in order to give researchers and the general public the possibility to visit and to examine the site from a distance. Also, it is important to note that this paper presents a sustainable long-term data-archiving strategy, since saving the digitally-born data for future generations is an integral part of our Saqqara Saite Tombs Project.

Practical electron tomography

1995 ◽  
Vol 53 ◽  
pp. 742-743
Author(s):  
C.L. Woodcock
Keyword(s):  
Electron Tomography ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
3D Shape ◽  
Computational Resources ◽  
Shape Of Particles

Despite the potential of the technique, electron tomography has yet to be widely used by biologists. This is in part related to the rather daunting list of equipment and expertise that are required. Thanks to continuing advances in theory and instrumentation, tomography is now more feasible for the non-specialist. One barrier that has essentially disappeared is the expense of computational resources. In view of this progress, it is time to give more attention to practical issues that need to be considered when embarking on a tomographic project. The following recommendations and comments are derived from experience gained during two long-term collaborative projects.Tomographic reconstruction results in a three dimensional description of an individual EM specimen, most commonly a section, and is therefore applicable to problems in which ultrastructural details within the thickness of the specimen are obscured in single micrographs. Information that can be recovered using tomography includes the 3D shape of particles, and the arrangement and dispostion of overlapping fibrous and membranous structures.

Custom Presurgical Planning for Midfacial Reconstruction

2020 ◽  
Vol 36 (06) ◽  
pp. 696-702
Author(s):  
Nolan B. Seim ◽  
Enver Ozer ◽  
Sasha Valentin ◽  
Amit Agrawal ◽  
Mead VanPutten ◽  
...  
Keyword(s):  
Multidisciplinary Approach ◽  
Functional Performance ◽  
Three Dimensional ◽  
Free Tissue Transfer ◽  
Long Term Effects ◽  
Presurgical Planning ◽  
Dental Rehabilitation ◽  
Midface Reconstruction ◽  
Reconstructive Algorithm

AbstractResection and reconstruction of midface involve complex ablative and reconstructive tools in head and oncology and maxillofacial prosthodontics. This region is extraordinarily important for long-term aesthetic and functional performance. From a reconstructive standpoint, this region has always been known to present challenges to a reconstructive surgeon due to the complex three-dimensional anatomy, the variable defects created, combination of the medical and dental functionalities, and the distance from reliable donor vessels for free tissue transfer. Another challenge one faces is the unique features of each individual resection defect as well as individual patient factors making each preoperative planning session and reconstruction unique. Understanding the long-term effects on speech, swallowing, and vision, one should routinely utilize a multidisciplinary approach to resection and reconstruction, including head and neck reconstructive surgeons, prosthodontists, speech language pathologists, oculoplastic surgeons, dentists, and/or craniofacial teams as indicated and with each practice pattern. With this in mind, we present our planning and reconstructive algorithm in midface reconstruction, including a dedicated focus on dental rehabilitation via custom presurgical planning.

Analyzing the Impact of X-Ray Tomography on the Reliability of Integrated Circuits

2015 ◽  
Author(s):  
Halit Dogan ◽  
Md Mahbub Alam ◽  
Navid Asadizanjani ◽  
Sina Shahbazmohamadi ◽  
Domenic Forte ◽  
...  
Keyword(s):  
Integrated Circuits ◽  
Integrated Circuit ◽  
3D Imaging ◽  
Three Dimensional ◽  
Serial Sectioning ◽  
Promising Technique ◽  
Flash Memories ◽  
X Ray ◽  
3D Information ◽  
The Impact

Abstract X-ray tomography is a promising technique that can provide micron level, internal structure, and three dimensional (3D) information of an integrated circuit (IC) component without the need for serial sectioning or decapsulation. This is especially useful for counterfeit IC detection as demonstrated by recent work. Although the components remain physically intact during tomography, the effect of radiation on the electrical functionality is not yet fully investigated. In this paper we analyze the impact of X-ray tomography on the reliability of ICs with different fabrication technologies. We perform a 3D imaging using an advanced X-ray machine on Intel flash memories, Macronix flash memories, Xilinx Spartan 3 and Spartan 6 FPGAs. Electrical functionalities are then tested in a systematic procedure after each round of tomography to estimate the impact of X-ray on Flash erase time, read margin, and program operation, and the frequencies of ring oscillators in the FPGAs. A major finding is that erase times for flash memories of older technology are significantly degraded when exposed to tomography, eventually resulting in failure. However, the flash and Xilinx FPGAs of newer technologies seem less sensitive to tomography, as only minor degradations are observed. Further, we did not identify permanent failures for any chips in the time needed to perform tomography for counterfeit detection (approximately 2 hours).

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