scholarly journals Salivary melatonin in oral squamous cell carcinoma patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ivan Salarić ◽  
Ivana Karmelić ◽  
Jasna Lovrić ◽  
Ksenija Baždarić ◽  
Marko Rožman ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Sleep Quality ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Concentration Limit ◽  
Poor Sleep ◽  
Potential Biomarker ◽  
Whole Saliva ◽  
Salivary Melatonin

AbstractMelatonin’s role in circadian rhythm is well documented, as are its’ anti-oxidant, oncostatic and anti-inflammatory properties. Poor sleep quality has been associated as a potential risk factor for several malignancies, including head and neck cancers. The purpose of this study is to determine salivary melatonin (MLT) levels in oral squamous cell carcinoma (OSCC) patients, compare the salivary MLT levels with those in healthy individuals and compare the salivary and serum levels in OSCC patients. Furthermore, the aim is to investigate the potential relationship between sleep quality and salivary MLT levels in OSCC patients. Unstimulated (UWS) and stimulated (SWS) whole saliva was sampled from patients with T1N0M0 and T2N0M0 OSCC (N = 34) and 33 sex and age matched healthy subjects. Serum samples were taken from 11 OSCC patients. Sleep quality was measured using Pittsburgh Sleep Quality Index (PSQI) questionnaire. Melatonin levels in UWS and SWS were significantly higher in the OSCC group. Sleep quality was significantly lower in patients with OSCC (P = 0.0001). ROC analysis was found to be significant (P < 0.001) in evaluating MLT concentration limit in diagnosing OSCC. The expected relationship between sleep quality and salivary MLT levels in OSCC patients was not observed. Our results suggest salivary MLT as a potential biomarker that might facilitate non-invasive detection of early stage OSCC.

Salivary Melatonin in Oral Squamous Cell Carcinoma Patients: A Cross-sectional Study

2020 ◽  
Author(s):  
Ivan Salaric ◽  
Ivana Karmelic ◽  
Jasna Lovric ◽  
Ksenija Bazdaric ◽  
Marko Rozman ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Sleep Quality ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Concentration Limit ◽  
Poor Sleep ◽  
Cross Sectional ◽  
Detailed Medical History ◽  
Salivary Melatonin

Abstract BACKGROUND: Around 55% of oral squamous cell carcinoma (OSCC) patients are diagnosed in the advanced stage. Poor sleep quality has been associated as a potential risk factor for several malignancies, including head and neck cancers. Melatonin’s role in circadian rhythm is well documented, as are its’ anti-oxidant, oncostatic and anti-inflammatory properties. The purpose of this study is to determine salivary melatonin (MLT) levels in OSCC patients, compare the salivary MLT levels with those in healthy individuals and compare the salivary and serum levels in OSCC patients. Furthermore, the aim is to evaluate sleep quality and to investigate the potential relationship between sleep quality and salivary MLT levels in OSCC patients. METHODS: Unstimulated and stimulated saliva were sampled from patients with T1N0M0 and T2N0M0 OSCC (N=34) and 33 sex and age matched healthy subjects. Serum samples were taken from 11 OSCC patients. Detailed medical history was taken before sampling and sleep quality measured using Pittsburgh Sleep Quality Index (PSQI) questionnaire. RESULTS: Melatonin levels in UWS and SWS were significantly higher in the OSCC group. Sleep quality was significantly lower in patients with OSCC (U=249.50; P = 0.0001). ROC analysis was found to be significant (P <0.001) in evaluating the MLT concentration limit in diagnosing OSCC. CONCLUSIONS: The expected relationship between sleep quality and salivary MLT levels in OSCC patients was not observed. Salivary melatonin could present a potential OSCC biomarker. However, larger prospective studies should validate the clinical utility of MLT as an OSCC biomarker.

scholarly journals Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads

2015 ◽  
Vol 35 (3) ◽  
Author(s):  
Wei-Peng Jiang ◽  
Zhen Wang ◽  
Li-Xin Xu ◽  
Xin Peng ◽  
Feng Chen
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cation Exchange ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Magnetic Beads ◽  
Diagnostic Model ◽  
Finger Print ◽  
Whole Saliva ◽  
Weak Cation Exchange

Whole saliva (WS) was used for diagnosis of oral squamous cell carcinoma (OSCC); two polypeptides may be used for OSCC diagnosis.

Animal model and bioinformatics analyses suggest the TIMP1/MMP9 axis as a potential biomarker in oral squamous cell carcinoma

2020 ◽  
Vol 59 (11) ◽  
pp. 1302-1316
Author(s):  
Guoqiang Xu ◽  
Jianing Wei ◽  
Bing Huangfu ◽  
Jiping Gao ◽  
Xiaotang Wang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Animal Model ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Bioinformatics Analyses ◽  
Potential Biomarker

Role of pretreatment neutrophil-to-lymphocyte ratio as an independent prognostic factor in oral squamous cell carcinoma patients: A prospective study in a tertiary care center

2018 ◽  
Vol 3 (2) ◽  
pp. 37
Author(s):  
Shashwat Tiwari ◽  
Shivani Nautiyal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Prognostic Index ◽  
Prognostic Scores ◽  
Lymphocyte Ratio ◽  
Potential Biomarker ◽  
The Absolute

<p><strong>Background:</strong> More recently, established systemic inflammation-based prognostic scores have been explored extensively, such as neutrophil-lymphocyte ratio (NLR) and serum C-reactive protein. We postulated that NLR might be a readily available and inexpensive objective prognostic index that could be used in daily oncologic clinical practice and could help to stratify patients in clinical trials.<br /><strong>Materials and Methods:</strong> In total, there were 150 patients with oral squamous cell carcinoma (OSCC) treated at GSVM Medical College, Kanpur, between October 2012 and January 2015 whose clinical information and laboratory parameters were obtained. The NLR was determined by dividing the absolute neutrophil count by the absolute lymphocyte count, and the NLR data were then dichotomized and divided into two groups as NLR low and high. <strong>Conclusion:</strong> Our findings reported herein demonstrated that pretreatment NLR is a potential biomarker for predicting the overall survival in OSCC patients. Combined with other markers, NLR may be used in decision-making and the selection of treatment modality in patients with oral SCC.</p>

scholarly journals Overexpression of Stathmin in Oral Squamous Cell Carcinoma, its Association with Histomorphological Features and Pathological Staging

2021 ◽  
Author(s):  
Nabanita Barma ◽  
Gopinath Barui ◽  
Anadi Roy Chowdhury
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Clinicopathological Parameters ◽  
Pathological Staging ◽  
Potential Biomarker ◽  
Tobacco Chewing

Introduction: Stathmin, one of the most important intracellular phosphoprotein that plays the vital role in the regulation of cell cycle and cell proliferation. It acts by disassembly of Microtubules (MT) that helps in the formation of mitotic spindle. It is overexpressed in various human cancers. Aim: To investigate the expression status of stathmin and its relation with clinicopathologic significance in Oral Squamous Cell Carcinoma (OSCC) and its association with age, sex, tobacco chewing, histomorphological features and pathological staging. Materials and Methods: A descriptive and observational study in cross-sectional design was conducted in a tertiary care centre of Kolkata, West Bengal, India, from January 2020 to March 2020 in the Department of Pathology of RG Kar Medical College and Hospital in collaboration with Department of Oto-Rhino-Laryngology of the same institute. Stathmin expression was assessed by immunohistochemistry (IHC) in 28 OSCC cases. The association between stathmin expression and clinicopathological parameters like age, sex, tobacco chewing, tumour site, histomorphological type, Tumor Nodes Metastases (TNM) stage and Worst Pattern Of Invasion (WPOI) were evaluated on the basis of Chi-square test and Fisher’s-exact test (software Statistical Package for the Social Sciences (SPSS) V 25.0). Results: Stathmin was overexpressed in 18 cases out of 28 cases of OSCC. In this study a positive association was observed between stathmin expression and age group, history of tobacco chewing, advanced T stage, advanced pTNM staging and WPOI of OSCC. Conclusion: The study suggests that overexpression of stathmin could contribute to cancer progression. There is a higher likelihood that stathmin may be used as a potential biomarker as well as therapeutic target for OSCC.

Assessment of MicroRNA-15a and MicroRNA-16-1 Salivary Level in Oral Squamous Cell Carcinoma Patients

MicroRNA ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Soheila Manifar ◽  
Maryam Koopaie ◽  
Shahab Shokouhi Lahiji
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Micro Rna ◽  
Control Group ◽  
Healthy Individuals ◽  
Oral Cancers ◽  
Whole Saliva

Background: Squamous Cell Carcinoma (SCC) includes more than 90% of malignancies of the oral cavity. Early diagnosis could effectively improve patients' quality of life and treatment outcomes of oral cancers. MicroRNAs as non-encoding genes have great potential to initiate or suppress cancer progression. Recent studies have shown that disruption of micro-RNA regulation is a common occurrence in cancers. Objective: This study set out to evaluate the expression of microRNA-15a (miR-15a) and microRNA-16-1 (miR-16-1) in the saliva of Oral Squamous Cell Carcinoma (OSCC) patients in comparison with a healthy control group. Methods: This case-control study was performed on fifteen patients with OSCC and fifteen healthy volunteers as the control group. A 5 ml of non-stimulating whole saliva was collected by spitting method from patients and controls and stored at -70oC. The expression of miR-15a and miR-16-1 was investigated using quantitative Reverse-Transcription Polymerase Chain Reaction (RT-qPCR). Results: MiR-15a and miR-16-1 were downregulated in OSCC patients compared with the control group (p<0.001). The sensitivity of miR-15a and miR-16-1 in differentiating OSCC patients from healthy individuals was 93.3% and 86.67%, respectively, and their specificity was 86.67% and 92.33%, respectively. The diagnostic accuracy of miR-15a was 90%, and miR-16-1 was 93.3%. Conclusion: The present study showed a decrease in the relative expression of miR-15a and miR-16-1 in OSCC patients compared with healthy individuals. It is probable to introduce salivary values of miR-15a and miR-16-1 as a non-invasive tool for early detection of OSCC. Decreased expression of miR-15a and miR-16-1 in OSCC indicates the possible effective role of these genes in OSCC etiopathogenesis.

Cripto 1, a potential biomarker for oral squamous cell carcinoma

Oral Oncology ◽  
2019 ◽  
Vol 90 ◽  
pp. 153-154 ◽  
Author(s):  
Anu Jain ◽  
Sumanth Mallupattu ◽  
Reetu Thakur ◽  
Sushmita Ghoshal ◽  
Arnab Pal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Potential Biomarker

scholarly journals HISTOPATHOLOGICAL GRADING OF ORAL SQUAMOUS CELL CARCINOMA BY MICRONUCLEI ASSAY, A POTENTIAL BIOMARKER IN ORAL EXFOLIATED CELLS

2021 ◽  
pp. 95-99
Author(s):  
Noratan Jatiya ◽  
Vijay Rajak ◽  
Savita Soni
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tobacco Consumption ◽  
Control Group ◽  
Exfoliated Cells ◽  
Potential Biomarker ◽  
The Difference ◽  
Histopathological Grading

Background: Micronucleus is a microscopically visible round or oval cytoplasmic chromatin mass in the extra nuclear vicinity, originated from aberrant mitosis, which consists of eccentric chromosomes that have failed to reach spindle poles during mitosis and are used as biomarkers for assessment of DNA damage. Micronuclei in exfoliated oral epithelial cells have been shown in some studies to correlate with severity of this genotoxic damage. This severity can be measured in terms of grading of the lesions. Aim: To correlate frequency of micronuclei (MN) in oral exfoliated cells in clinically diagnosed cases of oral squamous cell carcinoma (OSCC) followed by a histopathological grading. Materials and Methods : The study subjects consisted of clinically diagnosed cases of OSCC. Healthy subjects without any tobacco consumption habits formed the control group.The cytosmears from both groups were stained with rapid Papanicolaou stain. MN were identified according to the criteria given by Tolbert et al. Results: The frequency of MN was three to four times higher in patients with OSCC as compared to patients in the control group and the difference was found to be highly significant. In 75% cases, the cytological grade as determined by the frequency of micronuclei correlated with the histopathological grade and this observation was statistically significant. Conclusions : The MN is potentially an excellent candidate to serve as such a biomarker for prediction of the grade of OSCC.

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