scholarly journals Wuchereria bancrofti filaria activates human dendritic cells and polarizes T helper 1 and regulatory T cells via toll-like receptor 4

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Suprabhat Mukherjee ◽  
Anupama Karnam ◽  
Mrinmoy Das ◽  
Santi P. Sinha Babu ◽  
Jagadeesh Bayry
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Regulatory T Cells ◽  
Wuchereria Bancrofti ◽  
Toll Like Receptor ◽  
T Helper ◽  
T Helper 1 ◽  
Toll Like Receptor 4 ◽  
Human Dendritic Cells

Immunopotent Polysaccharides of Different Sources Selectively Activate Human Dendritic Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3873-3873
Author(s):  
Godfrey ChiFung Chan ◽  
W.K. Chan ◽  
H.K. Law ◽  
Z.B. Lin ◽  
Y.L. Lau
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
T Helper ◽  
T Helper 1 ◽  
Xtt Assay ◽  
Peripheral Blood Mononuclear ◽  
Human Dendritic Cells

Abstract Background: Purified polysaccharides extracted from plants and fungi have been shown to induce immune responses in-vivo and vitro over the past decade. Currently, most of these polysaccharides are found to be glucan but with different branch structure and sizes. Their relative potency and effect on human immune cells remains unknown. This study aims to compare their relative effect on human dendritic cell, the most potent antigen presenting cell. Materials & Methods: We selected 2 prototypes of purified polysaccharides extracted from: 1) Ganoderma lucidum (GL, Lingzhi, Reishi) mycelium, a widely used herb with long and branching β (1® 3), (1® 6) glucan structure (provided by Prof. Lin ZB, Beijing) and 2) Barley with shorter and different branching β (1® 3), (1® 4) structure (provided by Prof. Cheung VNK, NY). Their characteristics and chemical properties had been reported previously. Human peripheral blood mononuclear cells (PBMCs) proliferation was studied by XTT assay. Human dendritic cells (DCs) were derived from monocytes and maturation of DCs were determined by: a) immunophenotypic shift using flow cytometer; 2) dextran endocytosis assay and 3) mixed lymphocytes reaction. Cytokine secretions were determined by ELISA test. Comparisons between means were by nonparametric Student’s t test (2-tailed). Results: We found that purified polysaccharides from GL but not barley could induce PBMCs proliferation and maturation of DCs. GL polysaccharides could enhance phenotypic and functional maturation of DCs with significant IL-12 and IL-10 production. DCs were relatively inert to Barley glucans stimulation. However, both polysaccharides did not polarize T cells into the direction of T helper 1, T helper 2 or regulatory T cells. Conclusions: Our study shown that purified polysaccharides extracted from plants and fungi have different effect on human DCs and their potency and effects are probably affected by their respective sources and structures.

scholarly journals Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization

2005 ◽  
Vol 102 (8) ◽  
pp. 2880-2885 ◽  
Author(s):  
M. Mohamadzadeh ◽  
S. Olson ◽  
W. V. Kalina ◽  
G. Ruthel ◽  
G. L. Demmin ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
T Helper ◽  
T Helper 1 ◽  
Human Dendritic Cells

scholarly journals CD4+CD25+Foxp3+ regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway

2020 ◽  
Vol 48 (12) ◽  
pp. 030006052098094
Author(s):  
Shuang Qin ◽  
Li Li ◽  
Jia Liu ◽  
Jinrui Zhang ◽  
Qing Xiao ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Inflammatory Response ◽  
Mouse Model ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Unexplained Recurrent Spontaneous Abortion ◽  
Tlr4 Antagonist

Objective The present study aimed to evaluate the effects of cluster of differentiation (CD)4+CD25+ forkhead box p3 (Foxp3)+ regulatory T cells (Tregs) on unexplained recurrent spontaneous abortion (URSA) and the associated mechanisms. Methods The proportion of CD4+CD25+Foxp3+ Tregs and inflammatory cytokine concentrations in the peripheral blood of women with URSA were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. CBA/JxDBA/2J mating was used to establish an abortion-prone mouse model and the model mice were treated with the Toll-like receptor 4 (TLR4) antagonist E5564 and the TLR4 agonist lipopolysaccharide. Results The proportion of CD4+CD25+Foxp3+ Tregs was decreased and the inflammatory response was increased in women with URSA. In the abortion-prone mouse model, E5564 significantly increased the proportion of CD4+CD25+Foxp3+ Tregs, decreased the inflammatory response, and increased Foxp3 mRNA and protein expression. Lipopolysaccharide had adverse effects on the abortion-prone model. Conclusions These data suggest that CD4+CD25+Foxp3+ Tregs regulate immune homeostasis in URSA via the TLR4/nuclear factor-κB pathway, and that the TLR4 antagonist E5564 may be a novel and potential drug for treating URSA.

scholarly journals Human Dendritic Cells Produce TGF-β1 under the Influence of Lung Carcinoma Cells and Prime the Differentiation of CD4+CD25+Foxp3+Regulatory T Cells

2009 ◽  
Vol 182 (5) ◽  
pp. 2795-2807 ◽  
Author(s):  
Ingrid E. Dumitriu ◽  
Donald R. Dunbar ◽  
Sarah E. Howie ◽  
Tariq Sethi ◽  
Christopher D. Gregory
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Regulatory T Cells ◽  
Lung Carcinoma ◽  
Carcinoma Cells ◽  
Lung Carcinoma Cells ◽  
Human Dendritic Cells ◽  
Tgf Β1

scholarly journals CD86 and IL-12p70 Are Key Players for T Helper 1 Polarization and Natural Killer Cell Activation by Toll-Like Receptor-Induced Dendritic Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e44266 ◽  
Author(s):  
Felix S. Lichtenegger ◽  
Katharina Mueller ◽  
Bettina Otte ◽  
Barbara Beck ◽  
Wolfgang Hiddemann ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Natural Killer Cell ◽  
Natural Killer ◽  
Cell Activation ◽  
Killer Cell ◽  
Toll Like Receptor ◽  
T Helper ◽  
T Helper 1 ◽  
Natural Killer Cell Activation ◽  
Key Players

scholarly journals Retinoic acid primes human dendritic cells to induce gut-homing, IL-10-producing regulatory T cells

2014 ◽  
Vol 8 (2) ◽  
pp. 265-278 ◽  
Author(s):  
G Bakdash ◽  
L TC Vogelpoel ◽  
T MM van Capel ◽  
M L Kapsenberg ◽  
E C de Jong
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Retinoic Acid ◽  
Regulatory T Cells ◽  
Human Dendritic Cells

Curcumin converts Foxp3+ regulatory T cells to T helper 1 cells in patients with lung cancer

2017 ◽  
Vol 119 (2) ◽  
pp. 1420-1428 ◽  
Author(s):  
Jian Y. Zou ◽  
Chun H. Su ◽  
Hong H. Luo ◽  
Yi Y. Lei ◽  
Bo Zeng ◽  
...  
Keyword(s):  
Lung Cancer ◽  
T Cells ◽  
Regulatory T Cells ◽  
T Helper ◽  
T Helper 1 ◽  
T Helper 1 Cells

scholarly journals Antagonistic nature of T helper 1/2 developmental programs in opposing peripheral induction of Foxp3+ regulatory T cells

2007 ◽  
Vol 104 (46) ◽  
pp. 18169-18174 ◽  
Author(s):  
J. Wei ◽  
O. Duramad ◽  
O. A. Perng ◽  
S. L. Reiner ◽  
Y.-J. Liu ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
T Helper ◽  
T Helper 1 ◽  
Developmental Programs

scholarly journals Inhibition of NF-κB and Oxidative Pathways in Human Dendritic Cells by Antioxidative Vitamins Generates Regulatory T Cells

2005 ◽  
Vol 174 (12) ◽  
pp. 7633-7644 ◽  
Author(s):  
Peng H. Tan ◽  
Pervinder Sagoo ◽  
Cliburn Chan ◽  
John B. Yates ◽  
Jamie Campbell ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Regulatory T Cells ◽  
Antioxidative Vitamins ◽  
Human Dendritic Cells
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