scholarly journals A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (Glycine max)

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Arun Kumaran Anguraj Vadivel ◽  
Tim McDowell ◽  
Justin B. Renaud ◽  
Sangeeta Dhaubhadel
Keyword(s):  
Transcription Factor ◽  
Hairy Roots ◽  
Defense Mechanisms ◽  
Biosynthetic Pathway ◽  
Bzip Transcription Factor ◽  
Myb Transcription Factor ◽  
In Planta ◽  
Rnai Silencing ◽  
Protein Protein Interaction ◽  
Soybean Roots

AbstractGmMYB176 is an R1 MYB transcription factor that regulates multiple genes in the isoflavonoid biosynthetic pathway, thereby affecting their levels in soybean roots. While GmMYB176 is important for isoflavonoid synthesis, it is not sufficient for the function and requires additional cofactor(s). The aim of this study was to identify the GmMYB176 interactome for the regulation of isoflavonoid biosynthesis in soybean. Here, we demonstrate that a bZIP transcription factor GmbZIP5 co-immunoprecipitates with GmMYB176 and shows protein–protein interaction in planta. RNAi silencing of GmbZIP5 reduced the isoflavonoid level in soybean hairy roots. Furthermore, co-overexpression of GmMYB176 and GmbZIP5 enhanced the level of multiple isoflavonoid phytoallexins including glyceollin, isowighteone and a unique O-methylhydroxy isoflavone in soybean hairy roots. These findings could be utilized to develop biotechnological strategies to manipulate the metabolite levels either to enhance plant defense mechanisms or for human health benefits in soybean or other economically important crops.

scholarly journals An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae

2010 ◽  
Vol 10 (1) ◽  
pp. 50 ◽  
Author(s):  
Kui Lin-Wang ◽  
Karen Bolitho ◽  
Karryn Grafton ◽  
Anne Kortstee ◽  
Sakuntala Karunairetnam ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Biosynthetic Pathway ◽  
Myb Transcription Factor ◽  
Anthocyanin Biosynthetic Pathway ◽  
R2r3 Myb

scholarly journals A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e70778 ◽  
Author(s):  
Wenjun Huang ◽  
Wei Sun ◽  
Haiyan Lv ◽  
Ming Luo ◽  
Shaohua Zeng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Biosynthetic Pathway ◽  
Myb Transcription Factor ◽  
Flavonoid Biosynthetic Pathway ◽  
Epimedium Sagittatum ◽  
R2r3 Myb

scholarly journals Genome-Wide Identification and Characterization of Wheat 14-3-3 Genes Unravels the Role of TaGRF6-A in Salt Stress Tolerance by Binding MYB Transcription Factor

2021 ◽  
Vol 22 (4) ◽  
pp. 1904
Author(s):  
Wenna Shao ◽  
Wang Chen ◽  
Xiaoguo Zhu ◽  
Xiaoyi Zhou ◽  
Yingying Jin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Stress Tolerance ◽  
Interaction Analysis ◽  
Family Members ◽  
Myb Transcription Factor ◽  
Alkaline Stress ◽  
Protein Protein Interaction ◽  
Phylogenetic Comparison ◽  
Fundamental Information

14-3-3 proteins are a large multigenic family of general regulatory factors (GRF) ubiquitously found in eukaryotes and play vital roles in the regulation of plant growth, development, and response to stress stimuli. However, so far, no comprehensive investigation has been performed in the hexaploid wheat. In the present study, A total of 17 potential 14-3-3 gene family members were identified from the Chinese Spring whole-genome sequencing database. The phylogenetic comparison with six 14-3-3 families revealed that the majority of wheat 14-3-3 genes might have evolved as an independent branch and grouped into ε and non-ε group using the phylogenetic comparison. Analysis of gene structure and motif indicated that 14-3-3 protein family members have relatively conserved exon/intron arrangement and motif composition. Physical mapping showed that wheat 14-3-3 genes are mainly distributed on chromosomes 2, 3, 4, and 7. Moreover, most 14-3-3 members in wheat exhibited significantly down-regulated expression in response to alkaline stress. VIGS assay and protein-protein interaction analysis further confirmed that TaGRF6-A positively regulated slat stress tolerance by interacting with a MYB transcription factor, TaMYB64. Taken together, our findings provide fundamental information on the involvement of the wheat 14-3-3 family in salt stress and further investigating their molecular mechanism.

scholarly journals Redox Regulation of an AP-1-Like Transcription Factor, YapA, in the Fungal Symbiont Epichloë festucae

2013 ◽  
Vol 12 (10) ◽  
pp. 1335-1348 ◽  
Author(s):  
Gemma M. Cartwright ◽  
Barry Scott
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Redox Regulation ◽  
Bzip Transcription Factor ◽  
Wild Type ◽  
In Planta ◽  
Content Type ◽  
Epichloë Festucae ◽  
Whole Plant ◽  
Cellular Phenotypes

ABSTRACTOne of the central regulators of oxidative stress inSaccharomyces cerevisiaeis Yap1, a bZIP transcription factor of the AP-1 family. In unstressed cells, Yap1 is reduced and cytoplasmic, but in response to oxidative stress, it becomes oxidized and accumulates in the nucleus. To date, there have been no reports on the role of AP-1-like transcription factors in symbiotic fungi. An ortholog of Yap1, named YapA, was identified in the genome of the grass symbiontEpichloë festucaeand shown to complement anS. cerevisiaeΔyap1mutant. Hyphae of theE. festucaeΔyapAstrain were sensitive to menadione and diamide but resistant to H2O2, KO2, andtert-butyl hydroperoxide (t-BOOH). In contrast, conidia of the ΔyapAstrain were very sensitive to H2O2and failed to germinate. Using a PcatA-eGFPdegron-tagged reporter, YapA was shown to be required for expression of a spore-specific catalase gene,catA. Although YapA-EGFP localized to the nucleus in response to host reactive oxygen species during seedling infection, there was no difference in whole-plant and cellular phenotypes of plants infected with the ΔyapAstrain compared to the wild-type strain. Homologs of theS. cerevisiaeandSchizosaccharomyces pomberedox-sensing proteins (Gpx3 and Tpx1, respectively) did not act as redox sensors for YapA inE. festucae. In response to oxidative stress, YapA-EGFP localized to the nuclei ofE. festucaeΔgpxC, ΔtpxA, and ΔgpxCΔtpxAcells to the same degree as that in wild-type cells. These results show thatE. festucaehas a robust system for countering oxidative stress in culture andin plantabut that Gpx3- or Tpx1-like thiol peroxidases are dispensable for activation of YapA.

scholarly journals Functional Role of Aspergillus carbonarius AcOTAbZIP Gene, a bZIP Transcription Factor within the OTA Gene Cluster

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 111
Author(s):  
Donato Gerin ◽  
Federica Garrapa ◽  
Ana-Rosa Ballester ◽  
Luis González-Candelas ◽  
Rita Milvia De Miccolis Angelini ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Cluster ◽  
Functional Role ◽  
Biosynthetic Pathway ◽  
Fungal Species ◽  
Bzip Transcription Factor ◽  
Binding Motif ◽  
Aspergillus Carbonarius ◽  
Transcription Factor Binding Motif ◽  
Grape Berries

Aspergillus carbonarius is the principal fungal species responsible for ochratoxin A (OTA) contamination of grapes and derived products in the main viticultural regions worldwide. In recent years, co-expressed genes representing a putative-OTA gene cluster were identified, and the deletion of a few of them allowed the partial elucidation of the biosynthetic pathway in the fungus. In the putative OTA-gene cluster is additionally present a bZIP transcription factor (AcOTAbZIP), and with this work, A. carbonarius ΔAcOTAbZIP strains were generated to study its functional role. According to phylogenetic analysis, the gene is conserved in the OTA-producing fungi. A Saccharomyces cerevisiae transcription factor binding motif (TFBM) homolog, associated with bZIP transcription factors was present in the A. carbonarius OTA-gene cluster no-coding regions. AcOTAbZIP deletion results in the loss of OTA and the intermediates OTB and OTβ. Additionally, in ΔAcOTAbZIP strains, a down-regulation of AcOTApks, AcOTAnrps, AcOTAp450, and AcOTAhal genes was observed compared to wild type (WT). These results provide evidence of the direct involvement of the AcOTAbZIP gene in the OTA biosynthetic pathway by regulating the involved genes. The loss of OTA biosynthesis ability does not affect fungal development as demonstrated by the comparison of ΔAcOTAbZIP strains and WT strains in terms of vegetative growth and asexual sporulation on three different media. Finally, no statistically significant differences in virulence were observed among ΔAcOTAbZIP strains and WT strains on artificially inoculated grape berries, demonstrating that OTA is not required by A. carbonarius for the pathogenicity process.

scholarly journals The MYB transcription factor Emission of Methyl Anthranilate 1 stimulates emission of methyl anthranilate from Medicago truncatula hairy roots

2019 ◽  
Vol 99 (4) ◽  
pp. 637-654
Author(s):  
Jacob Pollier ◽  
Nathan De Geyter ◽  
Tessa Moses ◽  
Benoît Boachon ◽  
José M. Franco‐Zorrilla ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Medicago Truncatula ◽  
Hairy Roots ◽  
Myb Transcription Factor ◽  
Methyl Anthranilate
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